Flavonoids from Ulex airensis and Ulex europaeus ssp. europaeus

Máximo, Patrícia; Lourenço, Ana; Feio, Sonia Savluchinske; Roseiro, J. Carlos

doi:10.1021/np010147j

Cited by 35 publications

(21 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Isowighteone has been proven to be both antibacterial (Hatano et al, 2000) and antifungal (Ingham, 1990;Máximo et al, 2002;Queiroz et al, 2002;Hostettmann et al, 2005). However, it is expensive to extract directly from plant materials because of its low level.…”

Section: Lapt1 As a Tool For Metabolic Engineeringmentioning

confidence: 99%

Characterization of an Isoflavonoid-Specific Prenyltransferase from Lupinus albus

et al. 2012

View full text Add to dashboard Cite

Prenylated flavonoids and isoflavonoids possess antimicrobial activity against fungal pathogens of plants. However, only a few plant flavonoid and isoflavonoid prenyltransferase genes have been identified to date. In this study, an isoflavonoid prenyltransferase gene, designated as LaPT1, was identified from white lupin (Lupinus albus). The deduced protein sequence of LaPT1 shared high homologies with known flavonoid and isoflavonoid prenyltransferases. The LaPT1 gene was mainly expressed in roots, a major site for constitutive accumulation of prenylated isoflavones in white lupin. LaPT1 is predicted to be a membrane-bound protein with nine transmembrane regions and conserved functional domains similar to other flavonoid and isoflavonoid prenyltransferases; it has a predicted chloroplast transit peptide and is plastid localized. A microsomal fraction containing recombinant LaPT1 prenylated the isoflavone genistein at the B-ring 39 position to produce isowighteone. The enzyme is also active with 29-hydroxygenistein but has no activity with other flavonoid substrates. The apparent K m of recombinant LaPT1 for the dimethylallyl diphosphate prenyl donor is in a similar range to that of other flavonoid prenyltransferases, but the apparent catalytic efficiency with genistein is considerably higher. Removal of the transit peptide increased the apparent overall activity but also increased the K m . Medicago truncatula hairy roots expressing LaPT1 accumulated isowighteone, a compound that is not naturally produced in this species, indicating a strategy for metabolic engineering of novel antimicrobial compounds in legumes.

show abstract

Section: Lapt1 As a Tool For Metabolic Engineeringmentioning

confidence: 99%

Characterization of an Isoflavonoid-Specific Prenyltransferase from Lupinus albus

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The known prenylated isoflavones derrone (2), 19 alpinum isoflavone (3), 19,20 and wighteone (4), 18 were identified by comparing their NMR data with those reported in the literature. Derrone (2) and alpinum isoflavone (3) are isomers, possessing isoprenyl groups linked at C-8 and C-6, respectively.…”

Section: Resultsmentioning

confidence: 99%

Prenylated flavonoids from maclura tinctoria fruits

et al. 2013

View full text Add to dashboard Cite

Federal do Acre, 69915-900 Rio Branco-AC, BrasilRecebido em 13/8/12; aceito em 4/2/13; publicado na web em 24/5/13 A phytochemical investigation of Maclura tinctoria fruits yielded five flavonoids, including one prenylated flavonol (licoflavonol) and four prenylated isoflavones (wighteone, derrone, alpinum isoflavone, and 6-(2,2-dimethyl-2H-chromen-6-yl)-2-(1-hydroxy-1-methylethyl)-2,3-dihydro-5H-furo[3,2-g]chromen-5-one). The structures of the isolates were established by analyzing their spectroscopic data. Compound 6-(2,2-dimethyl-2H-chromen-6-yl)-2-(1-hydroxy-1-methylethyl)-2,3-dihydro-5H-furo[3,2-g]chromen-5-one is new and the other isolates are described for the first time in this species.

show abstract

“…Notably, the high content of extractives (6.6%) was larger than in normal industrial biomass resources. This could be attributed to the high content of tannins, flavonoids, and isoprenoids (Cao et al 1997;Máximo et al 2002) in Ulex. This was part of a larger process aimed at using supercritical fluids for the pre-processing of this otherwise unused biomass.…”

Section: Stem Compositionmentioning

confidence: 99%

“…The fibres were therefore made of small Iβ domains embedded in an amorphous matrix (Atalla and Vanderhart 1984). This microstructure had a direct influence on the intrinsic properties (Gardner and Blackwell 1974;Atalla and Vanderhart 1984;Sugiyama et al 1991;Wada et al 1993;Cao et al 1997;Máximo et al 2002;Nishiyama et al 2002;Dimmel and Gellerstedt 2009;Leppänen et al 2009;Poletto et al 2014). The change in crystallinity was probably due to the growth of those domains accelerated by the heat involved in the fibreboard consolidation (Salmén 2004).…”

Section: Structure/microstructure and Thermal Analysis Of Cellulosementioning

confidence: 99%

Exploring Ulex europaeus to Produce Nontoxic Binderless Fibreboard

Pesenti¹,

Torres²,

Oliveira³

et al. 2017

BioResources

View full text Add to dashboard Cite

Ulex europaeus is one of the most abundant and aggressively invasive plants on the world. Its fibres, which can be isolated using an alkaline pulping process, have been successfully thermo-pressed into high-density fibreboards without any type of binder. The influence of the bioorganic and crystalline components on the product was investigated using crystallographic, thermo-analytical, and mechanical techniques. Cellulose was predominantly an Iβ polymorph, more common in hardwood, but the composition of the material was typical of softwood. Both the crystallinity in the fibres and the average domain size of cellulose increased during thermo-pressing. Notwithstanding that the residual lignin was present in a small amount, this promoted the cohesion of fibres by improving hydrolysis and adhesion properties. The best overall properties were observed in the pressed products of 1030 ± 38 kg/m 3 , showing an elastic modulus of 4.31 ± 0.26 GPa, with a modulus of rupture of 26.5 ± 1.3 MPa. These results serve as the basis to transform the invasive species into a fully non-toxic added-value resource.

show abstract

Flavonoids from Ulex airensis and Ulex europaeus ssp. europaeus

Cited by 35 publications

References 34 publications

Characterization of an Isoflavonoid-Specific Prenyltransferase from Lupinus albus

Characterization of an Isoflavonoid-Specific Prenyltransferase from Lupinus albus

Prenylated flavonoids from maclura tinctoria fruits

Exploring Ulex europaeus to Produce Nontoxic Binderless Fibreboard

Contact Info

Product

Resources

About