Fold 2D Woven DNA Origami to Origami⁺ Structures

Abstract

DNA origami strategy has greatly promoted the development of DNA nanotechnology as it can construct delicate 2D/3D nanostructures. The current method to prepare DNA origami is through a woven approach to fix a long strand of DNA into certain shapes. This article describes a novel strategy to fold the same 2D DNA sheet into multiple complex structures driven by introducing hydrophobic interaction. The pathway of the folding process can be adjusted by tuning the distribution and chronic order of cholesterol, resulting in different complicated structures. This method is proven efficient for constructing variable nanostructures based on the same DNA origami sheet, and termed as origami + . This work represents a new strategy in DNA nanotechnology and will improve the ability to manipulate objects at nanoscale.

Enhanced Catalysis from Multienzyme Cascades Assembled on a DNA Origami Triangle

“…Although the triangle can and will flex in solution, it is unlikely that all the DNA structures would assume a fully curled-up conformation where all the enzymes are placed sufficiently proximal to each other to mimic the other closely spaced arrangements. 61 The ∼27-fold enhancement observed for this assembly is even better that the 19-fold seen for the 2:2:2 configuration, where the enzymes are <10 nm from each other, a value where enzyme channeling should be occurring. 30,62 Moreover, the 19-fold enhancement is identical to the 18-fold predicted from just adding the observed enhancement values of each enzyme (Figure 5).…”

Calcium-Differentiated Cellular Internalization of Allosteric Framework Nucleic Acids for Targeted Payload Delivery

“…In AFM images (left column), bright dots represent the homogeneously monodispersed FNA nanovehicle, of which the height (right column) increases from about 2 to 4 nm upon the addition of K + . As we know, most of 3D DNA architectures usually differs from the 2D ones in their height in AFM images. − The height change of the FNA nanovehicle strongly suggests its 2D-to-3D transformation was induced by K + . Moreover, we employed a Cy3/Cy5 FRET pair to monitor the folding process of the FNA nanovehicle (Figure c).…”

Selective In Situ Growth of Programable Noble Metal Nanoclusters on DNA Origami

“…Interestingly, AFM images showed that the DNA origami folded along the helical axis into a tube‐like structure, wrapping the gold nanocluster inside (Figures S29−30). This was possibly due to the nucleation of adjacent Poly‐A sites and the flexibility of the single‐layer rectangular origami along the helices [37, 48–50]. This difference after Au 3+ reduction is obvious compared to the bare origami (Figure 3i), and Au‐DNA probe functionalized origami (Figure 3j).…”