Formulation and characterization of hydroxyapatite‐based composite with enhanced compressive strength and controlled antibiotic release

Abstract

A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid–liquid mixing method. The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths. A synergistic effect of CS and CIP contents on the compressive strength of the CIP‐loaded composite was evidenced. The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone. The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens. On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days. Moreover, in vitro antibacterial tests performed on the biocomposite HA‐CS5‐CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens. According to the showed results, the formulated composite with three‐phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.

Design of antibacterial apatitic composite cement loaded with Ciprofloxacin: Investigations on the physicochemical Properties, release Kinetics, and antibacterial activity

“…Similar findings were reported byAit said et al, (Ait Said et al, 2021), which evaluated the antibacterial activity of hydroxyapatite-chitosan composites loaded with ciprofloxacin (HA-CS-CIP) against the bacteria Staphylococcus aureus and Escherichia coli. The results obtained indicate that the diameters of the zones of inhibition measured for the materials associated with ciprofloxacin are largely superior compared to the reference compounds (HA, CS and HA-CS).…”

Hydroxyapatite, alginate and gelatin composites used for bone regeneration: a systematic review

“…However, it is a fragile material with low mechanical strength (Song et al, 2020;Fitzpatrick et al, 2021). To overcome these limitations, this ceramic has been associated with other types of materials (Liao et al, 2018;Dubey et al, 2021;Ait Said et al, 2021), such as collagen, chitosan, silk, fucoidan, elastin, hyaluronic acid, gelatin and alginate (Bharadwaza & Jayasuriya, 2020). The latter two stand out for their biocompatibility, biodegradability, absence of toxicity and immunogenicity (Meimandi-Parizi et al, 2018;Shi et al, 2019).…”

Development of Chitosan/Mg−Zn−HAP/ZrO2 Bilayer Coating on Ti Alloy for Biomedical Applications

“…A chitosan/M−HAP composite as potential material for biomedical and orthopedic applications. The combination of M−HAP/chitosan composite has good biocompatibility and suitable mechanical properties [53–56] . Deposition of HAP on metallic implants using various coating methods such as electrophoretic deposition, plasma spraying, sol‐gel method, sputter coating, pulsed laser deposition, and physical vapor deposition (PVD) [57,58] .…”