First Report of <i>Pectobacterium versatile</i> Causing Blackleg of Potato in Serbia

Marković, Sanja; Komić, Sonja Milić; Jelušić, Aleksandra; Iličić, Renata; Bagi, Ferenc; Stanković, Slaviša; Popović, Tatjana

doi:10.1094/pdis-06-21-1128-pdn

Cited by 11 publications

(11 citation statements)

References 6 publications

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“…A cream-colored, soft mass with a characteristic smell occurred after incubation of tubers infected with P. carotovorum (Table 1). This bacterium (as well as Pectobacterium versatile and Pectobacterium atrosepticum) is also responsible for blackleg disease that causes wilting of the whole plant, and blackening and necrosis of the stem [41]. Another significant bacterial pathogen of potato is Streptomyces scabiei, the cause of common scab.…”

Section: Resultsmentioning

confidence: 99%

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Steglińska

Pielech‐Przybylska²,

Janas³

et al. 2022

Molecules

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The feasibility of early disease detection in potato seeds storage monitoring of volatile organic compounds (VOCs) and plant physiological markers was evaluated using 10 fungal and bacterial pathogens of potato in laboratory-scale experiments. Data analysis of HS-SPME-GC-MS revealed 130 compounds released from infected potatoes, including sesquiterpenes, dimethyl disulfide, 1,2,4-trimethylbenzene, 2,6,11-trimethyldodecane, benzothiazole, 3-octanol, and 2-butanol, which may have been associated with the activity of Fusarium sambucinum, Alternaria tenuissima and Pectobacterium carotovorum. In turn, acetic acid was detected in all infected samples. The criteria of selection for volatiles for possible use as incipient disease indicators were discussed in terms of potato physiology. The established physiological markers proved to demonstrate a negative effect of phytopathogens infecting seed potatoes not only on the kinetics of stem and root growth and the development of the entire root system, but also on gas exchange, chlorophyll content in leaves, and yield. The negative effect of phytopathogens on plant growth was dependent on the time of planting after infection. The research also showed different usefulness of VOCs and physiological markers as the indicators of the toxic effect of inoculated phytopathogens at different stages of plant development and their individual organs.

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Section: Resultsmentioning

confidence: 99%

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Steglińska

Pielech‐Przybylska²,

Janas³

et al. 2022

Molecules

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“…in different combinations with other housekeeping genes (e.g., acnA , gapA , mtlD , pgi , recA , rpoS , etc.) [ 16 , 17 , 18 , 19 , 20 ]. Important parameters when selecting these genes are (i) ubiquity in most enterobacteria, (ii) high discriminatory ability, and (iii) indispensable role in key metabolic processes [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…In their work, Waleron et al [ 19 ] used a combination of nine housekeeping genes ( gapA , gyrA , icdA , pgi , proA , recA , recN , rpoA , and rpoS ) for MLSA of P. zantedeschiae strains isolated from calla lily in central Poland and Serbia. On the other hand, Marković et al [ 20 ] used a combination of genes dnaX , proA, and mdh to identify the causal agents ( P. versatile and P. carotovorum ) of potato blackleg disease in Serbia. The gene dnaX was shown to reliably distinguish between Pectobacterium and Dickeya isolates in several prior studies focusing on the potato blackleg causal agents in different countries [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, P. aroidearum , P. brasiliense , P. odoriferum , P. polaris , and P. wasabiae species have also been reported to result in cabbage soft rot in different countries [ 28 , 29 , 30 , 31 , 32 , 33 ]. In Serbia, different Pectobacterium species (i.e., P. atrosepticum , P. brasiliense, P. carotovorum, P. punjabense, P. zantedeschiae, and P. versatile ) have been isolated from broccoli, calla lily, carrot, celery, parsley, potato, squash, and watermelon [ 19 , 20 , 23 , 34 , 35 , 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia

et al. 2023

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The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.

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“…Therefore, biological activity will be tested on selected pests and pathogens, which can be found in the open field as well as in storage conditions, (i) potato tuber moth Phthorimaea operculella Zeller [32], as one of the most destructive pests of Solanaceae, (ii) gray mold fungi pathogen Botrytis cinerea, that infects fruits (pome, stone), berries, grapes, and several vegetable species [17,30,[33][34][35], and (iii) soft rot bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola destructive on a wide range of plants [36][37][38][39][40]. All of these harmful organisms with broad-host-range are widely present in Serbia and known to cause severe losses on attacked plant hosts.…”

Section: Introductionmentioning

confidence: 99%

Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide

Milićević¹,

Krnjajić

Stević

et al. 2022

Agriculture

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In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite- F-CSZ, nature zeolite- F-CNZ and gelatin- F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.

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First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia

Cited by 11 publications

References 6 publications

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia

Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide

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