Focal adhesion kinase is involved in the migration of human osteosarcoma cells

Shan, Feng; Shi, Xin; Ren, Ke; Wu, Sujia; Sun, Xin

doi:10.3892/ol.2015.3131

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…Focal adhesion contacts are highly dynamic structures that undergo constant protein mobilization, assembly and disassembly during cell migration and spread and serve as spots for cross‐talk between actin and microtubule cytoskeletal networks . FAK is a signaling molecule that is known to be a key regulator of a number of cell functions, among which are cell spreading, migration, and survival and its signalling is known to be an important regulator of the ECM‐induced osteogenesis . Inhibition of FAK promotes focal adhesion maturation and reduces cell migration .…”

Section: Discussionmentioning

confidence: 99%

“…28 FAK is a signaling molecule that is known to be a key regulator of a number of cell functions, among which are cell spreading, migration, and survival and its signalling is known to be an important regulator of the ECM-induced osteogenesis. 30,31,[49][50][51][52][53] Inhibition of FAK promotes focal adhesion maturation and reduces cell migration. 51 Expectedly, nano-TiO 2 treated cells had a concentration-dependent decrease in the size of focal adhesions irrespective of the NP size.…”

Section: Discussionmentioning

confidence: 99%

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Ibrahim

Schoelermann

Mustafa

et al. 2018

J Biomedical Materials Res

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Human exposure to titanium dioxide nanoparticles (nano-TiO ) is increasing. An internal source of nano-TiO is represented by titanium-based orthopedic and dental implants can release nanoparticles (NPs) upon abrasion. Little is known about how the size of NPs influences their interaction with cytoskeletal protein networks and the functional/homeostatic consequences that might follow at the implant-bone interface with regard to osteoblasts. We investigated the effects of size of anatase nano-TiO on SaOS-2 human osteoblast-like cells exposed to clinically relevant concentrations (0.05, 0.5, 5 mg/L) of 5 and 40 nm spherical nano-TiO . Cell viability and proliferation, adhesion, spread and migration were assessed, as well as the orientation of actin and microtubule cytoskeletal networks. The phosphorylation of focal adhesion kinase (p-FAK ) and the expression of vinculin in response to nano-TiO were also assessed. Treatment with nano-TiO disrupted the actin and microtubule cytoskeletal networks leading to morphological modifications of SaOS-2 cells. The phosphorylation of p-FAK and the expression of vinculin were also modified depending on the particle size, which affected cell adhesion. Consequently, the cell migration was significantly impaired in the 5 nm-exposed cells compared to unexposed cells. The present work shows that the orientation of cytoskeletal networks and the focal adhesion proteins and subsequently the adhesion, spread and migration of SaOS-2 cells were affected by the selected nano-TiO in a size dependent manner. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2582-2593, 2018.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Ibrahim

Schoelermann

Mustafa

et al. 2018

J Biomedical Materials Res

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“…Targeting FAK with the kinase inhibitor PF-562271 and shRNA inhibited Ewing sarcoma cell growth in vitro and in vivo . High expression of FAK in osteosarcoma cells was associated with a stronger migration ability, which could be suppressed by targeting FAK with selective siRNA 60 . The combination of targeting FAK by shRNA with cisplatin showed enhanced apoptosis in osteosarcoma cells 61 .…”

Section: Targeting Other Non-receptor Tyrosine Kinases (Nrtks)mentioning

confidence: 98%

Targeting protein kinases to reverse multidrug resistance in sarcoma

Chen

Shen

Choy

et al. 2016

Cancer Treatment Reviews

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Sarcomas are a group of cancers that arise from transformed cells of mesenchymal origin. They can be classified into over 50 subtypes, accounting for approximately 1% of adult and 15% of pediatric cancers. Wide surgical resection, radiotherapy, and chemotherapy are the most common treatments for the majority of sarcomas. Among these therapies, chemotherapy can palliate symptoms and prolong life for some sarcoma patients. However, sarcoma cells can have intrinsic or acquired resistance after treatment with chemotherapeutics drugs, leading to the development of multidrug resistance (MDR). MDR attenuates the efficacy of anticancer drugs and results in treatment failure for sarcomas. Therefore, overcoming MDR is an unmet need for sarcoma therapy. Certain protein kinases demonstrate aberrant expression and/or activity in sarcoma cells, which have been found to be involved in the regulation of sarcoma cell progression, such as cell cycle, apoptosis, and survival. Inhibiting these protein kinases may not only decrease the proliferation and growth of sarcoma cells, but also reverse their resistance to chemotherapeutic drugs to subsequently reduce the doses of anticancer drugs and decrease drug side-effects. The discovery of novel strategies targeting protein kinases opens a door to a new area of sarcoma research and provides insight into the mechanisms of MDR in chemotherapy. This review will focus on the recent studies in targeting protein kinase to reverse chemotherapeutic drug resistance in sarcoma.

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“…found in human osteosarcoma, and affects the migration and invasion ability of osteosarcoma cells (22). To determine whether CD155 is related to FAK protein levels in osteosarcoma, we performed western blot analysis for FAK and pFAK.…”

Section: Cd155 Inhibits Osteosarcoma Metastasis Through Repressing Famentioning

confidence: 99%

Overexpression of CD155 relates to metastasis and invasion in osteosarcoma

Zhuo

et al. 2018

Oncol Lett

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The rapid development of metastatic lesions remains the leading cause of mortality for patients with osteosarcoma. CD155 serves a key role in cancer cell migration, invasion and metastasis. However, the function and mechanism of CD155 has not been explored in osteosarcoma metastasis. In the present study, we found that CD155 was significantly upregulated in lung metastatic tissue and the highly metastatic cell line K7M2-WT (K7M2) of osteosarcoma. Overexpression of CD155 in K7M2 cells enhanced lung metastasis, while inhibition of CD155 by an anti-CD155 monoclonal antibody reduced metastasis. Blocking of CD155 also decreased migration and invasion of K7M2 cells . A western blot analysis revealed that blocking of CD155 inhibits metastasis by downregulating focal adhesion kinase (FAK) and phosphorylated FAK (pFAK) in osteosarcoma. The results revealed that CD155 serves a crucial role in the metastasis of osteosarcoma by regulating FAK and may provide a novel molecular target for therapeutic intervention in metastatic osteosarcoma.

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Focal adhesion kinase is involved in the migration of human osteosarcoma cells

Cited by 8 publications

References 16 publications

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Targeting protein kinases to reverse multidrug resistance in sarcoma

Overexpression of CD155 relates to metastasis and invasion in osteosarcoma

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Focal adhesion kinase is involved in the migration of human osteosarcoma cells

Cited by 8 publications

References 16 publications

TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Targeting protein kinases to reverse multidrug resistance in sarcoma

Overexpression of CD155 relates to metastasis and invasion in osteosarcoma

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner