First Systematic CGH-based Analyses of Ancient DNA Samples of Malformed Fetuses Preserved in the Meckel Anatomical Collection in Halle/Saale (Germany)

Tönnies, Holger; Gerlach, Antje; Klunker, Rudyard; Schultka, Rüdiger; Göbbel, Luminita

doi:10.1369/jhc.4b6427.2005

Cited by 6 publications

(4 citation statements)

References 8 publications

(12 reference statements)

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“…A research project was started, in which molecular cytogenetic techniques are used in combination with radiographical techniques, computer tomography (CT), spiral CT, and magnetic resonance imaging (MRI) to diagnose such anomalies. Umbilical cord biopsies were taken to test for chromosome imbalances, which could have caused the malformations [Tönnies et al, 2002, 2005]. The re‐examination and diagnosis of the human fetuses provides a unique opportunity to present important specimens from the Meckel Collection investigated with modern techniques.…”

Section: Introductionmentioning

confidence: 99%

Improvement of bonding strength between biomimetic apatite coating and substrate

Wei

2007

J Biomed Mater Res

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Bone-like apatite coatings were prepared using a biomimetic method in a modified simulated body fluid (m-SBF). The effect of the m-SBF volume on the apatite coating quality was studied. Three m-SBF volumes, 50, 100, and 200 mL, were employed to immerse titanium substrates in a sealed container so as to produce apatite coatings with different properties, namely types I, type II, and type III apatite coatings, respectively. The coatings were characterized using X-ray diffraction and environmental scanning electron microscope. The bonding between the coating and the Ti substrate was evaluated using an adhesive strength test. All three apatite coatings demonstrated a poorly crystallized structure, and the coatings formed exhibited a uniformed surface morphology. Further increasing the m-SBF volume, small globules of apatite started to form on the surface of the coating. The bonding strength for the three coating systems were 8.52 +/- 2.41, 10.36 +/- 2.78, and 17.23 +/- 2.55 MPa for types I, II, and III apatite coatings, respectively. The failure analyses suggested that type III coating failed mostly at the interface between the coating and the substrate, while type I and II coatings failed mostly within the apatite coating. Our study revealed that a dense, thick, well-adhered apatite coating could be achieved by carefully controlling the volume of m-SBF.

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Section: Introductionmentioning

confidence: 99%

Improvement of bonding strength between biomimetic apatite coating and substrate

Wei

2007

J Biomed Mater Res

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“…It could also serve as a useful tool in diagnostic definition of museum specimens and, more generally, in teaching anatomo-radiological correlations [17]. Furthermore, the application of modern immunohistochemical and molecular techniques to historical long-term preserved fixed tissues has proven feasible, opening new lines of research [18,19].…”

Section: Strategies and Challenges For Contemporary Pathology Museumsmentioning

confidence: 99%

Voices from the past: results of the ESP history of pathology working group survey on pathology museums

et al. 2022

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While keeping their original purpose of training medical students, pathology museums hold great biological value, offering unique specimens for scientific research through modern radiological, pathological and biomolecular techniques. Moreover, the artefacts, models and drawings displayed in these museums are a precious cultural and artistic heritage. Preservation of the anatomical samples and maintenance of the facilities are neither easy nor inexpensive and call for patronage. The development of a European Pathology Museum Network would undoubtedly facilitate study, access and divulgation of antique pathology collections. Data from a survey conducted by the European Society of Pathology (ESP) History of Pathology Working Group have allowed creation of a comprehensive, multifaceted portrait of European university museums, reflecting their history, diversity, geography, institutional status, stakeholders, projects, professionals, audiences, policies and best practices.

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“…In the last few years, the collection, and in particular the anomalous human and animal fetuses were studied to identify the specimens described by Meckel the Younger and his students [Klunker, 2003]. DNA‐based molecular cytogenetic techniques, such as comparative genomic hybridization (CGH), were used in combination with radiographic techniques, computer tomography (CT), spiral CT, and magnetic resonance imaging (MRI) to reassess the diagnoses from a contemporary perspective and to compare these with the original diagnoses [Klunker et al, 2002, 2005; Tönnies et al, 2002, 2005; Göbbel et al, 2005]. The collection includes many malformations which Meckel the Younger called either anomalies of incomplete development or primary malformations ( die ursprünglichen Bildungsfehler ).…”

Section: Introductionmentioning

confidence: 99%

Nuchal cystic hygroma in five fetuses from 1819 to 1826 in the Meckel‐anatomical collections at the University of Halle, Germany

Göbbel¹,

Schultka²,

Klunker³

et al. 2006

American J of Med Genetics Pt A

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The Anatomical collection of the Department of Anatomy and Cell Biology, Medical School of the University of Halle, Germany, comprises more than 8,000 specimens. Around 600 of them show congenital anomalies. The collection of abnormal human and animal fetuses began as the private collection of Johann Friedrich Meckel the Elder (1724-1774), his son Philipp Friedrich Theodor Meckel (1755-1803) and his grandson Johann Friedrich Meckel the Younger (1781-1833). Meckel the Younger founded the systematic science of developmental pathology. Radiographical techniques, computer tomographic (CT) methods, magnetic resonance imaging (MRI), and comparative genomic hybridization (CGH) were used to diagnose abnormal human fetuses in the Meckel-anatomical collections. Cystic hygroma colli was found in five of the human fetuses originally described by JF Meckel the Younger in 1826 and one of his students in 1819 [Hencke, 1819]. CGH analyses were used to test whether the observed cystic hygroma colli could be caused by chromosomal aneuploidies. CGH-ratio profiles of all chromosomes were apparently normal. PCR-based sex determination tests on ancient DNA were used to determine the fetal gonosomal constitution. It is likely that the Meckel specimens are among the oldest fetuses in which Ullrich-Turner "phenotype" has been diagnosed.

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First Systematic CGH-based Analyses of Ancient DNA Samples of Malformed Fetuses Preserved in the Meckel Anatomical Collection in Halle/Saale (Germany)

Cited by 6 publications

References 8 publications

Improvement of bonding strength between biomimetic apatite coating and substrate

Improvement of bonding strength between biomimetic apatite coating and substrate

Voices from the past: results of the ESP history of pathology working group survey on pathology museums

Nuchal cystic hygroma in five fetuses from 1819 to 1826 in the Meckel‐anatomical collections at the University of Halle, Germany

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