2007
DOI: 10.1529/biophysj.107.107714
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Abstract: To better understand how ceramide modulates the biophysical properties of the membrane, the interactions between palmitoyl-ceramide (PCer) and palmitoyl-sphingomyelin (PSM) were studied in the presence of the fluid phospholipid palmitoyl-oleoyl-phosphatidylcholine (POPC) in membrane model systems. The use of two fluorescent membrane probes distinctly sensitive to lipid phases allowed a thorough biophysical characterization of the ternary system. In these mixtures, PCer recruits POPC and PSM in the fluid phase … Show more

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Cited by 116 publications
(169 citation statements)
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References 43 publications
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“…In this study, we investigate the effect of generating Cer in human embryonic kidney (HEK) cells overexpressing different CerSs, i.e., having distinct SL acyl chain compositions. Using a combination of fl uorescent probes that display different phase-related properties ( 22 ), we show that the biophysical properties of the plasma and intracellular membranes change with the SL acyl chain composition of the cells. Moreover, Cer formation promotes extensive membrane remodeling in a manner that correlates with the acyl chain composition of the Cer that is generated.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In this study, we investigate the effect of generating Cer in human embryonic kidney (HEK) cells overexpressing different CerSs, i.e., having distinct SL acyl chain compositions. Using a combination of fl uorescent probes that display different phase-related properties ( 22 ), we show that the biophysical properties of the plasma and intracellular membranes change with the SL acyl chain composition of the cells. Moreover, Cer formation promotes extensive membrane remodeling in a manner that correlates with the acyl chain composition of the Cer that is generated.…”
Section: Discussionmentioning
confidence: 99%
“…Each of these probes presented different phase-related properties and preferential partition into distinct phases ( 20,22,30,31 ), allowing the identifi cation and characterization of those phases. In addition, some of the probes were expected to localize predominantly in the outer leafl et of the plasma membrane (PM) (such as, NBD-DPPE, TMA-DPH, and Rho-DOPE), whereas others this probe will be localized not only in the PM but also in intracellular membranes.…”
Section: Infl Uence Of Sl Acyl Chain Structure On Membrane Biophysicamentioning
confidence: 99%
“…22 Heerklotz and co-workers used calorimetry to compare the interactions of cholesterol with large unilamellar vesicles of POPC plus 30% cholesterol, sphingomyelin plus 30% cholesterol, and 1:1 sphingomyelin:POPC plus 30% cholesterol. 20 They find that sphingomyelin-cholesterol interactions are favored by 7 kJ/m in sphingomyelin-cholesterol mixtures and by 13 kJ/m in 1:1 sphingomyelin-POPC mixtures over pure POPC-cholesterol mixtures.…”
Section: Introductionmentioning
confidence: 99%
“…Zhao et al conclude that POPC mixtures are close to phase separation sufficiently critical in their thermodynamical state that phase separation can be induced by a "perturbation" such as increased pressure (as in Rappolt et al 14 ), reduced temperature, or perhaps by membrane proteins. Castro et al 22 use multiple flourescent probes to study ternary mixtures of POPC, sphingomyelin, and ceramide. The latter lipid acts very similarly to cholesterol to order lipid bilayers, at least at low concentrations.…”
Section: Introductionmentioning
confidence: 99%
“…As a consequence, such Cers form segregated solid-like condensed domains, in both bilayers and monolayers, that even exclude fluorescent probes and proteins (Carrer and Maggio 1999;Holopainen et al 2001;Härtel et al 2005;Castro et al 2007;Chiantia et al 2008;Busto et al 2009;Karttunen et al 2009;López-Montero et al 2010). Natural mixtures of Cer are mainly composed of long chain Cers.…”
Section: Ceramide Mixturesmentioning
confidence: 99%