Fluorescent Biosensors of Intracellular Targets from Genetically Encoded Reporters to Modular Polypeptide Probes

Morris, May

doi:10.1007/s12013-009-9070-7

Cited by 63 publications

(47 citation statements)

References 197 publications

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“…This goal has been achieved via the marriage of GFP with FRET (12)(13)(14)(15)(16)(17)(18)(19)(20). In this review, I present theoretical and practical considerations that must be taken into account when designing a variety of FRET constructs using fluorescent proteins.…”

Section: Introductionmentioning

confidence: 99%

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

Miyawaki

2011

Annu. Rev. Biochem.

212

194

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Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the spatiotemporal regulation of various cellular processes. The different types of FRET and measurement techniques necessitate characterization of their specific features. Here I provide theoretical and practical comparisons of bimolecular and unimolecular FRET constructs, intensity-based and lifetime-based FRET measurements, FRET imaging using live- and fixed-cell samples, green fluorescent protein-based and chemical fluorophore-based FRET, and FRET efficiency and indices. The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed.

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Section: Introductionmentioning

confidence: 99%

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

Miyawaki

2011

Annu. Rev. Biochem.

212

194

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“…A fluorescent biosensor [15] measures the concentration of certain molecules within cellular compartments. Fluorescent biosensors can be used for monitoring various processes and analytes such as metabolites, ions, target localization, gene expression and physiological relevant changes within subcellular regions [16].…”

Section: Observationmentioning

confidence: 99%

On Visual Analytics and Evaluation in Cell Physiology: A Case Study

Jeanquartier

Holzinger

2013

Lecture Notes in Computer Science

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Abstract. In this paper we present a case study on a visual analytics (VA) process on the example of cell physiology. Following the model of Keim, we illustrate the steps required within an exploration and sensemaking process. Moreover, we demonstrate the applicability of this model and show several shortcomings in the analysis tools functionality and usability. The case study highlights the need for conducting evaluation and improvements in VA in the domain of biomedical science. The main issue is the absence of a complete toolset that supports all analysis tasks including the many steps of data preprocessing as well as end-user development. Another important issue is to enable collaboration by creating the possibility of evaluating and validating datasets, comparing it with data of other similar research groups.

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“…The number of available biosensors is rapidly increasing, and specific FRET biosensors are already available to measure the levels of second messengers, protein-protein interactions or the activation status of proteins (e.g. Rho) [for a review on biosensors, see Morris (Morris, 2010) and VanEngelenburg and Palmer (VanEngelenburg and Palmer, 2008)]. However, as the field of FRET biosensors is still an emerging area, only a few research groups have thus far used this powerful technique in vivo.…”

Section: Intravital Imaging Of Proteins Proteases Signaling Pathwaymentioning

confidence: 99%

Intravital microscopy: new insights into metastasis of tumors

Beerling

Ritsma

Vrisekoop

et al. 2011

Journal of Cell Science

126

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SummaryMetastasis, the process by which cells spread from the primary tumor to a distant site to form secondary tumors, is still not fully understood. Although histological techniques have provided important information, they give only a static image and thus compromise interpretation of this dynamic process. New advances in intravital microscopy (IVM), such as two-photon microscopy, imaging chambers, and multicolor and fluorescent resonance energy transfer imaging, have recently been used to visualize the behavior of single metastasizing cells at subcellular resolution over several days, yielding new and unexpected insights into this process. For example, IVM studies showed that tumor cells can switch between multiple invasion strategies in response to various densities of extracellular matrix. Moreover, other IVM studies showed that tumor cell migration and blood entry take place not only at the invasive front, but also within the tumor mass at tumor-associated vessels that lack an intact basement membrane. In this Commentary, we will give an overview of the recent advances in high-resolution IVM techniques and discuss some of the latest insights in the metastasis field obtained with IVM.

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Fluorescent Biosensors of Intracellular Targets from Genetically Encoded Reporters to Modular Polypeptide Probes

Cited by 63 publications

References 197 publications

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

On Visual Analytics and Evaluation in Cell Physiology: A Case Study

Intravital microscopy: new insights into metastasis of tumors

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