Follicular fluid antibodies to Chlamydia trachomatis and human heat shock protein-60 kDa and infertility in women

Cortiñas, Paula; Muñoz, María Gladys; Loureiro, Carmen L.; Pujol, Flor H.

doi:10.1016/j.arcmed.2003.09.014

Cited by 15 publications

(10 citation statements)

References 31 publications

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“…Viable C. trachomatis can also persist in macrophages, and this may provide an alternative target cell for chlamydial replication in a number of infections; follicular fluid contains tissue macrophages in large amounts (14). Other authors suggested that in these patients the poor IVF outcome was associated with subclinical chlamydial infection (19)(20)(21)(22)(23)(24)(25)(26).…”

mentioning

confidence: 95%

Autoimmune response to Chlamydia trachomatis infection and in vitro fertilization outcome

Pacchiarotti

Sbracia

Mohamed

et al. 2009

Fertility and Sterility

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mentioning

confidence: 95%

Autoimmune response to Chlamydia trachomatis infection and in vitro fertilization outcome

Pacchiarotti

Sbracia

Mohamed

et al. 2009

Fertility and Sterility

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“…It has been shown that cellular responses with high interferon-g (IFN-g) production, like T-helper 1 (Th1) delayed type hypersensitivity (DTH) reactions, are protective against chlamydial infections, but Th2 DTH itself can elicit chronic pathologies [7,8]. Other studies have revealed the important role played by chlamydial 60-kDa heat shock protein (Hsp60) in tissue damage during chronic infection [9,10]. Hsp60 belongs to a family of related proteins which have been conserved during evolution.…”

Section: Introductionmentioning

confidence: 99%

Sustained Interleukin‐6 and Interleukin‐8 Expression Following Infection with Chlamydia trachomatis Serovar L2 in a HeLa/THP‐1 Cell Co‐culture Model

et al. 2006

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Correspondence to: Dr M. Ravaoarinoro, Département de microbiologie médicale et infectiologie, section de microbiologie clinique, CHUM-Hôtel-Dieu, 3840 rue St-Urbain, Porte 5-206, Montréal, QC H2W 1T8, Canada. E-mail: madeleine.ravaoarinoro.chum@ssss.gouv.qc.ca AbstractChlamydia trachomatis, an intracellular obligate bacterium, remains responsible for a large spectrum of disorders that can progress to chronic diseases, resulting in severe sequelae, such as tubal infertility and blindness. These sequelae may be due to deleterious immune responses induced by repeated or persistent infections. By initiating and regulating inflammation as well as immune responses, pro-inflammatory cytokines secreted by local infected epithelial and immune cells, such as monocytes, may play an essential role in immunity and in the immunopathogenesis of chlamydial diseases. In this study, we mimicked the in vivo interaction between epithelial cells and monocytes by co-culturing epithelial-like HeLa cells with monocyte-like THP-1 cells. Pro-inflammatory cytokines [interleukin-b (IL-1b), IL-6, IL-8, IL-10, IL-12p70 and tumour necrosis factora (TNF-a)] were measured by multiplexed cytometric bead array assay over a period of 18 days. We observed that pro-inflammatory cytokine secretion was augmented after C. trachomatis infection in HeLa and THP-1 cells. However, this heightened secretion was subsequently reduced. When infected HeLa cells were co-cultured with THP-1 cells, IL-6 and IL-8 secretion was sustained, IL-1b expression followed a bell-shaped curve and IL-10, IL-12p70 and TNF-a synthesis was downregulated. IL-6 and IL-8 may be involved in the immunopathogenesis of chronic chlamydial infections. We also observed that throughout C. trachomatis persistence induced by doxycycline (Dox) treatment, IL-1b, IL-6, IL-8 and TNF-a expression was reduced, whereas the synthesis of IL-10 and IL12p70 remained unchanged but not sustained. Thus, during chlamydial persistence infection evoked by treatment with Dox, none of the tested cytokines showed sustained expression.

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“…Immunity to the cHSP60 is more typically associated with a chronic upper gential tract infection and fallopian tube damage than with an acute infection of the lower DFA: direct fluoresence assay, PCR: polymerase chain reaction, MOMP: major outer membrane protein, cHSP: Chlamydia heat shock protein, Group 1A: primary infertility, Group 1B: secondary infertility, Group 2A: discharge, Group 2B: cervicitis, Sens: sensitivity, Specif: specificity, PPV: positive predictive value, NPV: negative predictive value genital tract suggesting that chlamydial HSP60 plays an important role in the immunopathogenesis of C. trachomatis infection [13][14][15][16][17]. Chronic C. trachomatis upper genital tract infection or reinfections may incite an autoimmune response through molecular mimicry in host that may lead to tissue damage as chlamydial growth is inhibited by the acquired immune response [2].…”

Section: Discussionmentioning

confidence: 99%

Chlamydia trachomatis-Specific Heat Shock Proteins 60 Antibodies can Serve as Prognostic Marker in Secondary Infertile Women

et al. 2008

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Follicular fluid antibodies to Chlamydia trachomatis and human heat shock protein-60 kDa and infertility in women

Cited by 15 publications

References 31 publications

Autoimmune response to Chlamydia trachomatis infection and in vitro fertilization outcome

Autoimmune response to Chlamydia trachomatis infection and in vitro fertilization outcome

Sustained Interleukin‐6 and Interleukin‐8 Expression Following Infection with Chlamydia trachomatis Serovar L2 in a HeLa/THP‐1 Cell Co‐culture Model

Chlamydia trachomatis-Specific Heat Shock Proteins 60 Antibodies can Serve as Prognostic Marker in Secondary Infertile Women

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