Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing

Abstract

The dynamic nature of micellar nanostructures is employed to form a self-assembled Forster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.

Interface Engineering of Bi‐Fluorescence Molecules for High‐Performance Data Encryption and Ultralow UV‐Light Detection

“…Therefore, OA is one of the excellent and efficient candidates for regulating excited-state luminescence. [41][42][43][44] Here, we successfully regulated the luminescence of a series of difluoroboron β-diketonate (DFBK) chromophores that were emitted from different electronically excited states at the interface of oleic acid molecules. Selective excitation of OA in the OA-DFBK composites led to blue luminescence, which could be attributed to the locally excited state emission of the DFBK (Figure 1a).…”

“…Therefore, OA is one of the excellent and efficient candidates for regulating excited‐state luminescence. [ 41–44 ]…”

Recent Development in Plasmonic Nanobiosensors for Viral DNA/RNA Biomarkers

“…This system can significantly reduce the background signal and improve the detection sensitivity to as low as 24.57 nmol/L. Vafaei et al developed a micellar nanostructure-based DNA sensing system composed of lipid and two nucleic acid probes with a donor and acceptor [ 49 ]. Before the target DNA-binding reaction, two complimentary DNA strands with Cy3 and Cy5 were encapsulated into each two micelle structures.…”

DNA‐Mediated Membrane Fusion and Its Biological Applications: Sensing, Reaction Control and Drug Delivery

“…[79] Recently, Mendes et al reported a signal amplification strategy for FRET sensing of DNA via DNA-mediated micelles fusion (Figure 5d). [80] In their approach, the lipid-DNA conjugates modified with FRET pairs are incorporated into micellar scaffolds, and recognition of the target complementary DNA brings the two micelles into close contact, thereby facilitating micelle content mixing and resulting in FRET. They obtained ~20-fold enhancement in the sensitivity compared to the conventional single donor-acceptor design.…”