First Report of Potato Blackleg Caused by <i>Pectobacterium carotovorum</i> subsp. <i>brasiliense</i> in Switzerland

Werra, Patrice de; Bussereau, F; Keiser, Andreas; Ziegler, Dain C

doi:10.1094/pdis-07-14-0742-pdn

Cited by 49 publications

(26 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…subsp. brasiliense (Pcb) has also recently emerged (De Werra, Bussereau, & Keiser, ; Duarte, de Boer, Ward, & de Oliveira, ; Gardan, Gouy, Christen, & Samson, ; Hauben et al., ; Khayi et al., ; Nunes Leite et al., ; Samson et al., ; Toth et al., ; van der Wolf et al., ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P carotovorum subsp. carotovorum and Dickeya solani

Lebecka

Flis

Murawska

2018

Journal of Phytopathology

View full text Add to dashboard Cite

The relationship between the rate of in vitro growth of bacterial isolates of Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum and Dickeya solani and their pathogenicity was investigated in tubers of two potato cultivars at four temperatures ranging from 18°C to 30°C. The rate of in vitro growth was highly positively correlated with the number of rotted tubers (r ranged from 0.91 to 0.93) and with the weight of macerated potato tissue, which, however, was only found for P. carotovorum and D. solani (r = 0.76; r = 0.91, respectively) and not for P. atrosepticum. The weight of macerated tissue increased with the temperature, but significant differences between species of bacteria were observed only at 26°C and above, at which temperatures D. solani was the most aggressive, followed by P. carotovorum and P. atrosepticum. Almost all potato tubers inoculated with bacteria showed symptoms of soft rot at 26°C and 30°C, but the number of rotting tubers at lower temperatures (22°C and 18°C) decreased significantly. The lowest disease incidence, 11% of tubers with symptoms, was observed for the D. solani and cultivar Sonda at 18°C, what was also confirmed in a separate experiment with tubers from four potato cultivars inoculated with the highly aggressive isolate of D. solani. At temperatures from 18°C to 30°C, the differences in disease severity between potato cultivars with various resistance to bacteria increased in line with temperature, while the differences in disease incidence decreased.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P carotovorum subsp. carotovorum and Dickeya solani

Lebecka

Flis

Murawska

2018

Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…Van Vaerenbergh, ILVO, Merelbeke, Belgium, personal communication) and the Netherlands (Nunes Leite et al ., ). Recently, the pathogen has also been isolated from blackleg diseased plants in Switzerland (Werra et al ., ), Poland (Waleron et al ., ), England (J. Elphinstone, Fera, York, UK, personal communication), France (V. Hélias, INRA, Rennes, France, personal communication) and Germany (P. E. De Werra, HAFL, Zollikofen, Switzerland, personal communication). It is believed that the pathogen was recently introduced to Europe, as local strains are genetically highly homogeneous (Nunes Leite et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands

Wolf¹,

Haan²,

Kastelein³

et al. 2016

Plant Pathology

View full text Add to dashboard Cite

In western Europe, Pectobacterium carotovorum subsp. brasiliense is emerging as a causal agent of blackleg disease. In field experiments in the Netherlands, the virulence of this pathogen was compared with strains of other Dickeya and Pectobacterium species. In 2013 and 2014, seed potato tubers were vacuum infiltrated with high densities of bacteria (10 6 CFU mL À1 ) and planted in clay soil. Inoculation with P. carotovorum subsp. brasiliense and P. atrosepticum resulted in high disease incidences (75-95%), inoculation with D. solani and P. wasabiae led to incidences between 5% and 25%, but no significant disease development was observed in treatments with P. carotovorum subsp. carotovorum, D. dianthicola or the water control. Co-inoculations of seed potatoes with P. carotovorum subsp. brasiliense and D. solani gave a similar disease incidence to inoculation with only P. carotovorum subsp. brasiliense. However, coinoculation of P. carotovorum subsp. brasiliense with P. wasabiae resulted in a decrease in disease incidence compared to inoculation with only P. carotovorum subsp. brasiliense. In 2015, seed potatoes were inoculated with increasing densities of P. carotovorum subsp. brasiliense, D. solani or P. atrosepticum (10 3 -10 6 CFU mL À1 ). After vacuum infiltration, even a low inoculum density resulted in high disease incidence. However, immersion without vacuum caused disease only at high bacterial densities. Specific TaqMan assays were evaluated and developed for detection of P. carotovorum subsp. brasiliense, P. wasabiae and P. atrosepticum and confirmed the presence of these pathogens in progeny tubers of plants derived from vacuum-infiltrated seed tubers.

show abstract

“…Pectobacterium wasabiae (Nabhan et al 2012;Waleron et al 2013), and Pectobacterium carotovorum subsp. brasiliense (de Werra et al 2015, Nunes Leite et al 2014, Waleron et al 2015. These losses caused by the strains from the mentioned taxa increased significantly in a large number of European countries.…”

mentioning

confidence: 98%

The effect of temperature on the phenotypic features and the maceration ability of Dickeya solani strains isolated in Finland, Israel and Poland

2016

View full text Add to dashboard Cite

Pectinolytic bacteria from the genus Dickeya (former Erwinia chrysanthemi), belonging to Dickeya dianthicola and Dickeya solani species, are causative agents of blackleg and soft rot diseases in Europe. Recently, D. solani have been isolated most frequently from potato plants with the symptoms of blackleg and soft rot. D. solani strains were shown to cause more severe disease symptoms on potato plants than D. dianthicola especially at the higher temperature. They are also able to develop blackleg disease from lower inoculum levels. In the presented study we not only compared phenotypic features of fifteen D. solani strains isolated in countries having different climatic conditions, Poland, Finland and Israel, but also we examined three D. dianthicola strains. The comparison was performed to determine the influence of the strain origin and the temperature of incubation on the ability of the strains to macerate potato tissue and on their major virulence factors such as: pectinolytic, cellulolytic and proteolytic activities, siderophore production and motility. Polish D. solani strains showed higher activities of cell wall degrading enzymes than the Finnish and Israeli strains at all the tested temperatures: 18, 27, 37°C. This observation is correlated with the higher ability of Polish D. solani strains to cause soft rot. In addition, D. solani strains exhibited higher activity of the above mentioned enzymes and caused more severe potato tuber maceration in laboratory tests than the tested D. dianthicola strains. The collected results indicate that although D. solani strains from different climatic conditions have identical Pulse Field Gel Electrophoresis (PFGE) profiles in addition to the same fingerprint profiles obtained by the repetitive sequence-based polymerase chain reaction (REP, ERIC and BOX repetitive sequences), they differ in the examined phenotypic features, especially in the activities of pectinolytic, cellulolytic and proteolytic enzymes and their capacity to macerate potato tuber tissue.

show abstract

First Report of Potato Blackleg Caused by Pectobacterium carotovorum subsp. brasiliense in Switzerland

Cited by 49 publications

References 5 publications

Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P carotovorum subsp. carotovorum and Dickeya solani

Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P carotovorum subsp. carotovorum and Dickeya solani

Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands

The effect of temperature on the phenotypic features and the maceration ability of Dickeya solani strains isolated in Finland, Israel and Poland

Contact Info

Product

Resources

About