Fluxomics – connecting ‘omics analysis and phenotypes

Abstract

Here we outline the principles of the different approaches and their relative advantages. We demonstrate the unique contribution of flux analysis for phenotype elucidation using a thoroughly studied metabolic reaction as a case study, the microbial aerobic/anaerobic shift, highlighting the importance of flux analysis as a single layer of data as well as interlaced in multi-omics studies.

Influence of HEK293 metabolism on the production of viral vectors and vaccine

“…Such methodologies and approaches are presented in Fig. 1 and have been extensively reviewed for HEK293 cells [20][21][22][23][24].…”

Metabolomics and fluxomics in biotechnology: current trends

“…Fluxomics -to be precise, 13 C-Metabolic Flux Analysis ( 13 C-MFA) -is an area of science that has been developed for about twenty years now, and it is based on the utilization of stable, isotope 13 C-labeled substrates used for accurate tracking changes in the distribution of metabolites in biochemical pathways (Stephanopoulos, 1999;Crown and Antoniewicz, 2013a;Winter and Krömer, 2013).…”

Systems Biology of the Fluxome

“…Modeling of metabolic networks can be done based on constraints of mass and charge conservation along with stoichiometric and thermodynamic ones [9,36,37]. Accounting for the stoichiometry of the reactants and products of biochemical reactions, flux balance analysis (FBA) can estimate metabolic fluxes without knowledge about the kinetics of the participating enzymes.…”

“…Although 13 C-fluxomics does not rely on cofactor balances or optimization functions [37] it requires a priori knowledge of possible distributions of the tracer used within the network. Due to metabolic network size restriction, most flux analyses performed with this methodology so far has been limited to the central carbon metabolism [31].…”