Flavonoids in Selected Mediterranean Fruits: Extraction, Electrochemical Detection and Total Antioxidant Capacity Evaluation

Issaad, Fatima Zohra; Fernandes, Isabel P.G.; Enache, Teodor Adrian; Mouats, Chabane; Rodrigues, Isaide de Araujo; Oliveira-Brett, Ana Maria

doi:10.1002/elan.201600370

Cited by 16 publications

(14 citation statements)

References 38 publications

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“…Flavonoids are phenolic compounds that exhibit various biological activities, such as anti-cancer, anti-allergenic, anti-viral, anti-inflammatory effects, vasodilating actions and gastroprotective properties, as well as having superior antioxidant activities [ 30 ]. The TFC of soluble, insoluble-bound and total flavonoids extracts in CMs ranged from 0.93–3.05 μmol CE/g DS, 0.10–0.30 μmol CE/g DS and 1.23–3.14 μmol CE/g DS, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Yang

et al. 2018

Molecules

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Mistletoes are used medicinally in order to treat various human illnesses. Few studies have reported on the phenolic content and antioxidant properties of Chinese mistletoes (CMs). In this work, the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of soluble and insoluble-bound phenolic extracts from CMs hosted by Camellia assamica (Mast.) Chang (CMC) and Pyrus, i, f. (CMP) were compared. Phenolic compounds in CMC and CMP were identified and quantified using high-performance liquid chromatography (HPLC). The results indicated that the TPC of soluble phenolic extracts was higher than insoluble-bound phenolic counterparts in both CMC and CMP. In addition, the TPC of soluble, insoluble-bound and total phenolic fractions (9.91 ± 0.23, 4.59 ± 0.27 and 14.50 ± 0.35 μmol ferulic acid equivalents per gram (FAE/g) dry sample) extracted from CMP were higher than those extracted from CMC. The soluble phenolic extracts in CMP showed higher antioxidant activities than those in CMC. Eighteen phenolic compounds from soluble and insoluble-bound phenolic extracts from the CMs were identified and quantified by HPLC. This study indicates that CMC and CMP, especially the latter, could be sources of antioxidants in human health care.

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Section: Resultsmentioning

confidence: 99%

“…Flavonoids, namely catechin hydrate, epicatechin, (−)-epigallocatechin, myricetin, quercetin, kaempferol and apigenin were determined. Flavonoids are a large family of compounds in plants [ 30 ]. The content of all six phenolic compounds in CMP was higher than those in CMC.…”

Section: Resultsmentioning

confidence: 99%

Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Yang

et al. 2018

Molecules

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“…The HPLC‐EC conditions have already been optimized and enabled the electrochemical detection and identification of the flavonoid standards at a much lower concentration than the PDA, and without interferences. The retention times ( t r ) obtained for all control flavonoid standards are in Table .…”

Section: Resultsmentioning

confidence: 99%

“…The HPLC‐ED separation optimization process, and the elution gradient used, is described in . A programmed sequence of gradient elution from the mobile phase A to the mobile phase B HPLC experimental conditions was: isocratic elution 100 % mobile phase A, for first 11 minutes, followed by linear gradient elution from 100 % mobile phase A to 100 % mobile phase B, between 11 and 21 minutes, and an isocratic elution 100 % mobile phase B, from 21 to 60 minutes , to accomplish the complete elution of each wine sample.…”

Section: Methodsmentioning

confidence: 99%

Phenolic Composition and Total Antioxidant Capacity by Electrochemical, Spectrophotometric and HPLC‐EC Evaluation in Portuguese Red and White Wines

et al. 2019

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Phenolic composition and in vitro total antioxidant capacity have been evaluated, in red and white Portuguese wines, by electrochemical methods, spectrophotometric methods, and reverse‐phase high performance liquid chromatography with electrochemical detection (HPLC‐EC). The different phenolic compounds present in seventeen red and white wines, with different grape varieties, and from different geographical locations, were successfully identified and detected. The wines in vitro total antioxidant capacity, using the method of capture of diphenilpicrilhydrazil (DPPH.) free radical “efficient concentration” (EC50), and the electrochemical quantitative index (EI), was determined. The wine with the highest total antioxidant capacity exhibited the lowest antioxidant power, and the EI and EC50 assays had a very good correlation.

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“…Thus, GSH can be utilized for the characterization of the oxidation products of phenolic compounds. Furthermore, it is known that electrochemical techniques provide valuable insights into the underlying reaction mechanisms of flavonoids as well as their relevant antioxidant activity . Evidently, the oxidation products resulting from the oxidative reactions of Er are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Pathways and Identification of the Electrochemically Generated Oxidation Products of Flavonoid Eriodictyol in the Presence of Glutathione

et al. 2018

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The metabolic oxidation pathways of dietary flavonoid eriodictyol (Er) are not very well‐probed. In the present work, the electrochemical oxidation behavior of Er was studied in aqueous Britton‐Robinson (B‐R) buffer solution using cyclic voltammetry (CV), chronoamperometry (CA), and bulk‐electrolysis (BE). The oxidation products and reaction pathways of Er in the absence and the presence of glutathione (GSH) were proposed and identified in view of the results obtained by ultra‐high‐performance liquid chromatography coupled with mass spectrometry (UPLC‐MS). In the absence of GSH, eriodictyol shows one quasi‐reversible oxidation process at E1/2=0.305 V, followed by a totally irreversible anodic peak at a more positive potential (Epa=1.05 V vs. Ag/AgCl, 3 M KCl). Putatively, the first process corresponds to the oxidation of the catechol moiety on the B ring of Er while the second one is attributed to the oxidation of the resorcinol moiety on the A ring. In the presence of GSH, however, the anodic oxidation of Er was proposed to be an ECEC‐type mechanism. The Er molecule first underwent a two‐electron oxidation coupled with loss of two‐proton to generate the corresponding quinone, which was either reduced to the original Er molecule by GSH, or further interacted with GSH to produce mono‐ and bi‐ glutathione conjugates of Er. The proposed mechanism was confirmed by digital simulation of the cyclic voltammograms.

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Flavonoids in Selected Mediterranean Fruits: Extraction, Electrochemical Detection and Total Antioxidant Capacity Evaluation

Cited by 16 publications

References 38 publications

Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Phenolic Composition and Total Antioxidant Capacity by Electrochemical, Spectrophotometric and HPLC‐EC Evaluation in Portuguese Red and White Wines

Mechanistic Pathways and Identification of the Electrochemically Generated Oxidation Products of Flavonoid Eriodictyol in the Presence of Glutathione

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