First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

Wasniewski, Marine; Almeida, Isabel Tavares de; Baur, Andreas S.; Bedeković, Tomislav; Boncea, D.; Chaves, Luciana Botelho; David, Dan; Benedictis, Paola De; Dobrostana, M; Giraud, P; Hostnik, Peter; Jacevičienė, Ingrida; Kenklies, S.; König, Melanie; Mähar, K.; Mojžíš, Miroslav; Moore, Susan M.; Mrenoski, S.; Müller, Thomas; Ngoepe, Ernest; Nishimura, Masaaki; Nokireki, Tiina; Pejovic, Nikolai; Smreczak, Marcin; Strandbygaard, Bertel; Wodak, Eveline; Cliquet, Florence

doi:10.1016/j.jviromet.2016.10.006

Cited by 21 publications

(21 citation statements)

References 34 publications

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“…The seroprevalence in this study assessed with the ELISA is in the range of results from other European studies conducted with mFAVN or RFFIT, thus indicating the principle applicability of this approach for the detection of lyssavirus antibodies in bat samples in the frame of passive rabies surveillance and serological surveys (for a review, see Reference [16]). An unspecific binding of serum components that would lead to false positive results in the ELISA cannot be excluded; however, such a scenario was not reported during the broad usage of this ELISA in foxes or raccoon dogs [39]. This was corroborated by analyzing Eptesicus serotinus and Eptesicus fuscus sera from experimental studies.…”

Section: Discussionmentioning

confidence: 91%

“…Obviously, a stringent validation, as was done in the development of the ELISA to be used for foxes and raccoon dogs for post-vaccination monitoring [39], is lacking for bats. Against the background of the genetic diversity of bats, the protected status of European bats, and the need for adaptation of cut-offs per species [47], this is rather illusionary.…”

Section: Discussionmentioning

confidence: 99%

“…Antibodies against lyssaviruses were detected using the commercially available BioPro Rabies ELISA Ab kit (O.K. SERVIS BioPro, Prague, Czech Republic), a blocking ELISA for the serological detection of rabies antibodies in domesticated [38] and wild animals [39]. In brief, following manufacturer's instruction on the modification of the ELISA for bat sera, 10 µL of bat sample was diluted 10-fold to a total of 100 µL using the sample diluent buffer provided in the kit.…”

Section: Detection Of Lyssavirus Antibodies With Bio-pro Rabies Elisamentioning

confidence: 99%

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Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Orłowska

Smreczak

Freuling

et al. 2020

Viruses

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Background: Bats are known to host a number of nonpathogenic viruses, as well as highly pathogenic viruses causing fatal diseases like rabies. Serological surveys as part of active and passive bat rabies surveillance mainly use seroneutralization assays, demonstrating the presence of lyssavirus-specific antibodies in a variety of European bats, particularly against European bat lyssaviruses type 1 (EBLV-1). Here, we present the first serological survey in European bats of this kind during which European bats from Poland collected in the frame of passive rabies surveillance between 2012 and 2018, as well as Serotine bats (Eptesicus serotinus) and North American Big Brown bats (Eptesicus fuscus) from previous experimental studies, were tested using a commercial ELISA kit for the detection of anti-lyssavirus antibodies. Results: Lyssavirus-specific antibodies were detected in 35 (30.4%) out of 115 Polish bats of both sexes, representing nine out of 13 identified bat species endemic mainly to Central Southern Europe and Western Asia, i.e., Eptesicus serotinus, Nyctalus noctula, Myotis daubentonii, Plecotus auritus, Vespertillo murinus,Pipistrellus pipistrellus, Pipistrellus pipilstrellus/Pipistrellus pygmaeus, Myotis brandtii, and Barbastella barbastellus. Seroprevalence was highest in bat species of Nyctalus noctula, Eptesicus serotinus, Plecotus auritus, and Myotis daubentonii. More than 60% of the ELISA seropositive bats originated from the voivodeships of Silesia, Lower-Silesian, Warmian-Mazurian, and Mazowian. Rabies-specific antibodies were also found in Eptesicus fuscus bats from North America. Conclusions: The study demonstrates the principal application of the BioPro Rabies ELISA Ab Kit for the detection of anti-lyssavirus specific antibodies in body fluids and serum samples of bats. However, results may only be reliable for North American bats, whereas interpretation of results for European bats per se is difficult because proper validation of the test is hampered by the protected status of these species.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Detection Of Lyssavirus Antibodies With Bio-pro Rabies Elisamentioning

confidence: 99%

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Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Orłowska

Smreczak

Freuling

et al. 2020

Viruses

View full text Add to dashboard Cite

show abstract

“…This panel of serum samples was tested with an ELISA kit (BioPro rabies ELISA Ab kit, Prague, Czech Republic), a blocking ELISA for serological diagnostic of rabies lyssavirus antibody in serum or plasma of domesticated and wild animals [ 16 ]. In brief, the serum samples and controls (rabies positive control serum, rabies control serum 1, rabies control serum 2, rabies control serum 1 and rabies negative control) were diluted two-fold using sample diluent buffer provided in the kit.…”

Section: Methodsmentioning

confidence: 99%

Sero-Surveillance of Lyssavirus Specific Antibodies in Nigerian Fruit Bats (Eidolon helvum)

et al. 2017

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The aetiological agent of rabies is a member of the Lyssavirus genus (Rhabdoviridae family, order Mononegavirales). The disease (rabies) is endemic in many parts of Asia and Africa and still remains an important public and veterinary health threat. In Nigeria, there is a dearth of information on the natural infection and/or exposure of bat species to lyssaviruses. Therefore, this study was undertaken to assess the prevalence of rabies virus (RABV) neutralizing antibodies in sera obtained from bats from the central Plateau and North-East Bauchi States in Nigeria. Two hundred serum samples were collected from Nigerian fruit bats from six different locations and tested for anti-RABV antibodies using a commercial blocking ELISA. Of the 200 bat serum samples collected, one batch consisting of 111 samples did not meet the validation criteria and hence was not included in the final analysis. Of the remaining 89, only three (3.4%) contained anti-lyssavirus antibodies, demonstrating a low prevalence of lyssavirus antibodies in the study population. In order to further understand the exposure of bat species to phylogroup II lyssaviruses (Lagos bat virus and Mokola virus), the same panel of samples will be tested for neutralizing antibodies to phylogroup II members, viruses that do not cross-neutralize with members of phylogroup I.

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“…It is easier to be performed than gold standard FAVN or RFFIT. Recent developments had prompted out others ELISA kits with satisfactory performance [13].…”

Section: Methodsmentioning

confidence: 99%

Assessment of anti Rabies post-vaccinal immunity in domestic carnivores pets in low resources setting

Koffl¹,

Kouakou

et al. 2020

Preprint

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Objective Rabies is a zoonotic disease transmissible to humans mainly by dogs. It is a vaccine preventable disease. In Ivory Coast, canine rabies is a prioritary and notifiable animal disease since 1962. Evaluation of post-vaccination immunity against rabies was undertaken between 2003 and 2009 by an immunoenzymatic test Platelia Rabies II ELISA Kits (Bio-Rad). We aim to investigate anti Rabies vaccination efficacy in Ivory Coast, using data obtained purposely for antibody quantification prior to international movement of pets. Results A total of 527 dogs and cats sera were analyzed. Generally cats had developed higher level of post vaccinal immunity than dogs. We have found that more than 95.80% (114/119) of cats had a serum with antibodies titer above 0.5 EU /ml so like 74.75% (n = 305) of dogs. Undetectable or insufficient level of seroconversion was noted either in the canine specie (25.25%) or in cats (4.2%). Multivaccined Dogs, older than 4 years, represented the most important group (62.25%) of seronegative dog population. Associated with vaccination, serological results were used as data based-evidence in compliance with official requirements governing free movements of pets from rabies-infected to rabies-free european countries. Keywords : Ivory Coast, Rabies, ELISA, post-vaccinal immunity, dogs, cats

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First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

Cited by 21 publications

References 34 publications

Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Sero-Surveillance of Lyssavirus Specific Antibodies in Nigerian Fruit Bats (Eidolon helvum)

Assessment of anti Rabies post-vaccinal immunity in domestic carnivores pets in low resources setting

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