First report of genotype #65 of Toxoplasma gondii in pigs

Samico-Fernandes, Erika Fernanda Torres; Melo, Renata Pimentel Bandeira de; Kim, Pomy de Cássia Peixoto; Almeida, Jonatas Campos de; Barros, Luiz Daniel de; Garcia, João Luís; Silva, Jean Carlos Ramos; Mota, Rinaldo Aparecido

doi:10.1007/s00436-015-4664-z

Cited by 10 publications

(5 citation statements)

References 31 publications

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“…DNA samples giving positive B1 amplification were then used for genetic characterization. Genotyping was conducted using 11 genetic markers for PCR-RFLP (i.e., SAG1, SAG2, alter.SAG2, SAG3, BTUB, GRA6, c22-8, L358, c29-2, PK1, and Apico) according to previously reported protocol (Yu et al 2013;Qin et al 2014;Cong et al 2015;Samico-Fernandes et al 2015). Nine reference T. gondii strains were included as the positive controls including GT1, PTG, CTG, MAS, TgCgCa1, TgCatBr5, TgWtdSc40, TgCatBr64, and TgToucan (Table 3).…”

Section: Genetic Characterization Of T Gondiimentioning

confidence: 99%

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

et al. 2015

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This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available. The overall prevalence of T. gondii, FIV, FeLV, and D. immitis was 19.34, 9.12, 11.33, and 3.04 %, respectively. For the genetic characterization of T. gondii genotypes in cats, genomic DNA was extracted from the seropositive cats and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genotyped using multilocus PCR-RFLP. Two T. gondii genotypes (ToxoDB#9 and ToxoDB#1) were identified. Results of the multivariate logistic regression analysis showed that older cats are more likely to be seropositive than juveniles for T. gondii, FIV, FeLV, and D. immitis. This is the first report of T. gondii genotypes in cats in northwest China. Moreover, the present study is the first study of retrovirus and D. immitis seroprevalence in cats in China. The results revealed that T. gondii, FIV, and FeLV infections are common in stray and pet cats in northwest China.

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Section: Genetic Characterization Of T Gondiimentioning

confidence: 99%

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

et al. 2015

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“…This genotype has been previously described in cats in Brazil (Pena, Gennari, Dubey, & Su, 2008;Pena et al, 2006). Additionally, a genotype of T. gondii found in chickens, humans, eared doves, and pigs in Brazil (Barros et al, 2014;Dubey et al, 2008;Ferreira et al, 2011;Samico-Fernandes et al, 2015) was characterized as atypical, which is consistent with a previous study that found a high genetic diversity in T. gondii isolates from South America (Shwab et al, 2014). The fact that the same genotype was recorded in different animal species indicates that T. gondii is widely spread around Brazil, infecting different animal species from different regions.…”

Section: Discussionmentioning

confidence: 55%

Occurrence of gastrointestinal protozoans in cats from Londrina, Paraná, Brazil

Torrico

Santos

Abate

et al. 2020

SCA

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Protozoans are widely distributed, and several species may parasitize the digestive tracts of cats (Felis catus), and can be transmitted to humans. The present study aimed to evaluate the species and occurrence of gastrointestinal protozoans in cats in the city of Londrina, Paraná, Brazil. A total of 206 cat fecal samples were tested, of which 141 were from shelter animals, and 65 were from pets owned by local people. Samples were processed by parasitological techniques. Coproparasitological techniques (Willis, Faust and Ziehl-Neelsen) were performed for detection of protozoan parasites. Subsequently, all samples were processed by PCR protocols specific to Toxoplasma gondii, Giardia spp., and Cryptosporidium spp. PCR products from positive samples were selected for sequencing. No samples were found to be positive for Cryptosporidium spp. using the Ziehl-Neelsen technique. Using specific PCR protocols, 1/206 (0.48%) samples tested positive for Cryptosporidium spp. After purification, this one positive sample was sequenced, and it demonstrated a 100% identity match to Cryptosporidium muris. Using specific PCR protocols, 13/206 (9.22%) cat fecal samples tested, including 2/65 (3.08%) pet cat fecal samples, were positive for T. gondii. PCR analysis revealed that 37/206 (17.96%) of cat fecal samples were positive for Giardia spp., including 27/141 (19.15%) of shelter cat fecal samples, and 10/65 (15.38%) pet cat fecal samples (p = 0.5124). When sequenced, these positive samples showed a 100% identity match with Giardia duodenalis. This study demonstrated that infections with Cryptosporidium spp., Toxoplasma gondii, and Giardia duodenalis are present in the population of both pet cats and shelter cats in the city of Londrina. This poses a risk to public health, because these parasites have a high zoonotic potential. ResumoOs protozoários são amplamente distribuídos, e várias espécies podem parasitar o trato digestivo de gatos (Felis catus) e podem ser transmitidas para humanos. O presente estudo teve como objetivo avaliar as espécies e a ocorrência de protozoários gastrintestinais em gatos na cidade de Londrina, Paraná, Brasil.

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“…A total of 57 peer-reviewed published articles studying the prevalence of toxoplasmosis in animals slaughtered in Brazilian abattoirs were found ( Table S2 ) [ 9 , 10 , 11 , 12 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ,…”

Section: Resultsmentioning

confidence: 99%

“…60.3–83.9%) was established, with values ranging from 0 to 73.3%. Numerous studies collected tissues from seropositive pigs and evaluated their infectivity using bioassay with a proportion of positive animals ranging from 2 to 69.2% [ 21 , 61 ] ( Table S6 ). Even though high hygiene standards have been adopted in swine farms in Brazil, outdoor raising still occurs in the country.…”

Section: Discussionmentioning

confidence: 99%

A Systematic Review and Meta-Analysis on the Occurrence of Toxoplasmosis in Animals Slaughtered in Brazilian Abattoirs

Rossi

Costa

Gabriël

et al. 2022

Animals

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Toxoplasmosis is mainly transmitted to human beings through the consumption of contaminated food, and several outbreaks caused by infected meat consumption have been reported in Brazil. We performed a systematic literature review on the prevalence and risk factors for toxoplasmosis in slaughtered animals and performed a meta-analysis of its prevalence for different species and regions. Furthermore, we also discussed the infectivity of seropositive animals, risk factors, and preventive strategies. In the meta-analysis, the overall prevalence estimates for poultry, ostrich, goats, swine, equids, sheep, and bovines were 42.4, 40.4, 23.0, 19.9, 19.1, 17.3, and 16.2%, respectively. Regarding the Brazilian regions, the highest prevalence values were detected for bovines and equids in the South (32.3 and 34.4%, respectively) and swine, goats, sheep, and poultry in the Northeast (29.3, 23.0, 22.9, and 69.8%%, respectively). High proportions of Toxoplasma gondii viability in bioassay conducted on seropositive animals were seen for sheep (34/40 = 85%) and swine (12/15 = 80%). Toxoplasma gondii infections are widespread on Brazilian farms, and the summarized data allow the establishment of high-priority areas and/or species for the adoption of preventive strategies to control this parasite at different levels of the food chain.

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First report of genotype #65 of Toxoplasma gondii in pigs

Cited by 10 publications

References 31 publications

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Occurrence of gastrointestinal protozoans in cats from Londrina, Paraná, Brazil

A Systematic Review and Meta-Analysis on the Occurrence of Toxoplasmosis in Animals Slaughtered in Brazilian Abattoirs

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