Foliar storage and extraction methods for quantitative analysis of piperidine alkaloids from ponderosa pine (Pinus ponderosa)

Genetic variation of piperidine alkaloids in Pinus ponderosa: a common garden study

“…Of 501 seedlings sampled, every one had measurable quantities of piperidine alkaloids. Alkaloid-free ponderosa pine trees have been discovered in the Deschutes region (Gerson and Kelsey, 1998), but the absence was attributable, at least partially, to nitrogen limitations (Gerson and Kelsey, 1999b). None of the Deschutes families in the present study lacked alkaloids, further suggesting that those alkaloid-free trees were exhibiting environmental rather than genetic constraints.…”

Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

“…(Chinese name Zhe Beimu) being the first one from genus Fritillaria [4]. Alkaloids, as the main active ingredients, contribute to the antitussive and expectorant function and they are usually extracted by classical solvent extraction [5,6]. According to Chinese Pharmacopeia (2010 edition), the content of peimine and peiminine in the bulb of F. Thunbergii extracted by the CHCl 3 /CH 3 OH = 4:1 and analyzed by HPLC must be higher than 0.1% for medical use (chemical structure of peimisine, peimine, and peiminine see Figure 1) [7].…”