Fluorinated Oils as Experimental Vitreous Substitutes

Perfluorocarbon Effects on Rabbit Blood-Retinal Barrier Permeability

“…The present data confirm other observa tions on the absence of toxicity of this com pound on the retina [7][8][9]11 ], as well as simi lar observations of lack of toxicity on the cor neal endothelium [4], The latter observation is of importance because comparisons of other toxic compounds on both corneal endo thelium and blood-retinal barrier have indi cated that the corneal endothelium is the more sensitive paradigm for toxicity evalua tion of such materials [4,[17][18][19][20] vitreous at a substantial volume (25% of the total capacity of the vitreous cavity), offers no overt toxic effects on the blood-retinal bar rier, albeit the rabbit retinal vasculature is not the same as that of a human eye. Prior studies on retinal toxicity of perfluoro-n-octane indi cated that even large quantities of this liquid (80-90% replacement of vitreous volume) caused no change in the electroretinogram, although some histopathological changes were indicated where the liquid contacted the retina [11],…”

Perfluorocarbon Effects on Rabbit Blood-Retinal Barrier Permeability

“…The present data confirm other observa tions on the absence of toxicity of this com pound on the retina [7][8][9]11 ], as well as simi lar observations of lack of toxicity on the cor neal endothelium [4], The latter observation is of importance because comparisons of other toxic compounds on both corneal endo thelium and blood-retinal barrier have indi cated that the corneal endothelium is the more sensitive paradigm for toxicity evalua tion of such materials [4,[17][18][19][20] vitreous at a substantial volume (25% of the total capacity of the vitreous cavity), offers no overt toxic effects on the blood-retinal bar rier, albeit the rabbit retinal vasculature is not the same as that of a human eye. Prior studies on retinal toxicity of perfluoro-n-octane indi cated that even large quantities of this liquid (80-90% replacement of vitreous volume) caused no change in the electroretinogram, although some histopathological changes were indicated where the liquid contacted the retina [11],…”

New substances for intraocular tamponades: perfluorocarbon liquids, hydrofluorocarbon liquids and hydrofluorocarbon-oligomers in vitreoretinal surgery

“…Ten days after removal of the oligomer from the rabbit eye, the ERG had returned to the normal value of the control eye (n=2; data not shown). Histological alterations of the lower retina were comparable to those after PFCL or HFCL application or to those that have been described in the upper retina after long-term administration of silicone oil [19,24,31]. They consisted in hypertrophy of Müller cells, structural disturbance of the outer plexiform layer, and occasional foam cells and vacuolisation on the inner retinal surface (Fig.…”

Intraocular Tolerance to Silicone Oils of Different Specific Gravities

“…The inflammatory reaction following ex perimental injection of highly fluorinated FS seen in this study was previously described on ly by Gabel et al [8], whereas other authors did not find any tissue response at all [5,6,9). Because FS may induce fibrocellular prolifer ation, Petersen et al [7] recommended appli cation of FS only for short-term tamponade and then to remove it after 4 weeks, replacing it by PDMS.…”

“…In contrast, PDMS caused nearly no droplet formation. Suprisingly, other authors, who examined highly fluorinated FS in rabbit eyes, did not note any emulsification at all [5,6,9). However, they injected FS in phakic eyes, so that pos sibly no oil droplets could migrate into the anterior chamber.…”

Perfluorocarbon Effects on Rabbit Blood-Retinal Barrier Permeability

“…The present data confirm other observa tions on the absence of toxicity of this com pound on the retina [7][8][9]11 ], as well as simi lar observations of lack of toxicity on the cor neal endothelium [4], The latter observation is of importance because comparisons of other toxic compounds on both corneal endo thelium and blood-retinal barrier have indi cated that the corneal endothelium is the more sensitive paradigm for toxicity evalua tion of such materials [4,[17][18][19][20] vitreous at a substantial volume (25% of the total capacity of the vitreous cavity), offers no overt toxic effects on the blood-retinal bar rier, albeit the rabbit retinal vasculature is not the same as that of a human eye. Prior studies on retinal toxicity of perfluoro-n-octane indi cated that even large quantities of this liquid (80-90% replacement of vitreous volume) caused no change in the electroretinogram, although some histopathological changes were indicated where the liquid contacted the retina [11],…”

New substances for intraocular tamponades: perfluorocarbon liquids, hydrofluorocarbon liquids and hydrofluorocarbon-oligomers in vitreoretinal surgery

“…Ten days after removal of the oligomer from the rabbit eye, the ERG had returned to the normal value of the control eye (n=2; data not shown). Histological alterations of the lower retina were comparable to those after PFCL or HFCL application or to those that have been described in the upper retina after long-term administration of silicone oil [19,24,31]. They consisted in hypertrophy of Müller cells, structural disturbance of the outer plexiform layer, and occasional foam cells and vacuolisation on the inner retinal surface (Fig.…”

Intraocular Tolerance to Silicone Oils of Different Specific Gravities

“…The inflammatory reaction following ex perimental injection of highly fluorinated FS seen in this study was previously described on ly by Gabel et al [8], whereas other authors did not find any tissue response at all [5,6,9). Because FS may induce fibrocellular prolifer ation, Petersen et al [7] recommended appli cation of FS only for short-term tamponade and then to remove it after 4 weeks, replacing it by PDMS.…”

“…In contrast, PDMS caused nearly no droplet formation. Suprisingly, other authors, who examined highly fluorinated FS in rabbit eyes, did not note any emulsification at all [5,6,9). However, they injected FS in phakic eyes, so that pos sibly no oil droplets could migrate into the anterior chamber.…”

Perfluorocarbon Effects on Rabbit Blood-Retinal Barrier Permeability

“…The present data confirm other observa tions on the absence of toxicity of this com pound on the retina [7][8][9]11 ], as well as simi lar observations of lack of toxicity on the cor neal endothelium [4], The latter observation is of importance because comparisons of other toxic compounds on both corneal endo thelium and blood-retinal barrier have indi cated that the corneal endothelium is the more sensitive paradigm for toxicity evalua tion of such materials [4,[17][18][19][20] vitreous at a substantial volume (25% of the total capacity of the vitreous cavity), offers no overt toxic effects on the blood-retinal bar rier, albeit the rabbit retinal vasculature is not the same as that of a human eye. Prior studies on retinal toxicity of perfluoro-n-octane indi cated that even large quantities of this liquid (80-90% replacement of vitreous volume) caused no change in the electroretinogram, although some histopathological changes were indicated where the liquid contacted the retina [11],…”

New substances for intraocular tamponades: perfluorocarbon liquids, hydrofluorocarbon liquids and hydrofluorocarbon-oligomers in vitreoretinal surgery

“…Ten days after removal of the oligomer from the rabbit eye, the ERG had returned to the normal value of the control eye (n=2; data not shown). Histological alterations of the lower retina were comparable to those after PFCL or HFCL application or to those that have been described in the upper retina after long-term administration of silicone oil [19,24,31]. They consisted in hypertrophy of Müller cells, structural disturbance of the outer plexiform layer, and occasional foam cells and vacuolisation on the inner retinal surface (Fig.…”

Intraocular Tolerance to Silicone Oils of Different Specific Gravities

“…The inflammatory reaction following ex perimental injection of highly fluorinated FS seen in this study was previously described on ly by Gabel et al [8], whereas other authors did not find any tissue response at all [5,6,9). Because FS may induce fibrocellular prolifer ation, Petersen et al [7] recommended appli cation of FS only for short-term tamponade and then to remove it after 4 weeks, replacing it by PDMS.…”

“…In contrast, PDMS caused nearly no droplet formation. Suprisingly, other authors, who examined highly fluorinated FS in rabbit eyes, did not note any emulsification at all [5,6,9). However, they injected FS in phakic eyes, so that pos sibly no oil droplets could migrate into the anterior chamber.…”