2002
DOI: 10.1007/s00726-001-0125-3
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Abstract: Using RT-PCR, the present study investigated the effects of formalin administration on mRNA expression coding for NMDA receptor (NR) subunits and splice variants in rat lumbar spinal cord. Subsequent to formalin injection (5%; subcutaneously) into the hind paw of Sprague-Dawley rats, the animals exhibited the typical biphasic behavioural pain response. Spinal cord (L3-6) was prepared six hours after formalin injection. In controls, NR1-b predominated over NR1-a, and NR1-2 and NR1-4 exceeded over NR1-1 and NR1-… Show more

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Cited by 39 publications
(32 citation statements)
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“…Interestingly, the expression of NR1 and NR2B was increased in the hippocampus of the formaldehyde-treated groups after 7 days of formaldehyde treatment (Fig. 6e-h), consistent with a previous study indicating that acute formaldehyde treatment increases NR1 and NR2B expression (Gaunitz et al 2002). We speculate that the increase in NR1/NR2B expression on day 7 compensates for the non-specific blocking of the NMDA receptor by excess formaldehyde.…”
Section: Discussionsupporting
confidence: 91%
“…Interestingly, the expression of NR1 and NR2B was increased in the hippocampus of the formaldehyde-treated groups after 7 days of formaldehyde treatment (Fig. 6e-h), consistent with a previous study indicating that acute formaldehyde treatment increases NR1 and NR2B expression (Gaunitz et al 2002). We speculate that the increase in NR1/NR2B expression on day 7 compensates for the non-specific blocking of the NMDA receptor by excess formaldehyde.…”
Section: Discussionsupporting
confidence: 91%
“…For example, mRNA and protein levels of the NR2A subunit are up-regulated to a greater extent than NR2B mRNA in the rostral ventromedial medulla (RVM) after carrageenan injection in the hindpaw of rats (Miki et al, 2002). In another study, formalin injected into the paw increased NR2A mRNA expression in the spinal cord (Gaunitz et al, 2002). However, up-regulation of NR2A mRNA might be specific for inflammatory pain states since nerve injury decreased NR2A in the spinal cord (Karlsson et al, 2002).…”
Section: Discussionmentioning
confidence: 97%
“…The reason for this seeming change in presynaptic NMDAR function in response to previous opiate exposure is not evident. Studies are underway examining the role of possible mechanisms that might alter the physiological effects of NMDA application, including differential NMDA subunit expression (Yamakura and Shimoji, 1999;Gaunitz et al, 2002;Martin et al, 2004) and/or receptor phosphorylation MacDonald et al, 2001;Pan, 2003;Smith et al, 2003;Kalia et al, 2004).…”
Section: Discussionmentioning
confidence: 99%