Fluctuation of bacteria isolated from elm tissues during different seasons and from different plant organs

Mocali, Stefano; Bertelli, Emanuela; Cello, Francescopaolo Di; Mengoni, Alessio; Sfalanga, A.; Viliani, Francesca; Caciotti, Anna; Tegli, Stefania; Surico, Giuseppe; Fani, Renato

doi:10.1016/s0923-2508(03)00031-7

Cited by 108 publications

(80 citation statements)

References 36 publications

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“…For example, the varying exudates of leaves impose a metabolic versatility on bacteria, a characteristic of the Pseudomonas genus (Mercier and Lindow, 2000;Misko and Germida, 2002). This preference for different habitats has already been shown in other plants Mocali et al, 2003).…”

Section: Discussionmentioning

confidence: 67%

Diversity of endophytic bacteria in Brazilian sugarcane

Magnani

Didonet²,

Cruz³

et al. 2010

Genet. Mol. Res.

124

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ABSTRACT. Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium was used to cultivate the endophytes; 32 isolates were selected for analysis. DNA was extracted and the 16S rRNA gene was partially sequenced and used for molecular identification. Gram staining, catalase and oxidase tests, and the API-20E system were used to characterize the isolates. The strains were divided into five groups, based on the 16S rRNA sequences. Group I comprised 14 representatives of the Enterobacteriaceae; group II was composed of Bacilli; group III contained one representative, Curtobacterium sp; group IV contained representatives of the Pseudomonadaceae family, and group V had one isolate with an uncultured bacterium. Four isolates were able to reduce acetylene to ethylene. Most of the bacteria isolated from the sugarcane stem and leaf tissues belonged to Enterobacteriaceae and Pseudomonaceae, respectively, demonstrating niche specificity. Overall, we found the endophytic bacteria in sugarcane to be 251 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (1): 250-258 (2010) Microbial diversity in sugarcane more diverse than previously reported.

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Section: Discussionmentioning

confidence: 67%

Diversity of endophytic bacteria in Brazilian sugarcane

Magnani

Didonet²,

Cruz³

et al. 2010

Genet. Mol. Res.

124

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show abstract

“…It has been demonstrated that N fixed by diazotrophic bacteria can be utilized by plants [24]. Endophytic bacteria have been found in different parts of tree tissues, such as roots [25], stems [26], shoots [27], leaves [28] and buds [29]. More recently, diazotrophic endophytes were isolated from conifers growing in nitrogen-poor soils.…”

Section: Introductionmentioning

confidence: 99%

Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia

Khan

Kandel

Ramos

et al. 2015

Forests

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Douglas-fir (Pseudotsuga menziesii) seedlings are periodically challenged by biotic and abiotic stresses. The ability of endophytes to colonize the interior of plants could confer benefits to host plants that may play an important role in plant adaptation to environmental changes. In this greenhouse study, nursery-grown Douglas-fir seedlings were inoculated with diazotrophic endophytes previously isolated from poplar and willow trees and grown for fifteen months in nutrient-poor conditions. Inoculated seedlings had significant increases in biomass (48%), root length (13%) and shoot height (16%) compared to the control seedlings. Characterization of these endophytes for symbiotic traits in addition to nitrogen fixation revealed that they can also solubilize phosphate and produce siderophores. Colonization was observed through fluorescent microscopy in seedlings inoculated with gfp-and mkate-tagged strains. Inoculation with beneficial endophytes could prove to be valuable for increasing the production of planting stocks in forest nurseries.

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“…Even though members of Sphingomonadaceae have membranes with an unusual structure, they are widely distributed in nature and have metabolic capabilities that can be exploited for bioremediation (56). Members of the Sphingomonadaceae family are commonly isolated from marine, soil, and plant environments (34,37). Based on phylogenetic, chemotaxonomic, and phenotypic observations, the Sphingomonas genus has been expanded to include three new genera, Sphingobium, Novosphingobium, and Sphingopyxis (45), although this expansion has been questioned (60).…”

mentioning

confidence: 99%

Identification of an rsh Gene from a Novosphingobium sp. Necessary for Quorum-Sensing Signal Accumulation

Gan

Buckley

Szegedi³

et al. 2009

J Bacteriol

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The stringent response is a mechanism by which bacteria adapt to environmental stresses and nutritional deficiencies through the synthesis and hydrolysis of (p)ppGpp by RelA/SpoT enzymes. Alphaproteobacteria and plants contain a single Rsh enzyme (named for RelA/SpoT homolog) that is bifunctional. Here we report the identification of a new species of bacteria belonging to the genus Novosphingobium and characterization of an rsh mutation in this plant tumor-associated isolate. Isolate Rr 2-17, from a grapevine crown gall tumor, is a member of the Novosphingobium genus that produces the N-acyl-homoserine lactone (AHL) quorum-sensing (QS) signals. A Tn5 mutant, Hx 699, deficient in AHL production was found to have an insertion in an rsh gene. The Rsh protein showed significant percent sequence identity to Rsh proteins of alphaproteobacteria. The Novosphingobium sp. rsh gene (rsh Nsp ) complemented the multiple amino acid requirements of the Escherichia coli relA spoT double mutant by restoring the growth on selection media. Besides QS signal production, the rsh mutation also affects soluble polysaccharide production and cell aggregation. Genetic complementation of the Hx 699 mutant with the rsh Nsp gene restored these phenotypes. This is the first discovery of a functional rsh gene in a member of the Novosphingobium genus.

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Fluctuation of bacteria isolated from elm tissues during different seasons and from different plant organs

Cited by 108 publications

References 36 publications

Diversity of endophytic bacteria in Brazilian sugarcane

Diversity of endophytic bacteria in Brazilian sugarcane

Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia

Identification of an rsh Gene from a Novosphingobium sp. Necessary for Quorum-Sensing Signal Accumulation

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