Fluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(ε-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations

Celiker, Tugba; Ghorbanizamani, Faezeh; Moulahoum, Hichem; Çelik, Emine Güler; Tok, Kerem; Zihnioğlu, Figen; Çiçek, Candan; Sertöz, Rüçhan; Arda, Bilgin; Göksel, Tuncay; Turhan, Kutsal; Tımur, Suna; Yagci, Yusuf

doi:10.1007/s00604-022-05244-2

Cited by 11 publications

(9 citation statements)

References 40 publications

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“…These devices can be used with smartphones as simple detectors and processors for POC tests. 93 For example, Celiker et al 121 used a synthetic approach to produce a graft fluorescent copolymer PPy-g-PCL conjugated to SARS-CoV-2 antibodies, yielding photoinduced step-growth polymerization of pyrene (Py) and ring-opening polymerization of ε-caprolactone (PCL). After the fluorescent graft copolymer was functionalized with antibodies recognizing SARS-CoV-2, this platform was adapted with a paper-based (dot-blot) bioassay system, which was tested using human nasopharyngeal samples.…”

Section: Other Colorimetric and Fluorescent Biosensorsmentioning

confidence: 99%

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

de Araujo,

Lukas,

Torres

et al. 2024

ACS Nano

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Many systems have been designed for the detection of SARS-CoV-2, which is the virus that causes COVID-19. SARS-CoV-2 is readily transmitted, resulting in the rapid spread of disease in human populations. Frequent testing at the point of care (POC) is a key aspect for controlling outbreaks caused by SARS-CoV-2 and other emerging pathogens, as the early identification of infected individuals can then be followed by appropriate measures of isolation or treatment, maximizing the chances of recovery and preventing infectious spread. Diagnostic tools used for high-frequency testing should be inexpensive, provide a rapid diagnostic response without sophisticated equipment, and be amenable to manufacturing on a large scale. The application of these devices should enable large-scale data collection, help control viral transmission, and prevent disease propagation. Here we review functional nanomaterial-based optical and electrochemical biosensors for accessible POC testing for COVID-19. These biosensors incorporate nanomaterials coupled with paper-based analytical devices and other inexpensive substrates, traditional lateral flow technology (antigen and antibody immunoassays), and innovative biosensing methods. We critically discuss the advantages and disadvantages of nanobiosensor-based approaches compared to widely used technologies such as PCR, ELISA, and LAMP. Moreover, we delineate the main technological, (bio)chemical, translational, and regulatory challenges associated with developing functional and reliable biosensors, which have prevented their translation into the clinic. Finally, we highlight how nanobiosensors, given their unique advantages over existing diagnostic tests, may help in future pandemics.

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Section: Other Colorimetric and Fluorescent Biosensorsmentioning

confidence: 99%

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

de Araujo,

Lukas,

Torres

et al. 2024

ACS Nano

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“…The fluorescence-based DNA nanobiosensor relies on detecting changes in fluorescence signals upon the binding of a fluorescent dye-labeled DNA probe to the target molecule [ 161 , 162 , 163 ]. The fluorescent dye labeled on the DNA probe typically remains unexcited when not bound to the target molecule.…”

Section: Dna Nanotechnology-enabled Biosensor For Virus Detectionmentioning

confidence: 99%

Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

2023

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Virus-related infectious diseases are serious threats to humans, which makes virus detection of great importance. Traditional virus-detection methods usually suffer from low sensitivity and specificity, are time-consuming, have a high cost, etc. Recently, DNA biosensors based on DNA nanotechnology have shown great potential in virus detection. DNA nanotechnology, specifically DNA tiles and DNA aptamers, has achieved atomic precision in nanostructure construction. Exploiting the programmable nature of DNA nanostructures, researchers have developed DNA nanobiosensors that outperform traditional virus-detection methods. This paper reviews the history of DNA tiles and DNA aptamers, and it briefly describes the Baltimore classification of virology. Moreover, the advance of virus detection by using DNA nanobiosensors is discussed in detail and compared with traditional virus-detection methods. Finally, challenges faced by DNA nanobiosensors in virus detection are summarized, and a perspective on the future development of DNA nanobiosensors in virus detection is also provided.

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“…Fluorescence-based optical biosensors employ the fluorescent labels to produce the optical signal which rely on detecting the fluorescence signal as a result of analyte capture on the transducer [12], [20], [21]. They are widely used in assay development owing to numerous commercially available fluorescent labels, uncomplicated labeling methods, multicolor fluorophores for multiplexed assays, fast response times with localized fluorescence signal, high temporal resolution and sufficient detection sensitivity [22].…”

Section: Fluorescence-based Optical Sensors For Virus Detectionmentioning

confidence: 99%

Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection

Seymour¹,

Kanik²,

Gür³

et al. 2023

Preprint

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Viral infections can endanger public health by causing serious illness, leading to pandemics and burdening healthcare systems. Moreover, in the situation of a global spread, disruptions occur in every aspect of life including business, education, and social life. Fast and accurate diagnosis of viral infections has huge implications for saving people’s lives, preventing spread of the diseases, and minimizing social and economic damages. In the last decades, polymerase chain reaction (PCR) based techniques have been frequently used to detect viruses in the clinic. However, in a situation where rapid virus detection is the primary measure in preventing the spread, as in the case of ongoing COVID-19 pandemic, disadvantages of PCR, such as long processing times and requirement of sophisticated laboratory instruments, have been faced. Due to the urgent need for accurate techniques for virus detection, biosensor systems involved in many applications in biological detection are being developed for rapid, real-time, and high-throughput detection of viruses. Among various sensing platforms, optical devices are of great interest due to their advantages such as high sensitivity and direct readout. In the current review, usability of sensing techniques depending on optical phenomena, such as fluorescence-based sensors, surface plasmon resonance (SPR), surface enhanced Raman scattering (SERS), optical resonators and interferometry-based platforms, is discussed for virus diagnostics applications. Then, we focus on an interferometric biosensor developed by our group, single-particle interferometric reflectance imaging sensor (SP-IRIS), which has the capability to visualize single nanoparticles, to demonstrate its application for digital virus detection.

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Fluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(ε-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations

Cited by 11 publications

References 40 publications

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection

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