Formononetin, an isoflavone from Astragalus membranaceus inhibits proliferation and metastasis of ovarian cancer cells

Zhang, Jing; Liu, Likun; Wang, Jing; Ren, Baoyin; Zhang, Lin; Li, Weiling

doi:10.1016/j.jep.2018.04.014

Cited by 79 publications

(52 citation statements)

References 31 publications

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“…Compounds 161 , 168, and 290 were obtained and their structure was characterized by NMR spectrometry, which were performed in methanol‐ d 4 on a Bruker Avance III 500 NMR spectrometer ( 1 H: 600 MHz, 13 C:150 MHz; Bruker Corporation, Billerica, MA, USA). Finally, these three compounds were elucidated as daphnoretin, 2H‐1‐benzopryran‐2‐one‐7‐hydroxy and 7‐hydroxy‐3‐(4‐methoxy‐phenyl)‐chromen‐4‐on by comparing with reported compounds (Li, Tong, Cheng, Li, & Yan, ; Zhang et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Structural characterization and anti‐inflammatory activity evaluation of chemical constituents in the extract ofTrifolium repensL.

Chen

Wang

et al. 2019

J Food Biochem

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The chemical constituents in Trifolium repens L. were comprehensively studied by UPLC in this work, and a total number of 308 compounds were detected with 169 ones identified. The possible fragmentation pathways were proposed and fragmentation rules were summarized. On the basis of the concluded strategies, the characterized compounds could be classified into organic acids and their derivatives, alkaloids, amino acids, peptides, flavonoids, oligosaccharides, coumarins, and other types of compounds. This approach provided a rapid way for the identification of constituents in T. repens L., and even in other complex analytes. Among the separation and identification of the constituents, three compounds of great amount were isolated and characterized by NMR. The expression of iNOS and COX‐2 in LPS‐induced RAW 264.7 cells was suppressed by the pretreatment with three isolated constituents. The results implied they may potentially serve as a remedy for the therapy of inflammation. Practical applications This work provided a rapid method for the identification of the complex analyte, which could be used in TCM, natural food and so on. The summarized fragmentation rule could be applied for the analysis of several types of compounds, such as organic acids and their derivatives, alkaloids, amino acids and peptides, flavonoids, oligosaccharides, coumarins, and so on. Most of natural plants contain these kinds of compounds, so these rules could have wide applications. Except the phytochemical investigation, T. repens L. displayed anti‐inflammation activity according to the reported literature, and the three isolated constituents may potentially serve as a remedy for the therapy of inflammation referring to the result of this research.

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Section: Methodsmentioning

confidence: 99%

“…Finally, these three compounds were elucidated as daphnoretin, 2H-1-benzopryran-2-one-7-hydroxy and 7-hydroxy-3-(4-methoxyphenyl)-chromen-4-on by comparing with reported compounds (Li, Tong, Cheng, Li, & Yan, 2014;Zhang et al, 2018).…”

mentioning

confidence: 99%

Structural characterization and anti‐inflammatory activity evaluation of chemical constituents in the extract ofTrifolium repensL.

Chen

Wang

et al. 2019

J Food Biochem

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“…Various molecular compounds (genistein, oleanolic acid, luteolin, kaempferol, and ursolic acid) have been involved in antiinvasive activity [via decreased expression of intercellular adhesion molecule-1 (sICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and matrix metalloproteinases (MMPs)] associated with endometriosis (Kuessel et al, 2017). For example, formononetin, an isoflavone from A. membranaceus, showed potential effects on the induction of apoptosis, and suppression of migration and invasion (Zhang J. et al, 2018).…”

Section: The Antiinvasive Antiadhesion and Angiogenesis Functionsmentioning

confidence: 99%

Modular Characteristics and Mechanism of Action of Herbs for Endometriosis Treatment in Chinese Medicine: A Data Mining and Network Pharmacology–Based Identification

Zheng

et al. 2020

Front. Pharmacol.

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could also regulate VEGF signaling, toll-like reporter signaling, NF-kB signaling, MAPK signaling, PI3K-Akt signaling, and the HIF-1 signaling pathway, among others. Synergies often exist in herb pairs and herbal prescriptions. In conclusion, we identified some important targets, target pairs, and regulatory networks, using bioinformatics and data mining. The combination of data mining and network pharmacology may offer an efficient method for drug discovery and development from herbal medicines.

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“…13 Studies have also shown that formononetin has antitumour effects in several types of cancer, such as breast cancer, osteosarcoma and ovarian cancer. 14,15 Our previous experimental results show that formononetin has a two-way regulatory effect on HUVEC; that is, low concentrations promote proliferation of HUVEC, while high concentrations produce inhibition, 16 but the specific mechanism remains to be understood. After conducting long-term experiments, we found that the solubility of formononetin is low and that the concentration required inhibiting cells is too high.…”

Section: Introductionmentioning

confidence: 98%

Formononetin, J1 and J2 have different effects on endothelial cells via EWSAT1‐TRAF6 and its downstream pathway

Huang

Sui

et al. 2019

J Cellular Molecular Medi

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Formononetin is a natural isoflavone compound found mainly in Chinese herbal medicines such as astragalus and red clover. It is considered to be a typical phytooestrogen. In our previous experiments, it was found that formononetin has a two‐way regulatory effect on endothelial cells (ECs): low concentrations promote the proliferation of ECs and high concentrations have an inhibitory effect. To find a specific mechanism of action and provide a better clinical effect, we performed a structural transformation of formononetin and selected better medicinal properties for formononetin modifier J1 and J2 from a variety of modified constructs. The MTT assay measured the effects of drugs on human umbilical vein endothelial cell (HUVEC) activity. Scratch and transwell experiments validated the effects of the drugs on HUVEC migration and invasion. An in vivo assessment effect of the drugs on ovariectomized rats. Long‐chain non‐coding RNA for EWSAT1, which is abnormally highly expressed in HUVEC, was screened by gene chip, and the effect of the drug on its expression was detected by PCR after the drug was applied. The downstream factors and their pathways were analysed, and the changes in the protein levels after drug treatment were evaluated by Western blot. In conclusion, the mechanism of action of formononetin, J1 and J2 on ECs may be through EWSAT1‐TRAF6 and its downstream pathways.

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Formononetin, an isoflavone from Astragalus membranaceus inhibits proliferation and metastasis of ovarian cancer cells

Cited by 79 publications

References 31 publications

Structural characterization and anti‐inflammatory activity evaluation of chemical constituents in the extract ofTrifolium repensL.

Structural characterization and anti‐inflammatory activity evaluation of chemical constituents in the extract ofTrifolium repensL.

Modular Characteristics and Mechanism of Action of Herbs for Endometriosis Treatment in Chinese Medicine: A Data Mining and Network Pharmacology–Based Identification

Formononetin, J1 and J2 have different effects on endothelial cells via EWSAT1‐TRAF6 and its downstream pathway

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