2014
DOI: 10.5935/1676-2444.20140014 View full text |Buy / Rent full text
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Abstract: Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementa… Show more

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“…Reticulocyte percentages were determined essentially according to the procedure described by Viana et al [ 24 ]. In brief, heparinized blood (5 µL) was mixed with 2 mL PBS and 20 µL acridine orange solution (5 µg/mL PBS, Sigma-Aldrich, Taufkirchen, Germany).…”
Section: Methodsmentioning
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“…Reticulocyte percentages were determined essentially according to the procedure described by Viana et al [ 24 ]. In brief, heparinized blood (5 µL) was mixed with 2 mL PBS and 20 µL acridine orange solution (5 µg/mL PBS, Sigma-Aldrich, Taufkirchen, Germany).…”
Section: Methodsmentioning
“…Além do laranja de tiazole, outro corante que pode ser utilizado para marcação de reticulócitos é a acridina laranja, que em protocolos padronizados para amostras de humanos demonstrou uma boa taxa de correlação quando comparada à metodologia manual (novo azul de metileno ou azul de cresil brilhante) e à metodologia automática de contadores hematológicos comerciais (VIANA et al, 2014).…”
Section: Reticulocitometriaunclassified
“…No entanto, ambos os corantes descritos possuem uma alta afinidade de ligação pelo RNA e DNA, sendo assim, as contagens reticulocitárias podem estar aumentadas quando há presença de eritrócitos nucleados nas amostras, apesar de terem um pico de excitação maior do que o referente aos reticulócitos (REAGAN et al 1992;RILEY et al 2001;VIANA et al 2014).…”
Section: Reticulocitometriaunclassified
“…In pursuit of rapid, cultivation-independent methods to quantify bacteria, fluorescence detection has proven promising. Acridine orange (AO) is one of the most commonly used fluorescence dyes for cell enumeration and has been for the last three decades [ 6 8 ]. AO is an inexpensive nucleic acid stain, fluorescing green when it binds to double-stranded DNA and red orange when it binds to single-stranded DNA or RNA.…”
Section: Introductionmentioning