Flavonoid metabolites in urine after oral administration of aqueous extract of Persea americana to rats

Abstract

In the present study, urinary excretion of the flavonoid metabolites of Persea americana was investigated. Aqueous extract of the leaves of P. americana was administered by gastric probe to Sprague-Dawley rats and urine samples were collected daily for five days. The urine samples were analyzed by reversed-phase HPLC-UV detection. The result obtained showed extensive degradation of the flavonoid of P. americana within the first day. The phenylalkyl acids detected, 3,4-dihydroxyphenylacetic acid (I), hippuric acid (II), 3-hydroxyphenylacetic acid (III), 3-methoxy-4-hydroxyphenylacetic acid (IV), 3-(4-hydroxyphenyl)propionic acid (V) and benzoic acid (VI) were confirmed by their HPLC retention times or by cochromatography with reference standards. No flavonoid glycosides or aglycones were detected in the urine samples.

Quantification of phytochemical constituents in vitro antioxidant activity of Synadium grantii

“…The biological activities of plants may be due to the presence of a diverse group of chemical compounds including steroids, glycosides, phenolics, glycosides, anthocyanins, flavonoids etc. [1][2][3] The search of biologically active compounds from plants has always been of great interest to scientists looking for new sources of useful drugs against infectious diseases. Many plant species have been investigated in the search for natural antioxidants but generally there is still a demand to find more information concerning the antioxidant potential of plant species.…”

Analysis of isoflavones and flavonoids in human urine by UHPLC

“…HPLC, with various types of detectors, for example ultra-violet (UV) [ 5 – 9 ], diode array [ 10 ], coulometric array [ 11 ], and mass spectrometry [ 12 – 14 ]), is the most common method used for determination of flavonoids in biological fluids. Flavonoids have been quantified in the presence of other antioxidants using electrochemical detection by Bolarinwa and Linseisen [ 15 ].…”

Hepato-Preventive Effects of Hydroethanolic Leaves Extract of <i>Persea americana</i> Mill. (Lauraceae) “Avocado” against Antouka Super<sup>®</sup>Induced Damage in Male Japanese Quail (<i>Coturnix coturnix</i> Japonica)

“…So, the dietary intake of natural products is considered very important for preventing a wide variety of diseases such as allergies, cardiovascular disease, certain forms of cancer, hepatic diseases, and inflammation, which involve free radical-mediated damage in pathologically generating processes [29]. Therefore, that is an essential research about suitable herbal drugs that could replace the chemical ones Owolobi et al [30]. However, the widespread use of Persea Americana in traditional medicine stimulated us to explore its potential biological activity.…”