First Isolation of Dysgonomonas mossii from Intestinal Juice of a Patient with Pancreatic Cancer

Matsumoto, Takehisa; Kawakami, Yoshiyuki; Oana, Kozue; Honda, Takayuki; Yamauchi, Kazuyoshi; Okimura, Yukie; Shiohara, Mayumi; Kasuga, Eriko

doi:10.1016/j.arcmed.2006.05.002

Cited by 20 publications

(12 citation statements)

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“…We detected two species from genus Campylobacter (C. canadensis and C. faecalis ) and both the species were less abundant in Rb3/Rd treated groups. Similarly, Dysgonomonas species have been associated with hepatocellular carcinomas and liver abscess 40 , 41 . Rb3/Rd significantly (p = 0.01) decreased the density of genus Dysgonomonas compared to control group.…”

Section: Resultsmentioning

confidence: 99%

Ginsenosides Rb3 and Rd reduce polyps formation while reinstate the dysbiotic gut microbiota and the intestinal microenvironment in ApcMin/+ mice

Huang

Khan

et al. 2017

Sci Rep

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Studies showed that manipulation of gut microbiota (GM) composition through the treatment of prebiotics could be a novel preventive measure against colorectal cancer (CRC) development. In this study, for the first time, we assessed the non-toxic doses of the triterpene saponins (ginsenoside-Rb3 and ginsenoside-Rd) – as prebiotics – that effectively reinstated the dysbiotic-gut microbial composition and intestinal microenvironment in an ApcMin/+ mice model. Rb3 and Rd effectively reduced the size and the number of the polyps that accompanied with the downregulation of oncogenic signaling molecules (iNOS, STAT3/pSTAT3, Src/pSrc). Both the compounds improved the gut epithelium by promoting goblet and Paneth cells population and reinstating the E-cadherin and N-Cadherin expression. Mucosal immunity remodeled with increased in anti-inflammatory cytokines and reduced in pro-inflammatory cytokines in treated mice. All these changes were correlating with the promoted growth of beneficial bacteria such as Bifidobacterium spp., Lactobacillus spp., Bacteroides acidifaciens, and Bacteroides xylanisolvens. Whereas, the abundance of cancer cachexia associated bacteria, such as Dysgonomonas spp. and Helicobacter spp., was profoundly lower in Rb3/Rd-treated mice. In conclusion, ginsenosides Rb3 and Rd exerted anti-cancer effects by holistically reinstating mucosal architecture, improving mucosal immunity, promoting beneficial bacteria, and down-regulating cancer-cachexia associated bacteria.

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Section: Resultsmentioning

confidence: 99%

Ginsenosides Rb3 and Rd reduce polyps formation while reinstate the dysbiotic gut microbiota and the intestinal microenvironment in ApcMin/+ mice

Huang

Khan

et al. 2017

Sci Rep

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“…Interestingly, the Porphyromonadaceae were 36.3% of the Bacteroidetes, and 12 isolates were similar (91%) to Parabacteroides distasonis, a human gut species that has been shown to convert aromatic amino acids to phenolic acids (41). Another 28 Porphyromonadaceae isolates were similar to Dysgonomonas mossii, which has been isolated from diseased human intestine (42) and microbial fuel cells (43); however, little is known about its function in the intestine.…”

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confidence: 99%

Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

Ziemer

2014

Appl Environ Microbiol

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One of the functions of the mammalian large intestinal microbiota is the fermentation of plant cell wall components. In ruminant animals, the majority of their nutrients are obtained via pregastric fermentation; however, up to 20% can be recovered from microbial fermentation in the large intestine. Eight-week continuous culture enrichments of cattle feces with cellulose and xylan-pectin were used to isolate bacteria from this community. A total of 459 bacterial isolates were classified phylogenetically using 16S rRNA gene sequencing. Six phyla were represented: Firmicutes (51.9%), Bacteroidetes (30.9%), Proteobacteria (11.1%), Actinobacteria (3.5%), Synergistetes (1.5%), and Fusobacteria (1.1%). The majority of bacterial isolates had <98.5% identity to cultured bacteria with sequences in the Ribosomal Database Project and thus represent new species and/or genera. Within the Firmicutes isolates, most were classified in the families Lachnospiraceae, Ruminococcaceae, Erysipelotrichaceae, and Clostridiaceae I. The majority of the Bacteroidetes were most closely related to Bacteroides thetaiotaomicron, B. ovatus, and B. xylanisolvens and members of the Porphyromonadaceae family. Many of the Firmicutes and Bacteroidetes isolates were related to species demonstrated to possess enzymes which ferment plant cell wall components; the others were hypothesized to cross-feed these bacteria. The microbial communities that arose in these enrichment cultures had broad bacterial diversity. With over 98% of the isolates not represented as previously cultured, there are new opportunities to study the genomic and metabolic capacities of these members of the complex intestinal microbiota.A function of the gut microbiota in mammalian herbivores and omnivores is the fermentation of plant cell wall components; mammals lack the enzymes required to breakdown ␤-(1,4) bonds and other linkages between the monosaccharides that make up plant cell walls (1, 2). The plant cell wall is made up of cellulose and hemicelluloses, the primary structural components of plants, and contain smaller and variable amounts of pectins, ␤-glucans, oligosaccharides, lignins, and glycoproteins (3).The physiology of ruminant animals, such as cattle, goats, and sheep, includes a foregut, where fermentation occurs and which allows the animal to directly utilize the short-chain fatty acids (SCFAs) produced by the anaerobic microbial fermentation of plant cell walls for energy (2). Thus, ruminal fermentation provides between 60% and 80% of the animal's energy requirements (4, 5). Ruminants obtain energy from the SCFAs produced in the large intestine, which provides from 0 to 20% of their dietary requirements, similar to the levels reported for other mammals (4, 5).A number of intestinal microbial metagenomes, including those of cattle (6, 7), human (8, 9), pig (10), and chicken (11), have been demonstrated to be enriched in carbohydrate transport and metabolism genes. Ley et al. (1) found that the type of diet (herbivorous, omnivorous, or carnivorous) strongly predic...

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“…The genus Dysgonomonas is a gram-negative anaerobe which can be isolated from the human intestine [35], brain abscesses [36], and bronchoalveolar lavage fluid (BALF) from COPD patients [14]. Few reports have characterized D .…”

Section: Discussionmentioning

confidence: 99%

16S rDNA analysis of periodontal plaque in chronic obstructive pulmonary disease and periodontitis patients

Chen

et al. 2017

Journal of Oral Microbiology

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This study investigated if chronic obstructive pulmonary disease (COPD) is correlated with periodontitis via periodontal microbiota and if certain bacteria affect periodontitis as well as COPD. Moreover, the study investigated whether suffering from COPD is associated with a decrease in the richness and diversity of periodontal microbiota. Subgingival plaque was obtained from 105 patients. Bacterial DNA was isolated from 55 COPD and 50 non-COPD participants (either with or without periodontitis). 16S rRNA gene metagenomic sequencing was used to characterize the microbiota and to determine taxonomic classification. In the non-periodontitis patients, suffering from COPD resulted in a decrease in bacteria richness and diversity in the periodontal microenvironment. An increase in the genera Dysgonomonas, Desulfobulbus, and Catonella and in four species (Porphyromonas endodontalis, Dysgonomonas wimpennyi, Catonella morbi, and Prevotella intermedia) in both COPD and periodontitis patients suggests that an increase in these periodontitis-associated microbiota may be related to COPD. Three genera (Johnsonella, Campylobacter, and Oribacterium) were associated with COPD but not with periodontitis. The decrease in the genera Arcanobacterium, Oribacterium, and Streptomyces in COPD patients implies that these genera may be health-associated genera, and the decrease in these genera may be related to disease. These data support the hypothesis that COPD is correlated with periodontitis via these significantly changed specific bacteria.

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First Isolation of Dysgonomonas mossii from Intestinal Juice of a Patient with Pancreatic Cancer

Cited by 20 publications

References 5 publications

Ginsenosides Rb3 and Rd reduce polyps formation while reinstate the dysbiotic gut microbiota and the intestinal microenvironment in ApcMin/+ mice

Ginsenosides Rb3 and Rd reduce polyps formation while reinstate the dysbiotic gut microbiota and the intestinal microenvironment in ApcMin/+ mice

Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

16S rDNA analysis of periodontal plaque in chronic obstructive pulmonary disease and periodontitis patients

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