1992
DOI: 10.1021/bi00123a008 View full text |Buy / Rent full text
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Abstract: A polyvalent antiserum which precipitates the native, folded, but not the denatured molecular forms of pig intestinal aminopeptidase N (EC 3.4.11.2) and sucrase-isomaltase (EC 3.2.1.48, EC 3.2.1.10) was used to determine the kinetics of polypeptide folding of the two newly synthesized brush border enzymes. In pulse-labeled mucosal explants, complete synthesis of the polypeptide chains of aminopeptidase N and sucrase-isomaltase required about 2 and 4 min, respectively, whereas maximal antiserum precipitation wa… Show more

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“…It has long been assumed that high mannose content, as distinct from complex glycosylation, is required for surface expression [50,51], while dimerization is not believed to be required [47]. Both surface expression and enzymatic activity require correct folding of the extracellular domain of APN [50,52,53]. Furthermore, it was suggested that intramolecular disulfide bridges are involved in defining the tertiary structure of APN [39] and the data presented here strongly support this concept.…”
Section: Discussionsupporting
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“…It has long been assumed that high mannose content, as distinct from complex glycosylation, is required for surface expression [50,51], while dimerization is not believed to be required [47]. Both surface expression and enzymatic activity require correct folding of the extracellular domain of APN [50,52,53]. Furthermore, it was suggested that intramolecular disulfide bridges are involved in defining the tertiary structure of APN [39] and the data presented here strongly support this concept.…”
Section: Discussionsupporting
“…Generalized lack of disaccharidases suggests the N-glycans are important for the transport, stability, or accumulation of these enzymes on the brush border [Danielsen, 1992]. We suggest that children with neurological symptoms and generalized disaccharidase deficiency, manifesting as feeding intolerance, should be tested for CDG.…”
Section: Discussionmentioning
“…We recently published a report on a patient with CDG-Id where hypoglycemia was a leading symptom [Sun et al, 2005], but that was not seen in these children, underscoring the phenotypic variability in this disorder. Generalized lack of disaccharidases suggests the N-glycans are important for the transport, stability, or accumulation of these enzymes on the brush border [Danielsen, 1992]. We suggest that children with neurological symptoms and generalized disaccharidase deficiency, manifesting as feeding intolerance, should be tested for CDG.…”
Section: Discussionmentioning
“…Monoglucose high mannose chains have been implicated in folding of nascent proteins (18) as well as with the chaperone protein calnexin interaction (19) prior to entry into the Golgi. However, the ability of the denatured mature LHR to refold spontaneously during electrophoretic transfer to nitrocellulose in ligand blots (Ref.…”
Section: Discussionmentioning