Five-Minute Treatments With Fluorouracil, Floxuridine, and Mitomycin Have Long-term Effects on Human Tenon's Capsule Fibroblasts

Validation of the Glaucoma Filtration Surgical Mouse Model for Antifibrotic Drug Evaluation

“…The profound cytotoxic effect of MMC in the proliferation phases of Tenon's fibroblasts (35)(36)(37) was demonstrated clearly in our model where the MMCtreated conjunctiva contained no detectable proliferative cells compared with the control wound site, which did contain proliferative cells. Mouse conjunctival fibroblasts cultured in vitro showed the same antiproliferative response to MMC.…”

Trabeculectomy with or without mitomycin-C for paediatric glaucoma in aphakia and pseudophakia following congenital cataract surgery

“…Several prospective randomized studies in adults with high-risk glaucoma filtering surgery have shown that intraoperative MMC may be a better alternative to the use of postoperative 5-FU because MMC-augmented trabeculectomy is shown to have resulted in lower overall IOP, decreased dependence on postoperative antiglaucoma medication, and decreased corneal toxicity. [31][32][33] Khaw et al, 34 in an elegant study of cell culture analysis, demonstrated the greater effect of MMC as compared to 5-FU on both fibroblast proliferation and morphologic characteristics. Encouraging reports of intraoperative use of MMC in adults, [31][32][33] coupled with the difficulties encountered by repeated injections of 5-FU in children, prompted us to use MMC intraoperatively during glaucoma filtering surgery in these patients.…”

Is the single use of intraoperative 5-fluorouracil in filtering surgery for high-risk cases enough?

“…Khaw et al 20 (Table 4), the conclusion one could draw from them and our study is that the single use of 5FU intraoperatively may prolong the survival of filtering procedures in patients with either no risk factors or low to moderate risk factors.…”