Formation of lipid reserves in fat body and eggs of the yellow fever mosquito, Aedes aegypti

Ziegler, Rolf; Ibrahim, Mohab

doi:10.1016/s0022-1910(00)00158-x

Cited by 117 publications

(102 citation statements)

References 14 publications

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“…Females with reduced nutritional reserves are well known to produce fewer eggs (Foster, 1995;Ziegler and Ibrahim, 2001;Harrington et al, 2001;Zhou et al, 2004). Females allowed to feed on sugar prior to blood feeding have significantly higher lipid reserves, allowing these females to produce more eggs that contain more lipids (Ziegler and Ibrahim, 2001;Zhou et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Benoit

Patrick

Desai

et al. 2010

Journal of Experimental Biology

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SUMMARYIn this study of the mosquito, Culex pipiens, we examined the impact of multiple bouts of dehydration and rehydration on survival, depletion of metabolic reserves and egg production in both non-diapausing and diapausing females. Mosquitoes provided with access to sugar during rehydration survived longer than those allowed to rehydrate without sugar, and their survival was similar to that of mosquitoes of the same age that were not dehydrated. Among mosquitoes not provided with sugar, each dehydration bout reduced the mosquito's dry mass -an effect likely to be due to the utilization of carbohydrates and lipid reserves. The toll on glycogen and lipid reserves is likely to be especially costly for diapausing mosquitoes that are dependent on these stored reserves for winter survival. Egg production in both non-diapausing and post-diapausing C. pipiens was also reduced in response to multiple bouts of dehydration. Although egg quality was not compromised, the number of eggs produced was reduced. Both non-diapausing and diapausing females can compensate for the nutrient loss due to dehydration by sugar feeding but the opportunity to feed on sugar is likely to be rarely available in the overwintering habitat of diapausing females, thus the impact of dehydration may be especially pronounced in overwintering populations of C. pipiens.

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Section: Discussionmentioning

confidence: 99%

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Benoit

Patrick

Desai

et al. 2010

Journal of Experimental Biology

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“…This small glycogen content in the fat body of R. prolixus may be because it does not ingest large amounts of carbohydrates. In females of Aedes aegypti and Aedes sollicitans, energy reserves accumulate according to the type of meal they imbibe, their age when they feed on blood, and the time they are examined after the blood meal (Briegel, 1990;Ziegler and Ibrahim, 2001). Naksathit et al (1999) showed that mosquitoes fed on sugar contained more glycogen than those fed only on blood, and Zhou et al (2004) demonstrated in Aedes aegypti females that gluconeogenesis from amino acids was rapidly activated after a blood meal.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and mobilization of glycogen and trehalose in adult male Rhodnius prolixus

Mariano

Santos

Gonzalez

et al. 2009

Arch Insect Biochem Physiol

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The vector of Chagas' disease, Rhodnius prolixus, feeds exclusively on blood. The blood meals are slowly digested, and these insects wait some weeks before the next meal. During the life of an insect, energy-requiring processes such as moulting, adult gonadal and reproductive growth, vitellogenesis, muscular activity, and fasting, lead to increased metabolism. Carbohydrates are a major source of energy and their mobilization is important. We determined the amounts of glycogen, trehalose, and glucose present in the fat body and/or hemolymph of adult males of R. prolixus and recorded the processes of accumulation and mobilization of these carbohydrates. We also tested our hypothesis that these processes are under endocrine control. The amount of glycogen in the fat body progressively increased until the fourth day after feeding (from 9.3+/-2.2 to 77. 3+/-7.5 microg/fat body), then declined to values around 36.3+/-4.9 microg/fat body on the fifteenth day after the blood meal. Glycogen synthesis was eliminated in decapitated insects and head-transplanted insects synthesized glycogen. The amount of trehalose in the fat body increased until the sixth day after feeding (from 16. 6+/-1.7 to 40. 6+/-5.3 nmol/fat body), decreased abruptly, and stabilized between days 7 and 15 at values ranging around 15-19 nmol/fat body. Decapitated insects did not synthesize trehalose after feeding, and this effect was reversed in head-transplanted insects. The concentration of trehalose in the hemolymph increased after the blood meal until the third day (from 0.07+/-0.01 to 0.75+/-0.05 mM) and at the fourth day it decreased until the ninth day (0.21+/-0.01 mM), when it increased again until the fourteenth day (0.79+/-0.06 mM) after the blood meal, and then declined again. In decapitated insects, trehalose concentrations did not increase soon after the blood meal and at the third day it was very low, but on the fourteenth day it was close to the control values. The concentration of glucose in the hemolymph of untreated insects remained low and constant (0.18+/-0.01 mM) during the 15 days after feeding, but in decapitated insects it progressively increased until the fifteenth day (2.00+/-0.10 mM). We recorded the highest trehalase activity in midgut, which was maximal at the eighth day after feeding (2,830+/-320 nmol of glucose/organ/h). We infer that in Rhodnius prolixus, the metabolism of glycogen, glucose, and trehalose are controlled by factors from the brain, according to physiological demands at different days after the blood meal.

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“…High acetate incorporation into fat body lipids that precedes the onset of vitellogenic oocyte growth has also been shown for locusts (Gokuldas et al, 1988;Lee and Goldsworthy, 1995) and mosquitoes (Ziegler, 1997). Mosquitoes are capable of converting dietary sugars into lipid reserves during the first days of adult life that are later used to support egg production (Van Handel, 1965;Ziegler and Ibrahim, 2001). Lipid reserves can also be built up to support flight.…”

Section: Discussionmentioning

confidence: 99%

Changes in the biochemical composition of fat body stores during adult development of female crickets, Gryllus bimaculatus

Lorenz

Anand

2004

Arch Insect Biochem Physiol

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Age-dependent changes in the fat body composition and aspects of lipogenesis in the free abdominal fat body of female crickets, Gryllus bimaculatus, were studied. Lipid, protein, glycogen, and free carbohydrate content of the fat body, and fat body wet weight increased simultaneously and sharply from day 0 onwards and were doubled/almost doubled by day 2 after adult emergence. Lipogenic activity of the fat body, fat body weight, and the energy stores in the fat body peaked on day 2, except for free carbohydrate, which peaked on day 3. On day 2, the fat body was mainly comprised of lipid (53.8%) and protein (6.6%), while glycogen and free carbohydrate together contributed less than 1% of the fat body wet weight. After peaking, both lipogenesis and energy stores decreased in a synchronous manner. The depletion of the fat body energy stores and the consequent decrease in the fat body weight were concomitant with a fast and massive gain in ovary weight (day 2: 19.5 +/- 1.5 mg; day 4: 332.8 +/- 31.5 mg) due to the vitellogenic oocyte growth that started on day 2. Our data clearly underline the importance of the free abdominal fat body as a source of energy for reproduction in the cricket. Fat body fatty acid synthase activity coincided with lipogenic activity. Adipokinetic hormone inhibits lipid synthesis in the fat body, but treatment of the fat body with adipokinetic hormone in vitro showed no consistent effect on fatty acid synthase activity.

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Formation of lipid reserves in fat body and eggs of the yellow fever mosquito, Aedes aegypti

Cited by 117 publications

References 14 publications

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Synthesis and mobilization of glycogen and trehalose in adult male Rhodnius prolixus

Changes in the biochemical composition of fat body stores during adult development of female crickets, Gryllus bimaculatus

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