Formulation of alginate beads to encapsulated kaffir lime callus

Prabowo, Beni Hendro; Damayanti, Frisca; Fajarina, Sudewi; Indrianto, Ari; Sasongko, Aries Bagus; Tunjung, Woro Anindito Sri

doi:10.1063/5.0016416

Cited by 1 publication

(5 citation statements)

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“…Murashige and Skoog (MS) media with two ppm of 2,4-Dichlorophenoxy acetic acid sodium salt monohydrate (Sigma) was used as the induction and subculture medium. This method was based on our previous study (Prabowo et al, 2020).…”

Section: Callus Inductionmentioning

confidence: 99%

“…The 21 days of G1 callus was cultured inside the bottle contain MS media with two ppm of 2,4-D then preserved at 4°C for 21 days. This method according to our previous study (Prabowo et al, 2020). After the preservation period, callus is being recultured for 14 days at room temperature.…”

Section: Callus Preservation In 4°c Without Alginate Encapsulationmentioning

confidence: 99%

“…Then the solution was sterilized by using an autoclave. This method according to our previous study (Prabowo et al, 2020). CaCl2.2H2O (Merck) solution 0.2M was used as an alginate hardener solution.…”

Section: Preparation Of Callus Encapsulationmentioning

confidence: 99%

“…To prepare 0.2M (w/v) CaCl2.2H2O (MW:147.02), 2.9404g of CaCl2.2H2O dissolved in 100mL aquadest and sterilized by autoclave. This method according to our previous study (Prabowo et al, 2020).…”

Section: Preparation Of Callus Encapsulationmentioning

confidence: 99%

“…The capsule was preserved inside the petri dish and maintained in 4°C (refrigerator) for 21 days. This method according to our previous study (Prabowo et al, 2020). After preservation, callus is recultured in a new medium for 14 days and kept at room temperature.…”

Section: Callus Preservation In 4°c With Alginate Encapsulationmentioning

confidence: 99%

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Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation

Tunjung

Fajarina²,

Prabowo³

et al. 2021

Indonesian J Pharm

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Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus.

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Section: Callus Inductionmentioning

confidence: 99%

Section: Callus Preservation In 4°c Without Alginate Encapsulationmentioning

confidence: 99%

Section: Preparation Of Callus Encapsulationmentioning

confidence: 99%

Section: Preparation Of Callus Encapsulationmentioning

confidence: 99%

Section: Callus Preservation In 4°c With Alginate Encapsulationmentioning

confidence: 99%

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