First Report of Necrotic Disease Caused by <i>Pantoea agglomerans</i> on Plum (<i>Prunus salicina</i>) in China

Li, Chaohui; Jia, Yixin; Tian, Yun; Zhou, Lan; Sun, Weibo; Deng, Jialin; Liu, Fengquan

doi:10.1094/pdis-10-19-2131-pdn

Cited by 11 publications

(7 citation statements)

References 6 publications

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“…In recent years, it has been gradually found that other bacteria can also cause perforation symptoms in stone fruit trees. For example, Pseudomonas can also cause perforation of leaves and fruits of stone fruit trees 14,16,17 , as can P. agglomerans obtained in this study. This bacterium can also cause perforation symptoms of peach, plum and apricot and is considered to be a new pathogen that can cause bacterial perforation.…”

Section: Discussionsupporting

confidence: 66%

“…In 2020, Tong Yaping and others found a new pathogen of bacterial perforation disease, Pantoea agglomerans, on peach trees in orchards in Fujian Province 16 . In 2020, Li Chaohui and others also isolated P. agglomerans on plum leaves in Dazhou, Sichuan Province, which can lead to perforation symptoms of plum leaves 17 . There are many kinds of pathogens that cause perforation symptoms in stone fruit trees, but there has been no clear conclusion on the types of pathogens causing wild apricot leaf spot-hole disease in the Xinjiang wild fruit forest.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Identification, and Whole-Genome Sequence Analysis of Pantoea Agglomerans Causing Leaf Spot-Hole Disease in Wild Apricot in Xinjiang, China

Liu

et al. 2022

Preprint

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Wild apricot in Yili wild fruit forest in Xinjiang have been seriously affected by leaf spot-hole disease, with the incidence reaching 100%. To identify the pathogen of apricot perforation in the Yili wild fruit forest, two bacterial strains with strong virulence were obtained by the dilution separation method. The bacterial strains were gram-negative bacteria with yellow colonies, smooth surfaces and neat edges. The results of the pathogenicity test showed that the bacteria could cause symptoms of leaf spot-hole disease in wild apricot, similar to the symptoms in the field, and could cause HR in tobacco. Based on the 16S rDNA gene sequence and multilocus sequence analysis of fusA, gyrB, leuS, pyrG, rpoB and rlpB, combined with the physiological and biochemical characteristics, the isolated strain was identified as Pantoea agglomerans. The pathogen causing bacterial leaf spot-hole disease in wild apricot was determined to be P. agglomerans in the wild fruit forest of Yili, Xinjiang. The whole genome of the pathogen strain GL9-2 was sequenced based on the Illumina HiSeq500 and PacBio RS platforms. The genome size was 4765392 bp, and the G+C value was 55.27%. There was one chromosome and two plasmids in the genome, and 4353 CDs were identified. The annotation results showed that 52 glycoside hydrolase-related genes, 38 bacterial secretory system-related genes and 600 toxin-related genes were predicted.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Whole-Genome Sequence Analysis of Pantoea Agglomerans Causing Leaf Spot-Hole Disease in Wild Apricot in Xinjiang, China

Liu

et al. 2022

Preprint

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“…The presence of T. terrea could be associated with vectors, such as Drosophila suzukii [47]. This group of bacteria are usually not related to fruit rot or spoilage, beyond a recent report on P. agglomerans [48]. Overall, the bacteria isolated from damaged cherries had a limited capacity for spoiling tissues around the wounds in individual inoculations.…”

Section: Discussionmentioning

confidence: 89%

Identification of the Causal Agent of Aqueous Spot Disease of Sweet Cherries (Prunus avium L.) from the Jerte Valley (Cáceres, Spain)

Serradilla

Moraga

Ruíz-Moyano

et al. 2021

Foods

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The pre and postharvest disease named ‘aqueous spot’ is an emerging risk for sweet cherries growing in Jerte Valley (Cáceres, Spain). Early stages of the disease appear in the tree, but it is usually detected after harvesting, during the postharvest period. Symptoms include the appearance of skin discolouration and translucency in the shoulder areas. At the most advanced stages, a mycelium of white colour partially or completely covers the fruit. This manuscript provides a detailed description of the microbes involved in this disease, such as bacteria, yeasts, and moulds. Microbes of different cherry cultivars were studied during two consecutive seasons (2019 and 2020). The counts of bacteria and yeast in damaged tissues were higher (7.05 and 6.38 log10 CFU/g for total aerobic mesophilic microbes and yeasts, respectively) than sound tissues (6.08 and 5.19 log10 CFU/g, respectively). The Enterobacterales order dominated the bacteria population. Among yeasts, Yarrowia lipolytica, in 2019, and Metschnikowia pulcherrima and Metschnikowia viticola, in 2020, were consistently isolated from all samples. The presence of moulds was inconsistently detected at the early stage of this disease by plate counts. However, microscopic observations revealed the presence of hyphae in cherry flesh. Different pathogenic moulds were identified, although white mycelium, identified as Botrytis cinerea by molecular methods, was consistently isolated at later stages. Inoculation tests confirmed the involvement of white-mycelium B. cinerea in the development of this new postharvest disease in the Jerte Valley. Its combination with Enterobacterales enhanced the evolution of rotting, whereas the combination with yeasts decreased and delayed the symptoms. This work presents the first report of a consortia of microorganisms implicated in the development of ‘aqueous spot’, an emerging disease in sweet cherry cultivars in the Jerte Valley.

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“…betae causing hyperplasia on both beet and gypsophila (Beer 1991;Cooksey 1986). Additionally, the species has been found to cause symptoms in several cultivated plants (Lee et Chaohui et al, 2020). In Iran, however, P. agglomerans was found to be associated with citrus (Citrus × paradisi Macfad.)…”

Section: Introductionmentioning

confidence: 99%

Pistachio (Pistacia vera L.) canker caused by Pantoea agglomerans

Aeini,

Parizipour,

Nasab

et al. 2023

Eur J Plant Pathol

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Pistachio (Pistacia vera L.) is a perennial woody plant that produces edible seeds with a high nutritional value. Canker disease is one of the important diseases of pistachio, causing considerable loss globally. During 2019-2021, a series of symptoms, including tree weakness, brown to black lesions, canker, and exudation of black sap were observed on the trunk of pistachio trees from different regions of Lorestan province, western Iran. Bacterial isolation resulted in the development of uniform and yellow colonies on yeast-extract peptone glucose agar (YPGA) medium 24 hours post-inoculation (hpi). The colonies were then puri ed on YPGA and yeast-extract-dextrose-calcium-carbonate agar (YDC). Based on the analysis of morphological, biochemical, and molecular characteristics using nucleotide sequences of the gyrB, infB, atpD, and 16S rRNA, a putative pathogen was identi ed as Pantoea agglomerans (formerly Enterobacter agglomerans). To ful ll Koch's postulates, bacterial suspensions (10 7 CFU/ml) were inoculated to one-yearold pistachio seedlings. Thirty days after inoculation, small necrotic lesions developed on the bark in the vicinity of inoculation sites, followed by brown necrotic streaks in the inner bark. Subsequently, the bacterial strains exhibiting the characteristic colony morphology were retrieved from lesions on inoculated pistachio seedlings and veri ed as P. agglomerans. To the best of our knowledge, this is the rst report on the occurrence of pistachio canker caused by P. agglomerans.

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First Report of Necrotic Disease Caused by Pantoea agglomerans on Plum (Prunus salicina) in China

Cited by 11 publications

References 6 publications

Isolation, Identification, and Whole-Genome Sequence Analysis of Pantoea Agglomerans Causing Leaf Spot-Hole Disease in Wild Apricot in Xinjiang, China

Isolation, Identification, and Whole-Genome Sequence Analysis of Pantoea Agglomerans Causing Leaf Spot-Hole Disease in Wild Apricot in Xinjiang, China

Identification of the Causal Agent of Aqueous Spot Disease of Sweet Cherries (Prunus avium L.) from the Jerte Valley (Cáceres, Spain)

Pistachio (Pistacia vera L.) canker caused by Pantoea agglomerans

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