‘Florin’: A Doubled Haploid Wheat Variety Developed by the Anther Culture Method

Buyser, J. De; Henry, Y.; Lonnet, Philippe; Hertzog, R.; Hespel, A.

doi:10.1111/j.1439-0523.1987.tb01089.x

Cited by 126 publications

(34 citation statements)

References 5 publications

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“…Seweral new wheat varieties bred in this way have been released in China (Hu and Zeng 1984) and France (De Buyser et al 1987).…”

Section: Anther and Pollen Culture For Haploid Formationmentioning

confidence: 99%

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Heberle‐Bors

1989

Sexual Plant Reprod

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In the past, in vitro cultures of excised anthers and isolated pollen have been used to study normal male sexual development (gametophytic development) and, conversely, to produce and study haploid plant formation (sporophytic development). For years both branches have existed side by side, without much interaction. Today, a synthesis of the two branches is possible as well as necessary. Recent advances in the technique of isolated pollen culture in the tobacco plant model (Nicotiana tabacum L.) enable the researcher to strictly control pollen development in both the gametophytic and sporophytic direction. The nutritional status of the immature pollen grain at a particular stage of development provides the trigger for its development into one of the two phases found in the alternation of generations undergone by higher plants. In particular, a hunger signal is responsible for the derepression of cell division activity and the start of embryogenesis. Pollen starvation can occur in isolated pollen cultures in sucrose-free media, in excised anthers and flowers, and, under specific growth conditions, during pollen development in vivo.

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“…Seweral new wheat varieties bred in this way have been released in China (Hu and Zeng 1984) and France (De Buyser et al 1987).…”

Section: Anther and Pollen Culture For Haploid Formationmentioning

confidence: 99%

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Heberle‐Bors

1989

Sexual Plant Reprod

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“…Homozygous doubled haploid (DH) plants are very useful for haploid breeding and genetic studies (De Buyser et al, 1987;Cadalen et al, 1998;Massiah et al, 2001). The anther culture method, in addition to wide hybridization, is a common way to produce DH-plants in cereals (Sopory and Munshi, 1996), but low regeneration rates have restricted the use of this technique in oat (Avena sativa L.).…”

Section: Introductionmentioning

confidence: 97%

Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines

Kiviharju

Moisander

Laurila

2005

Plant Cell Tiss Organ Cult

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To improve plant regeneration from oat (Avena sativa L.) anther culture, the effects of induction medium supplements and culture conditions were studied. Significantly better plant regeneration rates were obtained with cultivars Lisbeth (naked type) and Aslak when a medium containing W 14 salts and vitamins, supplemented with 2,4-D D , BAP, Ethephon, L L -cysteine and myo-inositol, was used for induction in the dark compared with a medium containing only 2,4-D D and kinetin. Genotypes reacted differently on the light during the induction phase. Use of dim light significantly decreased the green plant regeneration rates in cv. Lisbeth, while in cv. Aslak the difference was not so clear. Up to 30 green plants per 100 anthers were recovered from Aslak · Lisbeth progeny and in total, over 500 oat regenerants were produced. With these numbers, acceptable rates of DH-production for cultivar breeding and genetic study purposes are approached. The agronomic performance of some DH lines was compared with that of the plants derived from commercial seeds of the same cultivars in the field experiment. A few differences were found, but generally DH lines yielded the same or more as the commercial cultivars.

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“…l, a winter wheat variety, in only six years is testimony to the usefulness of this procedure [5]. The release of the new variety, Florin, developed via anther culture in only seven years, is further evidence of the potential value of the technique to wheat improvement [2]. The major limitation to the widespread application of anther culture to wheat breeding is related to the relatively low frequencies of anther response and plant regeneration across a broad spectrum of elite germplasm [10].…”

Section: Introductionmentioning

confidence: 99%

Effects of support medium on embryo and plant production from cultured anthers of soft-red winter wheat (Triticum aestivum L.)

Jones¹,

Petolino²

1988

Plant Cell Tiss Organ Cult

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The present study was designed to examine the effects of media support on the frequency of embryo and plant production from cultured anthers of soft-red winter wheat. Approximately twice as many embryos were produced when anthers were cultured in a liquid as compared to an agar-solidified medium. Upon transfer to regeneration medium, a significantly lower percentage of the embryos produced in liquid regenerated plants. The addition of activated charcoal to an agar-solidified medium resulted in a considerable increase in embryo production, however, plant regeneration from embryos produced on charcoalcontaining medium was significantly lower than those produced on agar only. Embryo production frequencies ranged from 2.4-13.2 and 2.5-32.2 embryos per 100 anthers on media with and without charcoal, respectively. Plant regeneration frequencies from embryos produced in the presence of activated charcoal ranged from 0-5.5% as compared to 0-39.1% from embryos produced in the absence of charcoal. More than twice as many embryos produced on Ficoll-containing liquid medium regenerated plants when compared to embryos produced in liquid only. The results from this study suggest that cultural modifications designed to maximize embryo production must take into account the quality of the resulting embryos as they relate to plant regeneration.

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‘Florin’: A Doubled Haploid Wheat Variety Developed by the Anther Culture Method

Cited by 126 publications

References 5 publications

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines

Effects of support medium on embryo and plant production from cultured anthers of soft-red winter wheat (Triticum aestivum L.)

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