Flash Chromatography: A Novel Pressurization Apparatus

Butler, Jeffrey D.; Choung, Wonken; Kurth, Mark J.

doi:10.1021/ed100416x

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…Flash liquid column chromatography was performed to obtain isolated compounds from stem fractions following previously described procedures [53][54][55]. A glass column filled with silica gel 60 (0.040-0.063 nm) was used for column chromatography (Ref.…”

Section: Isolation and Chemical Characterization Proceduresmentioning

confidence: 99%

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

et al. 2022

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Glioblastoma (GB) is the most malignant form of primary astrocytoma, accounting for more than 60% of all brain tumors in adults. Nowadays, due to the development of multidrug resistance causing relapses to the current treatments and the development of severe side effects resulting in reduced survival rates, new therapeutic approaches are needed. The genus Plectranthus belongs to the Lamiaceae family and is known to be rich in abietane-type diterpenes, which possess antitumor activity. Specifically, P. hadiensis (Forssk.) Schweinf. ex Sprenger has been documented for the use against brain tumors. Therefore, the aim of this work was to perform the bioguided isolation of compounds from the acetonic extract of P. hadiensis stems and to investigate the in vitro antiglioblastoma activity of the extract and its isolated constituents. After extraction, six fractions were obtained from the acetonic extract of P. hadiensis stems. In a preliminary biological screening, the fractions V and III showed the highest antioxidant and antimicrobial activities. None of the fractions were toxic in the Artemia salina assay. We obtained different abietane-type diterpenes such as 7α-acetoxy-6β-hydroxyroyleanone (Roy) and 6β,7β-dihydroxyroyleanone (DiRoy), which was also in agreement with the HPLC-DAD profile of the extract. Furthermore, the antiproliferative activity was assessed in a glioma tumor cell line panel by the Alamar blue assay. After 48 h treatment, Roy exerted strong antiproliferative/cytotoxic effects against tumor cells with low IC50 values among the different cell lines. Finally, we synthesized a new fluorescence derivative in this study to evaluate the biodistribution of Roy. The uptake of BODIPY-7α-acetoxy-6β-hydroxyroyleanone by GB cells was associated with increased intracellular fluorescence, supporting the antiproliferative effects of Roy. In conclusion, Roy is a promising natural compound that may serve as a lead compound for further derivatization to develop future therapeutic strategies against GB.

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Section: Isolation and Chemical Characterization Proceduresmentioning

confidence: 99%

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

et al. 2022

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“…A further advantage of this system is that additional eluting solvent can be added directly through the open branch of the Y. If house nitrogen is not available, compressed air should also work, and other means of creating pressure for flash chromatography may be adapted. − Alternatively, attaching a piece of tubing to the stopcock at the bottom of the column and using a large syringe to pull solvents though the column is also successful. Any of these methods significantly decrease elution time compared to a gravity flow column.…”

Section: Experimental Considerationsmentioning

confidence: 99%

Using Ion Exchange Chromatography To Separate and Quantify Complex Ions

Johnson

2014

J. Chem. Educ.

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Ion exchange chromatography is an important technique in the separation of charged species, particularly in biological, inorganic, and environmental samples. In this experiment, students are supplied with a mixture of two substitution-inert complex ions. They separate the complexes by ion exchange chromatography using a "flash" technique. After taking a visible spectrum of the eluted complexes, the unknowns are identified by comparison with a table of supplied λ max values and quantified using Beer's law. This experiment can be completed in under 4 h and is suitable for introductory and intermediate chemistry students.

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“…While this technique is commonly used by organic chemists, it has not undergone any major evolution over several decades and remains the method of choice for the purification of organic compounds. Some minor modifications were proposed to facilitate access to this technique in teaching laboratories. − However, the emergence of automated flash chromatography systems like Reveleris (Buchi), Isolera (Biotage), or Puriflash (Interchim) has deeply changed the ways compounds are purified and opened new teaching opportunities in the organic synthesis as well as in analytical chemistry. It is obvious that such devices will find their usefulness in the organic chemistry laboratory as a complement/replacement of traditional column chromatography since it became increasingly popular in companies as well as in academic research laboratories.…”

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confidence: 99%

Flash Chromatography System: A Practical Tool for Demonstrating the Influence of Column Characteristics on Chromatographic Resolution

2020

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A flash purification system using prepacked cartridges containing different silica weight and particle sizes was employed to demonstrate the influence of column characteristics on chromatographic resolution. This chromatographic experiment was a helpful introduction to chromatographic courses where the influence of particle sizes was addressed only theoretically and it allows students to experience the concept in an active learning style experiment. After running a predefined set of experiments to understand concepts, students were asked to design their own experiment to achieve the best chromatographic resolution. Moreover, the difference of mass versus concentration detectors was highlighted using Ultraviolet (UV) and Evaporative Light Scattering Detectors (ELSD). Questionnaires were also used to define the students' initial knowledge and to assess on what they learned following this lab.

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Flash Chromatography: A Novel Pressurization Apparatus

Cited by 6 publications

References 10 publications

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

Using Ion Exchange Chromatography To Separate and Quantify Complex Ions

Flash Chromatography System: A Practical Tool for Demonstrating the Influence of Column Characteristics on Chromatographic Resolution

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