First Report of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Associated with Panama Disease of Banana outside Southeast Asia

García-Bastidas, Fernando A.; Ordonez, Nadia; Konkol, Joshua L.; Al-Qasim, M.; Naser, Ziad; Abdel-Wali, M. I.; Salem, Nidà; Waalwijk, C.; Ploetz, R. C.; Kema, G.H.J.

doi:10.1094/pdis-09-13-0954-pdn

Cited by 121 publications

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“…Additionally, this disease is on the move. It has recently been recorded in Pakistan near the Indian border [16], Lebanon [17] and Jordan in the Middle East [18], and Mozambique in Africa [19]. Some tolerant, but not resistant, selections from Cavendish have been reported [20].…”

Section: The Challenges and Opportunities For Future Genetic Improvementmentioning

confidence: 99%

Modifying Bananas: From Transgenics to Organics?

et al. 2017

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Bananas are one of the top ten world food crops. Unlike most other major food crops, bananas are difficult to genetically improve. The challenge is that nearly all banana cultivars and landraces are triploids, with high levels of male and female infertility. There are a number of international conventional breeding programs and many of these are developing new cultivars. However, it is virtually impossible to backcross bananas, thus excluding the possibility of introgressing new traits into a current cultivar. The alternative strategy is to "modify" the cultivar itself. We have been developing the capacity to modify Cavendish bananas and other cultivars for both disease resistance and enhanced fruit quality. Initially, we were using transgenes; genes that were derived from species outside of the Musa or banana genus. However, we have recently incorporated two banana genes (cisgenes) into Cavendish; one to enhance the level of pro-vitamin A and the other to increase the resistance to Panama disease. Modified Cavendish with these cisgenes have been employed in a field trial. Almost certainly, the next advance will be to edit the Cavendish genome, to generate the desired traits. As these banana cultivars are essentially sterile, transgene flow and the outcrossing of modified genes into wild Musa species. are highly unlikely and virtually impossible in other triploid cultivars. Therefore, genetic changes in bananas may be compatible with organic farming.

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Section: The Challenges and Opportunities For Future Genetic Improvementmentioning

confidence: 99%

Modifying Bananas: From Transgenics to Organics?

et al. 2017

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“…Usually, there is considerable time span between the incursion of a plant pathogen and its diagnosis, generating greater economic losses (Stack et al , ). In Jordan, fusarium wilt symptoms on Cavendish plants were first observed in 2006, but TR4 was only diagnosed after eight years (García‐Bastidas et al , ); in the meantime TR4 spread to new farms in the country and some banana farms are now abandoned (Ploetz et al , ). Similarly in Taiwan, TR4 was identified only in 1994 as the strain responsible for wilting in Cavendish observed in the 1960s; now TR4 is widespread in the country (Molina et al , ; Zheng et al , ).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, 50 Fusarium isolates were used (Table ), including one or more isolates per known VCG and 22 TR4 isolates including isolates from Jordan (García‐Bastidas et al , ), Lebanon and Pakistan (Ordóñez et al , ). In addition, 16 other Fusarium spp.…”

Section: Methodsmentioning

confidence: 99%

A loop‐mediated isothermal amplification (LAMP) assay based on unique markers derived from genotyping by sequencing data for rapid in planta diagnosis of Panama disease caused by Tropical Race 4 in banana

et al. 2019

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The socio‐economic impact of Fusarium odoratissimum, which is colloquially called tropical race 4 (TR4), is escalating as this fungal pathogen spreads to new banana‐growing areas. Hence, the development of simple, reliable and rapid detection technologies is indispensable for implementing quarantine measures. Here, a versatile loop‐mediated isothermal amplification (LAMP) assay has been developed that is applicable under field and laboratory conditions. DNA markers unique to TR4 isolates were obtained by diversity arrays technology sequencing (DArTseq), a genotyping by sequencing technology that was conducted on 27 genotypes, comprising 24 previously reported vegetative compatibility groups (VCGs) and three TR4 isolates. The developed LAMP TR4 assay was successfully tested using 22 TR4 isolates and 45 non‐target fungal and bacterial isolates, as well as on infected plants under greenhouse and field conditions. The detection limit was 1 pg µL−1 pure TR4 DNA or 102 copies plasmid‐localized TR4 unique sequence (SeqA) per reaction, which was not affected by background DNA in complex samples. The LAMP TR4 assay offers a powerful tool for the routine and unambiguous identification of banana plants infected with TR4, contributing to advanced diagnosis in field situations and monitoring of fusarium wilt.

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“…It is one among the 120 host-specic, individual strains (formae species) of the wilt pathogen, Fusarium oxysporum (Fox). 16 Besides, aerial dissemination of the pathogens through the infected basil foliage is a major concern. ), in human and animals.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Fusarium wilt in basil (Ocimum sp.) leaves by desorption electrospray ionization mass spectrometry (DESI MS) imaging

et al. 2015

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Basil (Ocimum sp.), a medicinal herb is used fresh and/or dry in various (culinary, cosmetic and pharmaceutical) preparations. Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. basilici is limiting basil cultivation in many countries. Since the leaf is the edible part in basil, new approaches are required to identify, and to prevent the spread of Fusarium pathogens. Desorption electrospray ionization mass spectrometry (DESI MS) was used for imaging thin layer chromatography (TLC)imprints of leaves of three different species of basil (Ocimum basilicum L., O. tenuiflorum L., and O. gratissimum L.), and the molecular manifestations during Fusarium contamination are recorded. DESI MS images showed the chemotaxonomic differences of basil species and the changes in metabolite ion peaks during pathogen infection. Besides easy detection of reported toxic metabolite(s) of the pathogen(s), the results include molecular images showing spatial distribution of all coexisting surfacebound metabolites in plant leaves, their fragment ions, and the transient changes in their spatial distribution during Fusarium attack under natural conditions. Demonstration of the same protocol to image seedling, young/mature leaves, basil/other related plant (Patchouli -Pogostemon cablin (Blanco)Benth.), wilt/other disease symptoms shows that prior knowledge of the metabolite profile of the plant/ pathogen is not required. This rapid detection method can be tailored to large scale screening programs for plant diseases suggesting potential implications in agriculture and quarantine requirements.

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First Report of Fusarium oxysporum f. sp. cubense Tropical Race 4 Associated with Panama Disease of Banana outside Southeast Asia

Abstract: Plant Dis. 98:694, 2014; published online as http:// dx.

Cited by 121 publications

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Modifying Bananas: From Transgenics to Organics?

Modifying Bananas: From Transgenics to Organics?

A loop‐mediated isothermal amplification (LAMP) assay based on unique markers derived from genotyping by sequencing data for rapid in planta diagnosis of Panama disease caused by Tropical Race 4 in banana

Rapid detection of Fusarium wilt in basil (Ocimum sp.) leaves by desorption electrospray ionization mass spectrometry (DESI MS) imaging

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