First report of lukM-positive livestock-associated methicillin-resistant Staphylococcus aureus CC30 from fattening pigs in Northern Ireland

Lahuerta‐Marin, Angela; Guelbenzu-Gonzalo, Maria; Pichon, Bruno; Allen, Adrian; Doumith, Michel; Lavery, John; Watson, Conrad; Teale, Christopher; Kearns, Angela

doi:10.1016/j.vetmic.2015.11.019

Cited by 25 publications

(25 citation statements)

References 20 publications

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“…CC30 has previously been found in animals, including camels [21] and pigs [82]. It also has been detected in Spanish white storks [66] and a Portuguese buzzard [68].…”

Section: Resultsmentioning

confidence: 98%

“…aureus strains (LA-MRSA) have emerged, especially in countries with high-density animal farming such as in the Netherlands and in Denmark. LA-MRSA strains belonging to multilocus sequence typing (MLST)-defined clonal complexes (CC) 9 [14,15,79,80,81], CC30 [82] and particularly CC398 [16,17,83,84,85] have spread in several countries and have in some cases also been implicated in human infections.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle-associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock-associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation.

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“…CC30 has previously been found in animals, including camels [21] and pigs [82]. It also has been detected in Spanish white storks [66] and a Portuguese buzzard [68].…”

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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“…For example, the scn gene has been shown to be highly mobile, with phage-mediated loss in an animal host and insertion in a human host potentially occurring in as little as 4 hr (McCarthy et al 2014; Thammavongsa et al 2015). S. aureus spa types that circulate in livestock also can evolve quickly and putative LA- S. aureus markers based on spa types, CCs (e.g., CC398 or CC9) and/or MLSTs may have uncertain specificity depending on regional and microbial selection factors (Bosch et al 2016; Hau et al 2015; Lahuerta-Marin et al 2016; Ye et al 2016b). …”

Section: Discussionmentioning

confidence: 99%

The Prevalence of Antibiotic-Resistant Staphylococcus aureus Nasal Carriage among Industrial Hog Operation Workers, Community Residents, and Children Living in Their Households: North Carolina, USA

Hatcher

Rhodes

Stewart

et al. 2017

Environ Health Perspect

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Background:Antibiotic use in industrial hog operations (IHOs) can support the emergence of antibiotic-resistant (ABR) Staphylococcus aureus. The extent of ABR S. aureus exposure in IHO workers and children living in their households remains unclear.Objective:We investigated ABR S. aureus nasal carriage prevalence among adults with versus without occupational exposure to IHOs and among children living in their households.Methods:In total, 198 IHO worker–child household pairs and 202 community referent (CR) adult–child household pairs completed a questionnaire and provided a nasal swab which was analyzed for S. aureus, methicillin-resistant S. aureus (MRSA), multidrug-resistant S. aureus (MDRSA), absence of scn (putative marker of livestock association), and spa type.Results: S. aureus nasal carriage prevalence was higher among IHO (53%) compared with CR (31%) adults [adjusted prevalence ratio (aPR): 1.40; 95% confidence interval (CI): 1.07, 1.83], but MRSA nasal carriage prevalence was uncommon (2–3%) in IHO and CR adults. MDRSA nasal carriage prevalence was similar among IHO workers and CR adults (12% vs. 8%; aPR: 1.14; 95% CI: 0.56, 2.29). Nasal carriage prevalence was higher among IHO compared with CR children for S. aureus (49% vs. 31%; aPR: 1.50; 95% CI: 1.13, 1.99), MRSA (14% vs. 6%; aPR: 2.37; 95% CI: 1.14, 4.92), and MDRSA (23% vs. 8%; aPR: 2.64; 95% CI: 1.47, 4.75). We also found suggestive evidence of a higher prevalence of S. aureus, MRSA, and MDRSA among children living with an IHO worker who did versus did not report taking personal protective equipment (PPE) home from the IHO. Livestock-associated S. aureus nasal carriage predominated among IHO workers.Conclusion:Our findings support the importance of further research on the prevalence and potential sources of exposure to ABR S. aureus among children living with IHO workers.Citation:Hatcher SM, Rhodes SM, Stewart JR, Silbergeld E, Pisanic N, Larsen J, Jiang S, Krosche A, Hall D, Carroll KC, Heaney CD. 2017. The prevalence of antibiotic-resistant Staphylococcus aureus nasal carriage among industrial hog operation workers, community residents, and children living in their households: North Carolina, USA. Environ Health Perspect 125:560–569; http://dx.doi.org/10.1289/EHP35

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Section: Methodsmentioning

confidence: 97%

“…MRSA were also subjected to WGS using the Illumina HiSeq 2500 platform to determine the lineage (MLST) together with carriage of AMR and virulence genes as described previously (Lahuerta-Marin et al 2016). WGS data were interrogated for lineage-specific canSNPs (Larsen et al 2015), and the phylogenetic relationship between isolates determined at the core genome level by SNP analysis as detailed in Fig 1. Sequencing data have been deposited in the European Nucleotide Archive (ENA) under project number PRJEB18725.…”

Section: Characterization Of Mrsamentioning

confidence: 99%

Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England

Pichon

Wilkinson

Doumith

et al. 2017

Lett Appl Microbiol

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a public health concern worldwide, but has only been reported sporadically in the UK. In the largest UK study to date, samples of raw meat at retail sale were examined for both the presence and levels of MRSA. We report the first isolations of CC398 LA-MRSA from poultry meat in the UK including representatives of a particular sub-clade associated with cases of human infection/colonization in Europe. Although levels were low (<20 CFU per g), the contamination rate was higher than previous UK studies. Moreover, whole-genome sequencing revealed multiple independent introductions of LA-MRSA into the UK food chain.

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First report of lukM-positive livestock-associated methicillin-resistant Staphylococcus aureus CC30 from fattening pigs in Northern Ireland

Cited by 25 publications

References 20 publications

Diversity of Staphylococcus aureus Isolates in European Wildlife

Diversity of Staphylococcus aureus Isolates in European Wildlife

The Prevalence of Antibiotic-Resistant Staphylococcus aureus Nasal Carriage among Industrial Hog Operation Workers, Community Residents, and Children Living in Their Households: North Carolina, USA

Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England

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