First Report of Saprolegnia parasitica from a Marine Species: Gilthead Seabream (Sparus aurata) in Brackish Water Conditions

Abstract

Saprolegniosis is a serious fungal disease that mostly affects freshwater fish species and eggs. It has a cotton wool-like appearance on the body of amphibians, crustaceans and several fish species. Infected gilthead seabream (Sparus aurata) were subjected to clinical, microbiological, parasitological and pathological investigation. On the infected skin samples, grey-white cotton-like patches, erosion of the skin and scale affusion were detected. Lesions covered the whole body of S. aurata in the advanced stages. Bacterial growth and parasitic symptoms were not observed in microbiological examination. Microscopic examination showed hyphaes carrying cysts that were long and branched. In scanning electron microscopy overviews fungal zoospores were observed. In histopathological observations of sections of skin, erosive-ulcerative dermatitis and mycelium of Saprolegnia parasitica were seen in the muscle tissue. Gene sequence-based identification found Saprolegnia parasitica. S. parasitica has not until now been detected in S.aurata. The low salinity of the brackish water is believed to be the predisposing factor of Saprolegniosis in sea bream in this case.

Cox2 community barcoding at Prince Edward Island reveals long-distance dispersal of a downy mildew species and potentially marine members of the Saprolegniaceae

“…The scale bar indicates the number of substitutions per site Two of the six phylotypes (represented by one OTU, each) were located within the Saprolegniaceae, with unclear genus affinity. This is noteworthy, as Saprolegnia is considered a terrestrial genus, with infrequent incursions in brackish environments (Höhnk 1968;Dinçtürk et al 2019). While it seems possible that the OTUs represent marine members of the Saprolegniaceae not observed so far, it is also conceivable that, similar to the situation in G. rostratum s.l., the saprolegniaceous species were merely washed to the sea and have a terrestrial origin.…”

Efecto Inhibitorio de Extractos Hidroalcoholicos de Larrea Tridentata Sobre Saprolegnia Sp

“…Para la identificación preliminar de las colonias, se aplicó la tinción con azul de lactofenol, con especial énfasis en las colonias blancas, algodonosas, esponjosas, ligeramente abultadas y blandas (Deutsch et al, 2023;Zaror et al, 2004). La observación se realizo bajo un microscopio con un aumento de 10x y 400x observándose la estructura y la replicación del micelio (Dinçtürk et al, 2019;Koneman & Allen, 2008).…”