Formulation and characterization of silk sericin–PVA scaffold crosslinked with genipin

Aramwit, Pornanong; Siritientong, Tippawan; Kanokpanont, Sorada; Srichana, Teerapol

doi:10.1016/j.ijbiomac.2010.08.015

Cited by 136 publications

(79 citation statements)

References 47 publications

(54 reference statements)

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“…In all cases, protein release from scaffolds occurred simultaneously with mass loss and all of the scaffolds released sericin in a time-dependent manner. The release of PVA also occurred simultaneously with sericin as shown in previous study (12). The gamma-irradiated scaffold released the highest amount of sericin, which was even higher than that released from the untreated sample.…”

Section: Discussionsupporting

confidence: 81%

“…Protein-based materials such as silk protein, fibroin (fibrous protein), and sericin (degumming protein) have generated much interest in the biomedical and biotechnological fields due to their unique properties (5)(6)(7)(8). Many researchers have successfully formed fibroin scaffolds for vascular tissue, connective tissue, and bone regeneration (9-11) whereas sericin scaffolds have been applied in skin substitution (12). Sericin is considered to be a waste material in textile manufacturing.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds

2011

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Abstract. Protein-based biomaterials respond differently to sterilization methods. Since protein is a complex structure, heat, or irradiation may result in the loss of its physical or biological properties. Recent investigations have shown that sericin, a degumming silk protein, can be successfully formed into a 3-D scaffolds after mixing with other polymers which can be applied in skin tissue engineering. The objective of this study was to investigate the effectiveness of ethanol, ethylene oxide (EtO) and gamma irradiation on the sterilization of sericin scaffolds. The influence of these sterilization methods on the physical properties such as pore size, scaffold dimensions, swelling and mechanical properties, as well as the amount of sericin released from sericin/polyvinyl alcohol/glycerin scaffolds, were also investigated. Ethanol treatment was ineffective for sericin scaffold sterilization whereas gamma irradiation was the most effective technique for scaffold sterilization. Moreover, ethanol also caused significant changes in pore size resulting from shrinkage of the scaffold. Gamma-irradiated samples exhibited the highest swelling property, but they also lost the greatest amount of weight after immersion for 24 h compared with scaffolds obtained from other sterilization methods. The results of the maximum stress test and Young's modulus showed that gamma-irradiated and ethanol-treated scaffolds are more flexible than the EtO-treated and untreated scaffolds. The amount of sericin released, which was related to its collagen promoting effect, was highest from the gamma-irradiated scaffold. The results of this study indicate that gamma irradiation should have the greatest potential for sterilizing sericin scaffolds for skin tissue engineering.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds

2011

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“…Many formulations of porous scaffolds fabricated from either natural or synthetic materials have been developed for accelerated wound healing [Agrawal and Ray, 2001;Babaeijandaghi et al, 2010;Steinstraesser et al, 2010]. Our previous study reported that sericin blended with PVA formed a three-dimensional scaffold with a fragile structure [Aramwit et al, 2010b]. Glycerin (as a plasticizer) and genipin (as a crosslinking agent) were added to make a strong and stable matrix.…”

Section: Discussionmentioning

confidence: 99%

“…Glycerin (as a plasticizer) and genipin (as a crosslinking agent) were added to make a strong and stable matrix. The chemical and physical properties of sericin/ PVA scaffolds with or without glycerin and genipin at various concentrations were fully characterized [Aramwit et al, 2010b]. In this study, the physical properties and biological potential for wound healing of the genipin-crosslinked sericin/PVA scaffolds were of interest.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, scaffolds produced from combinations of natural and synthetic materials have received increasing attention [Mandal et al, 2011;Kundu et al, 2012]. We previously combined sericin, poly(vinyl alcohol) (PVA), glycerin (as a plasticizer) and genipin (as a crosslinking agent) to fabricate scaffolds with good physical properties [Aramwit et al, 2010b;Siritientong et al, 2011]. Based on the results of this previous study, the strong and stable genipin-crosslinked scaffolds appeared to mechanically support the wound and control the release of sericin to accelerate wound healing.…”

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confidence: 99%

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Accelerated Healing of Full-Thickness Wounds by Genipin-Crosslinked Silk Sericin/PVA Scaffolds

Aramwit

Siritienthong

Srichana

et al. 2013

Cells Tissues Organs

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Silk sericin has recently been studied for its advantageous biological properties, including its ability to promote wound healing. This study developed a delivery system to accelerate the healing of full-thickness wounds. Three-dimensional scaffolds were fabricated from poly(vinyl alcohol) (PVA), glycerin (as a plasticizer) and genipin (as a crosslinking agent), with or without sericin. The physical and biological properties of the genipin-crosslinked sericin/PVA scaffolds were investigated and compared with those of scaffolds without sericin. The genipin-crosslinked sericin/PVA scaffolds exhibited a higher compressive modulus and greater swelling in water than the scaffolds without sericin. Sericin also exhibited controlled release from the scaffolds. The genipin-crosslinked sericin/PVA scaffolds promoted the attachment and proliferation of L929 mouse fibroblasts. After application to full-thickness rat wounds, the wounds treated with genipin-crosslinked sericin/PVA scaffolds showed a significantly greater reduction in wound size, collagen formation and epithelialization compared with the control scaffolds without sericin but lower numbers of macrophages and multinucleated giant cells. These results indicate that the delivery of sericin from the novel genipin-crosslinked scaffolds efficiently healed the wound. Therefore, these genipin-crosslinked sericin/PVA scaffolds represent a promising candidate for the accelerated healing of full-thickness wounds.

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Silk Proteins

Lamboni

Souho

Osi

et al. 2018

Bioinspired Materials Science and Engineering

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Formulation and characterization of silk sericin–PVA scaffold crosslinked with genipin

Cited by 136 publications

References 47 publications

The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds

The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds

Accelerated Healing of Full-Thickness Wounds by Genipin-Crosslinked Silk Sericin/PVA Scaffolds

Silk Proteins

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