First report of Wohlfahrtiimonas chitiniclastica bacteraemia in South Africa

Hoffman, R.J.; Fortuin, Franklin; Newton-Foot, Mae; Singh, Sandhya

doi:10.7196/samj.2016.v106i11.11449

Cited by 8 publications

(8 citation statements)

References 4 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, four isolates harbor sulfonamide resistance genes, while the remaining 22 strains appear to be susceptible. This is in line with recent case reports, in which W. chitiniclastica was susceptible to diverse antibiotics including trimethoprim/sulfamethoxazole ( Chavez et al., 2017 ; Katanami et al., 2018 ; Connelly et al., 2019 ; Snyder et al., 2020 ; Bueide et al., 2021 ), while the first reported case in South Africa surprised with trimethoprim/sulfamethoxazole resistance ( Hoffmann et al., 2016 ). A similar picture was observed for tetracycline resistance, which showed a rather diverse distribution among the isolates.…”

Section: Discussionsupporting

confidence: 90%

Comparative Genomic Analysis of the Human Pathogen Wohlfahrtiimonas Chitiniclastica Provides Insight Into the Identification of Antimicrobial Resistance Genotypes and Potential Virulence Traits

Kopf¹,

Bunk²,

Coldewey³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Recent studies suggest that Wohlfahrtiimonas chitiniclastica may be the cause of several diseases in humans including sepsis and bacteremia making the bacterium as a previously underappreciated human pathogen. However, very little is known about the pathogenicity and genetic potential of W. chitiniclastica; therefore, it is necessary to conduct systematic studies to gain a deeper understanding of its virulence characteristics and treatment options. In this study, the entire genetic repertoire of all publicly available W. chitiniclastica genomes was examined including in silico characterization of bacteriophage content, antibiotic resistome, and putative virulence profile. The pan-genome of W. chitiniclastica comprises 3819 genes with 1622 core genes (43%) indicating a putative metabolic conserved species. Furthermore, in silico analysis indicated presumed resistome expansion as defined by the presence of genome-encoded transposons and bacteriophages. While macrolide resistance genes macA and macB are located within the core genome, additional antimicrobial resistance genotypes for tetracycline (tetH, tetB, and tetD), aminoglycosides (ant(2’’)-Ia, aac(6’)-Ia,aph(3’’)-Ib, aph(3’)-Ia, and aph(6)-Id)), sulfonamide (sul2), streptomycin (strA), chloramphenicol (cat3), and beta-lactamase (blaVEB) are distributed among the accessory genome. Notably, our data indicate that the type strain DSM 18708T does not encode any additional clinically relevant antibiotic resistance genes, whereas drug resistance is increasing within the W. chitiniclastica clade. This trend should be monitored with caution. To the best of our knowledge, this is the first comprehensive genome analysis of this species, providing new insights into the genome of this opportunistic human pathogen.

show abstract

Section: Discussionsupporting

confidence: 90%

Comparative Genomic Analysis of the Human Pathogen Wohlfahrtiimonas Chitiniclastica Provides Insight Into the Identification of Antimicrobial Resistance Genotypes and Potential Virulence Traits

Kopf¹,

Bunk²,

Coldewey³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Nevertheless, antimicrobial resistome analysis prior to treatment is always recommended as newly emerged resistance might arise quickly. For example, the first reported case of W. chitiniclastica infection in South Africa surprised with trimethoprim/sulfamethoxazole resistance ( Hoffmann et al, 2016 ). To the best of our knowledge, this is the first report in which an isolate appears to be resistant.…”

Section: Discussionmentioning

confidence: 99%

Identification and Antibiotic Profiling of Wohlfahrtiimonas chitiniclastica, an Underestimated Human Pathogen

et al. 2021

View full text Add to dashboard Cite

In the past 12 years, several case reports have clearly demonstrated that Wohlfahrtiimonas chitiniclastica is capable of causing sepsis and bacteremia in humans. However, since most clinicians are not familiar with this species, little is known about its pathogenicity and treatment options while it is as rare but underestimated human pathogen. Therefore, a larger strain collection is required so that methods can be identified that are most suitable to obtain rapid and reliable identification. Moreover, the antimicrobial resistance profile needs to be elucidated in order to explore possible treatment options. Over a period of 6 years, we therefore have collected a total of 14 W. chitiniclastica isolates in routine diagnostics, which now served as the basis for a comprehensive characterization with respect to identification and antibiotic profiling. We compared the accuracy and convenience of several identification techniques in which MALDI-TOF MS and sequencing of the 16S rRNA gene have proven to be suitable for identification of W. chitiniclastica. In addition, whole genome sequencing (WGS)-based digital DNA-DNA hybridization (dDDH) was used as a reference method for strain identification, and surprised with the detection of a novel W. chitiniclastica subspecies. A combination of in silico and in vitro analyses revealed a first insight into the antimicrobial resistance profile and the molecular basis of antimicrobial resistance. Based on our findings, trimethoprim/sulfamethoxazole, levofloxacin, and cephalosporins (e.g., ceftazidime) may be the best antibiotics to use in order to treat infections caused by W. chitiniclastica, while resistance to fosfomycin, amikacin and tobramycin is observed.

show abstract

“…Previous human cases of W. chitiniclastica have occurred globally including France, Argentina, Estonia, the United Kingdom, Japan, Australia, India, South Africa, Spain, and Malaysia, Hawaii, Germany, and the continental United States [7][8][9][10][11][12][13][14][15][16][17]. Infection is associated with social and medical circumstances that lead to poor sanitation, underlying medical conditions compromising normal host immune function, and chronic open wounds.…”

Section: Discussionmentioning

confidence: 99%

“…Of these, five had a single pathogen in the blood. Three were primarily treated with a cephalosporin [7,15,17] while two cases including this one had a quinolone in the regimen. There were nine patient reports that had W. chitiniclastica as part of a polymicrobial blood infection and most utilized a beta-lactam antibiotic [5,6,9,[11][12][13][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Maggot Wound Therapy Associated with Wohlfahrtiimonas chitiniclastica Blood Infection

Bueide¹,

Hunt²,

Bande³

et al. 2021

Cureus

View full text Add to dashboard Cite

Maggot therapy is the intentional application of live, disinfected fly larvae to wounds for debridement and wound care. Although some studies suggest possible beneficial applications for wound healing, subsequent infection is a potential risk of treatment. We present a case of a 70-year-old male with chronic left temporal wound from squamous cell carcinoma treated with maggot therapy complicated by bacteremia with Wohlfahrtiimonas chitiniclastica (W. chitiniclastica) . This case illustrates the risk for W. chitiniclastica infection associated with maggots used in medical wound therapy of chronic wounds.

show abstract

First report of Wohlfahrtiimonas chitiniclastica bacteraemia in South Africa

Cited by 8 publications

References 4 publications

Comparative Genomic Analysis of the Human Pathogen Wohlfahrtiimonas Chitiniclastica Provides Insight Into the Identification of Antimicrobial Resistance Genotypes and Potential Virulence Traits

Comparative Genomic Analysis of the Human Pathogen Wohlfahrtiimonas Chitiniclastica Provides Insight Into the Identification of Antimicrobial Resistance Genotypes and Potential Virulence Traits

Identification and Antibiotic Profiling of Wohlfahrtiimonas chitiniclastica, an Underestimated Human Pathogen

Maggot Wound Therapy Associated with Wohlfahrtiimonas chitiniclastica Blood Infection

Contact Info

Product

Resources

About