Food allergy/hypersensitivity: Antigenicity or timing?

Paschoal, Patrícia Olaya; Campos, Sylvia Maria Nicolau; Pedruzzi, Monique M.B.; Garrido, Valéria; Bisso, Mônica; Antunes, Danielle Mota Fontes; Nóbrega, Alberto; Teixeira, Gerlinde Agate Platais Brasil

doi:10.1016/j.imbio.2008.09.007

Cited by 14 publications

(11 citation statements)

References 25 publications

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“…In this work, along with the assessment of clinical aspects during the induction of food allergy and gut inflammation, gut histopathology was performed. Similar to previous findings of our group, the results obtained led to an array of histological alterations spanning from normal histology (control animals) to severe inflammation of the duodenum (allergic group) . The pathophysiological changes induced by the inflammatory response led to alterations both in the cell count and in the villus architecture.…”

Section: Discussionsupporting

confidence: 87%

“…Similar to previous findings of our group, the results obtained led to an array of histological alterations spanning from normal histology (control animals) to severe inflammation of the duodenum (allergic group). 19,40,41 The pathophysiological changes induced by the inflammatory response led to alterations both in the cell count and in the villus architecture. No animal presented classical anaphylaxis symptoms, such as convulsions, wheezing, laboured respiration or pilar erecti.…”

Section: Discussionmentioning

confidence: 99%

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A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Silva

Soares

Mattos

et al. 2018

Int J Experimental Path

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Gut-associated intestinal lymphoid tissue, the largest secondary lymphoid organ in the human body, constantly samples antigens from the gut lumen, presenting as a default response the activation of TCD4 FOXP3 regulatory T cells that secrete a profile of anti-inflammatory cytokines maintaining gut homeostasis denominated from an immunological perspective as mucosal tolerance. However, when antigens are sampled in an inflammatory setting, the immune response may either be protective, in the case of harmful pathogens, or cause further inflammatory reactions as in food allergy, inflammatory bowel diseases, coeliac disease or food protein-induced enterocolitis syndrome. Therefore, there is a need for accurate and consistent experimental models. However, a drawback in comparing these models is the lack of a classification system similar to that which is already used for humans. Thus, the aim of this work was to propose a classification system of the small intestinal histomorphology in experimental mice. To do this we used a mouse antigen-specific gut inflammation model developed by our research group. Duodenum sections stained with haematoxylin-eosin and Alcian blue were scanned using the APERIO scanning system and analysed with the ImageScope software. The evaluated parameters were villus area, villus height and width, enterocyte count, mononuclear intra-epithelial leucocyte and goblet cell counts, and architectural and cellular ratios. Food-sensitized animals challenged with a diet containing the corresponding food allergen presented, as for humans, time-dependent shortened and widened villi accompanied by leucocyte infiltrate and loss of goblet cells. With these data, we were able to establish a classification system for experimental intestinal inflammation in mice thus permitting better comparisons among and between experiments than has been possible previously.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Silva

Soares

Mattos

et al. 2018

Int J Experimental Path

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“…The immunological status of the gut, as has been previously demonstrated by our group, is essential to induce either allergy or tolerance after the initial contact of the food with the mucosa. [26] Our data agree with those of the literature [27,28] regarding the pH of the buffer used in the extraction of peanut proteins -neutral pH is more efficient.…”

Section: Immunogenicity/immunoreactivitysupporting

confidence: 92%

“…Only to site a few of the potential influencers of allergenicity are: the food matrix [23], protein stability during processing [25], and the immunological status of the entry port [26]. Food matrix can be defined as the way the chemical components of food (protein, fatty acids, carbohydrates, and micronutrients) interact to establish the structural organization from microscopic to macroscopic scales.…”

Section: Immunogenicity/immunoreactivitymentioning

confidence: 99%

Allergen Extraction: Factors Influencing the Yield, Allergenicity and Sensitivity of Immunoassays

Jra

et al. 2020

Preprint

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19Food allergy is an IgE and or IgG immune-mediated reaction to food antigens.20 Knowledge of the allergenicity properties of proteins, how they react in the body and 21 in diagnostic tests is necessary to adequately assess the allergenic potential of both 22 natural foods and those produced through biotechnological processes. Thus, our aim 23 was analyze the factors those influence the protein extraction of foods in terms of 24 yield, allergenicity and sensitivity in immunoassays. Peanut proteins were extracted 25 using 4 distinct extraction buffers (physiological saline, tris buffer, borate buffer with 26 and without β-mercaptoethanol). The protein concentration of the obtained extracts 27 was determined by the Lowry method. Polyacrylamide electrophoresis (SDS-PAGE) 28 was used to compare the protein profile of each extract. Immunogenicity of each 29 extract was verified by sensitizing two mouse strains (Balb/c and C57/BL6) with 30 100μg of the proteins, and the immunoreactivity was determined by ELISA. Our 31 results show that extraction with the distinct buffers resulted in protein solutions with 32 different yields and profiles. The antigenicity of the different extracts in a murine 33 model of systemic immunization also demonstrated distinct patterns that varied 34 depending on the extraction methods and mouse strain. The immunoreactivity varied 35 in accordance to the protein extract used to coat the microtitration plates. In 36 conclusion, the protein yield and profile in the extracts is critically influenced by the 37 salt composition and pH of the extraction buffers. This in turn influences both in vivo 38 immunogenicity and in vitro immunoreactivity. 39 Introduction 40 Considering that 4% to 8% of children present allergies and that a significant 41 portion of these present symptoms that prevent school attendance which in turn takes, 42 at least one of their caregivers away from work we can say that allergies have a high 43 social impact [1]. In addition to absence at work, altering meal preparation routine 44 and other social activities of the family negatively impacts stress levels [2]. Thus, 45 adequate diagnosis is fundamental. 46 The best-known measure to prevent food allergy symptomatology in allergic 47 people is the adoption of exclusion diets. To ensure clearer food labeling many 48 countries have food allergen labeling laws. For example, the Food Allergen Labeling 49 and Consumer Protection Act (FALCPA/USA-2004) requires that foods are labeled to 3 50 identify the eight major food allergens: milk, egg, fish, crustacean-shellfish, tree nuts, 51 wheat, peanuts and soybeans which account for over 90% of all documented food 52 allergies in the U.S. [3]. Along with these 8 the Australian law requires the labeling of 53 two other foods -sesame and lupin [4]. In the European Union 14 foods are on the 54 required labeling list: the ten previously cited plus celery, mustard, Sulphur dioxide, 55 and mollusks. 56 Regardless of whether ingested food is derived from animals or plants, the 57 food matrix i...

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“…Previous studies from our group reported the inflammatory milieu of the gut of allergic animals submitted to oral challenge, as well as the normal millieu of the gut of allergic animals that were not submitted to oral challenge (23,24). The analysis performed in these papers used either OM or DM; however, no comparison was performed.…”

Section: Discussionmentioning

confidence: 99%

Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model

et al. 2017

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Abstract. Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niterói, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research. Thus, a mouse gut inflammation model developed by our research group was used in the present study. Analysis was performed using optical microscopy and digital imaging using the APERIO scanning system and the ImageScope ® software. Intestinal epithelial cells (IECs), intra epithelial leucocytes (IEL), and villi number and area were evaluated. No significant differences were observed in villi number, IEC and IEL; however, the villi area was significantly smaller when measured using the computer. Thus, the present study indicates that virtual microscopy is a trustworthy method for research purposes.

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Food allergy/hypersensitivity: Antigenicity or timing?

Cited by 14 publications

References 25 publications

A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Allergen Extraction: Factors Influencing the Yield, Allergenicity and Sensitivity of Immunoassays

Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model

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