First redescription and molecular phylogeny of Trimyema claviforme Kahl, 1933 with the description of a Chinese population of Plagiopyla nasuta Stein, 1860 (Ciliophora, Plagiopylea)

Li, Ran; Zhuang, Wenbao; Feng, Xiaochen; Hines, Hunter N.; Hu, Xiaozhong

doi:10.1016/j.ejop.2023.126003

Cited by 7 publications

(1 citation statement)

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“…The primers 82-F (5′-GAA ACT GCG AAT GGC TC-3′) and ITS-R (5′-TAC TGA TAT GCT TAA GTT CAG CGG-3′) were used for PCR amplifications of the 18S rRNA gene and ITS1-5.8S-ITS2 region (Gao et al 2012 ; Jerome et al 1996 ). To minimize the possibility of PCR amplification errors, Q5 ® Hot Start High-Fidelity 2 × Master Mix DNA Polymerase (New England BioLabs, USA) was used (Li et al 2023 ). The thermal cycler program used was that described by Li et al ( 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Findings on three endocommensal scuticociliates (Protista, Ciliophora) from freshwater mollusks, including their morphology and molecular phylogeny with descriptions of two new species

Li,

Zhang,

Liu

et al. 2024

Mar Life Sci Technol

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Species of the ciliate genera Myxophyllum and Conchophthirus are found as endocommensals of terrestrial and freshwater mollusks, respectively. So far, there have been few studies of these genera and morphological data for most members are often incomplete. In the present work, two new species, Myxophyllum weishanense sp. nov. and Conchophthirus paracurtus sp. nov., and a known species, Conchophthirus lamellidens, were isolated from hosts in Lake Weishan Wetland, China. Taxonomic studies indicate that M. weishanense sp. nov. can be recognized mainly by the combination of about 60 somatic kineties on both ventral and dorsal sides and the presence of caudal cilia. Conchophthirus paracurtus sp. nov. differs from congeners in its body shape and size, having a glabrous area on the posterior right side, and having fewer somatic kineties. In addition, differences in their ITS2 (Internally Transcribed Spacer 2) secondary structures support the discrimination of the two new species from their highly similar congeners. An improved diagnosis for the poorly known species, C. lamellidens is also provided. Phylogenetic analyses reveal that members of the genus Myxophyllum belong to a fully supported clade that is sister to a large, poorly supported clade consisting of Hemispeiridae, Ancistridae, and several lineages of the nonmonophyletic Cyclidiidae. The Myxophyllum clade also includes Protophyra ovicola JQ956552, a possible misidentification. Sequences of the two new Conchophthirus species cluster with other congeners in a fully supported clade that is unrelated to either the ‘typical’ thigmotrichs or to pleuronematids, thus conflicting with the traditional classification, and may represent an orphan scuticociliate lineage.

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