Follicular fluid concentrations of interleukin-12 and interleukin-8 in IVF cycles

Gazvani, M. R.; Bates, Michelle; Vince, Gillian S.; Christmas, Stephen E.; Lewis‐Jones, D. I.; Kingsland, Charles

doi:10.1016/s0015-0282(00)01538-7

Cited by 49 publications

(49 citation statements)

References 20 publications

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“…IL-8 was suggested to be an essential part of folliculogenesis. In a previous study, FF IL-8 concentrations were compared between pregnant (n=11) and non-pregnant (n=33) women following IVF and were found to be no different (8). Our results are in accordance with them.…”

Section: Student's T-testsupporting

confidence: 90%

“…FF IL-6 concentrations were noted to be significantly higher in pregnant women (6). No correlation was found between FF IL-8 concentrations and fertilization or implantation rates (8). FF IL-12 concentrations were reported to be associated with a negative outcome in IVF treatment (6,8).…”

Section: Introductionmentioning

confidence: 93%

“…No correlation was found between FF IL-8 concentrations and fertilization or implantation rates (8). FF IL-12 concentrations were reported to be associated with a negative outcome in IVF treatment (6,8). On the contrary, some researchers found no significant difference in FF IL-12 concentrations between pregnant and nonpregnant patients (5,7).…”

Section: Introductionmentioning

confidence: 97%

“…Some authors suggested increased (4), some suggested decreased (5) and some suggested no different (6) FF IL-1β concentrations in pregnant women compared to nonpregnant ones. FF IL-6, IL-8 and IL-12 levels were also investigated in IVF cycles (6)(7)(8). FF IL-6 concentrations were noted to be significantly higher in pregnant women (6).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome

Kumbak

Akçin

Attar

et al. 2009

Fertility and Sterility

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Objective:The role of cytokines and nitric oxide (NO) in ovarian folliculogenesis and the development of mature and fertilizable oocytes is controversial. The aim of this study is to determine the concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α and NO in the follicular fluid (FF) and blood serum (S) of patients undergoing assisted reproductive treatment (ART) and to investigate whether these cytokines could be used as a predictive parameter for ART outcome. Material and Methods:A retrospective clinical study was performed at a university hospital including a total of 85 women who underwent ART. FF and serum samples were collected at the time of oocyte retrieval and measured for interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α by the enzyme-linked immunosorbant assay (ELISA) technique, using commercially available kits and NO by the nitrate/nitrite colorimetric assay. The results were compared between the women who became pregnant and those who did not following ART.Results: No significant difference was found in the FF and blood serum concentrations of the cytokines and NO between pregnant and non-pregnant women. Conclusion: Abstract ÖzetOriginal Investigation 132

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Section: Student's T-testsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome

Kumbak

Akçin

Attar

et al. 2009

Fertility and Sterility

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show abstract

“…A positive association of IL-6 with oocyte maturation [31] and IL-1β with embryo development and pregnancy [32] is documented. A majority of findings have indicated that IL-12 is negatively associated with folliculogenesis, oocyte quality, and implantation [30,33,34]. TNF-α, reported to be significantly higher in follicular fluids corresponding to poor-quality oocytes and embryos, possibly deteriorates the microenvironment in the follicle [35,36].…”

Section: Discussionmentioning

confidence: 99%

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Singh

Dutta

Chattopadhyay

et al. 2016

J Assist Reprod Genet

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Purpose The study aimed to investigate key intrafollicular prognostic factors among various cytokines and angiogenic molecules for prediction of mature oocytes and good-quality embryos in women with endometriosis undergoing in vitro fertilization (IVF). Methods Paired follicular fluid and serum samples were collected from 200 women with advanced stage endometriosis and 140 normal ovulating women during oocyte retrieval. The concentrations of cytokines (pro-inflammatory: IL-1β, TNF-α, IL-2, IL-8, IL-12, IFN-γ; anti-inflammatory: IL-4, IL-6, IL-10) and angiogenic molecules (vascular endothelial growth factor (VEGF), adrenomedullin, angiogenin) were determined in follicular fluid and serum using ELISA. Expression of these molecules was subjected to multivariate analysis for the identification of major predictive markers of oocyte and embryo quality. Receiver operating characteristic (ROC) curve was applied to determine the best cutoff point for the discrimination between mature and immature oocytes in these women. Results Significant increases in levels of cytokines and angiogenic molecules were observed in women with endometriosis compared to controls (P < 0.001). From the validated partial least squares-discriminant analysis (PLS-DA) model, IL-8, IL-12, and adrenomedullin were identified as the most important factors contributing to endometriosis and were negatively associated with oocyte maturity and embryo quality. Conclusion The levels of IL-8, IL-12, and adrenomedullin may be good indicators of embryo and oocyte quality in endometriosis patients undergoing IVF. Further studies are necessary to ascertain the potential of these markers for oocyte and embryo developmental competence which may help improve the chances of a successful IVF in endometriosis patients.

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Diet‐induced obesity alters immune cell infiltration and expression of inflammatory cytokine genes in mouse ovarian and peri‐ovarian adipose depot tissues

Nteeba

Ortinau

Perfield

et al. 2013

Molecular Reproduction Devel

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Dysregulation of immune cells and/or altered inflammatory signaling have been implicated with reproductive dysfunction. Physiological changes leading to perturbations in the profile of immune cells and/or pro-inflammatory cytokines in or around female reproductive tissue could potentially have profound effects on ovarian function. Obesity is associated with chronic low-grade inflammation due, in part, to increased immune cell infiltration and inflammation in visceral adipose depots. This study investigated the impact of diet-induced obesity on immune cell infiltration and inflammation in peri-ovarian adipose tissue and mRNA expression of key inflammatory markers and microRNAs (miRs) in ovarian tissue. Six-week-old female C57Bl/6J mice were fed a standard chow or high-fat diet (HFD; 60% kcal fat) for approximately 7 months, at which time peri-ovarian adipose tissue and ovarian tissues were collected. Histological analysis of peri-ovarian adipose tissue from obese mice revealed increased (P < 0.05) adipocyte size and the presence of crown-like structures, the morphological presentation of infiltrating immune cells in adipose tissue, along with increases (P < 0.05) in the mRNA levels of markers of T-cells, activated macrophages, inflammatory cytokines, and chemokines. Ovarian mRNA levels of Il1b, Il6, Tnfa, p55, p75, Ccl2, Ikbkb, and Rela were higher in obese tissue (P < 0.05), with a strong trend (P = 0.06) for an increase in Nos2 and RELA protein. Additionally, ovarian miR125b and miR143 levels were decreased (P = 0.1). These data demonstrate that diet-induced obesity elevates expression of inflammatory-mediator genes in both the ovary and surrounding adipose depot, potentially negatively affecting ovarian function.

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Follicular fluid concentrations of interleukin-12 and interleukin-8 in IVF cycles

Cited by 49 publications

References 20 publications

Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome

Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Diet‐induced obesity alters immune cell infiltration and expression of inflammatory cytokine genes in mouse ovarian and peri‐ovarian adipose depot tissues

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