First molecular characterization of plum pox virus strains in stone fruits of Tuscany (Central Italy)

Pedrelli, Athos; Panattoni, Alessandra; Cotrozzi, Lorenzo

doi:10.1007/s42161-023-01430-0

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…TNA was recovered from 500 mg of leaf tissue with cetyltrimethylammonium (CTAB) buffer, according to Li et al [ 52 ] with some modifications [ 53 ]. Briefly, the leaves were powdered in liquid nitrogen with 5 mL CTAB 2% ( w / v ) buffer.…”

Section: Methodsmentioning

confidence: 99%

Physiological and Biochemical Responses Induced by Plum Pox Virus and Plum Bark Necrosis Steam Pitting Associated Virus in Tuscany Autochthonous Plum cv. Coscia di Monaca

Pedrelli,

Ricci,

Panattoni

et al. 2023

Plants

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The present study focused on trees of Tuscany autochthonous plum cv. Coscia di Monaca in order to evaluate the presence of viruses and elucidate the physiological and biochemical responses to virus infections under real field conditions. Among the several investigated viruses, plums tested positive only to plum pox virus (PPV) and plum bark necrosis steam pitting associated virus (PBNSPaV), occurring as both singular and co-infections. This is the first report of PBNSPaV in a Tuscany orchard. Furthermore, the present study not only confirmed the detrimental effects of PPV on the carbon dioxide assimilation rate due to both stomatal limitations and mesophyll impairments, but also showed that although PBNSPaV did not induce such photosynthetic impairments when occurring as singular infection, it enhanced this damaging effect when present as a co-infection with PPV, as confirmed by a severe decrease in the chlorophyll content. Infection-specific responses in terms of accessory pigments (i.e., carotenoids and xanthophylls), as well as sugars and organic acids, were also reported, these being likely related to photoprotective mechanisms and osmotic regulations under virus-induced oxidative stress. Overall, the results here presented represent an important step to fill knowledge gaps about the interaction of plant viruses and autochthonous Prunus cultivars.

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Section: Methodsmentioning

confidence: 99%

Physiological and Biochemical Responses Induced by Plum Pox Virus and Plum Bark Necrosis Steam Pitting Associated Virus in Tuscany Autochthonous Plum cv. Coscia di Monaca

Pedrelli,

Ricci,

Panattoni

et al. 2023

Plants

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The sharka disease on stone fruits in Italy: a review, with a focus on Tuscany

Pedrelli,

Panattoni,

Cotrozzi

2024

Eur J Plant Pathol

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Sharka, caused by plum pox virus (PPV), is by far the most harmful disease of stone fruits (genus Prunus). Although Italy is a major producer of stone fruits, and sharka has spread in most Italian regions since its first identification in the 1970s, information on the diffusion of the disease in Italy results still scattered and incomplete. To address this knowledge gap, the present review aimed to firstly provide a historical overview of sharka in Italy, summarizing outcomes on the disease achieved since the first identification of its causal agent PPV. Data retrieved from a variety of sources (i.e., published literature, official reports and personal communications) showed that (i) sharka spread across almost the whole Italian peninsula as only Liguria, Umbria and Aosta Valley regions officially result disease-free; (ii) the regions with higher rates of infected districts are not always those more dedicated to the production of stone fruits; (iii) only the three major PPV strains causing sharka, i.e., PPV-Dideron (D), PPV-Marcus (M), and PPV-Recombinant (Rec), have been reported in Italy; and (iv) only apricot, peach and plum (i.e., widespread and economically relevant species) are hosts of PPV, with different scenarios among regions. A focus on Tuscany (Central Italy) showed that, (i) sharka has been reported since the 1990s, but extensive PPV monitoring campaigns were run from 2013 to 2021, especially in nurseries; (ii) higher number of symptomatic and PPV positive samples were recorded in farms, mostly in the Arezzo and Florence districts; and (iii) only PPV-M and PPV-Rec strains seem still present. We believe that the present overview will benefit and encourage both researchers for future investigations on sharka and farmers that have to manage the disease.

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First molecular analysis of wisteria vein mosaic virus in Italy: eight new variants reported in Wisteria sinensis

Pedrelli,

Panattoni,

Cotrozzi

2023

J Plant Pathol

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Wisteria vein mosaic virus (WVMV) is a potyvirus infecting Wisteria spp. worldwide, making these largely used ornamental plants unattractive and even unsalable. In 2021, nine Wisteria sinensis plants in Sarzana (Liguria, Italy) showing WVMV symptoms like vein mosaic with irregular patterns, mottling, deformation and twisting margin on leaves were reported. This work describes research on symptomatic and asymptomatic leaves to confirm the identity of the virus infection. All plants tested resulted positive to WVMV. Sequencing of NIb/CP genomic region and comparison on GenBank revealed the presence of eight new genetic variants named Sar 5–12. The eight nucleotide sequences alignments revealed identity ranging between 87.13 and 99.85%. Negative selection (dN/dS < 1) was detected suggesting well adaptation in the area here examined and stability in population. Through a phylogenetic tree, WVMV isolates were grouped in four clades with high bootstrap values, two of which included the eight Italian variants here identified. Since the late 1950 and 1960s, the present study represents the first additional report of WVMV in Italy, as well as its first molecular characterization ever. Further research is required to strengthen our understanding of the movement and transmission of WVMV. Examining how virus infected plants can disseminate this pathogen could help forestall potential risks that neighbouring species may face in their environments.

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First molecular characterization of plum pox virus strains in stone fruits of Tuscany (Central Italy)

Cited by 4 publications

References 39 publications

Physiological and Biochemical Responses Induced by Plum Pox Virus and Plum Bark Necrosis Steam Pitting Associated Virus in Tuscany Autochthonous Plum cv. Coscia di Monaca

Physiological and Biochemical Responses Induced by Plum Pox Virus and Plum Bark Necrosis Steam Pitting Associated Virus in Tuscany Autochthonous Plum cv. Coscia di Monaca

The sharka disease on stone fruits in Italy: a review, with a focus on Tuscany

First molecular analysis of wisteria vein mosaic virus in Italy: eight new variants reported in Wisteria sinensis

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