Fluorescence Technique Description for Measuring Ca<sup>2+</sup> in Mice Sperm

Abstract

Recently, fluorescence technique becomes very useful. It can allow for addressing a fundamental problem of cellular mechanism besides characterizing the species inside the cell to facilitate the diagnostic and prognostic value. Manipulation with fluorescent dyes provides many possibilities for their use as tags, probes, and sensors. These types can be intrinsic or extrinsic to the cell. They can become not only silent observers, but also participants, modulators or disruptors of specific activities outline the biological functions can be successfully studied quantitatively and qualitatively with fluorescence techniques.

A high-throughput fluorimetric microarray with enhanced fluorescence and suppressed “coffee-ring” effects for the detection of calcium ions in blood

“…Still, most of the fluorescence detection methods may encounter with the big-size instruments2122, complex operations1819202122, time-consuming202122, low detection throughput, and especially inconvenient for the in-site application, which may have limited their applications at a large scale. Accordingly, developing a new assay for detecting Ca 2+ ions with high sensitivity, ideal portability, and high throughput is of considerable interest282930.…”