First evaluation of six nucleic acid amplification tests widely used in the diagnosis of Chlamydia trachomatis in Russia

Abstract

According to the reference methods, C. trachomatis NAATs developed and used in Russia have relatively good performance characteristics for both invasive and non-invasive samples. However, larger studies that include symptomatic and asymptomatic patients as well as genital and extra-genital samples, and in comparison with other internationally well-recognized, validated, and ideally Food and Drug Administration-approved C. trachomatis NAATs performed strictly according to the manufacturer's instructions, need to be conducted.

Can the Swedish new variant of Chlamydia trachomatis (nvCT) be detected by UK NEQAS participants from seventeen European countries and five additional countries/regions in 2009?

“…Furthermore, no countries in eastern Europe were represented. In several of these countries, there are many shortcomings in the diagnosis of C. trachomatis and use of internationally available commercial NAATs is rare [11,12]. Some of the nationally produced and in house NAATs that are in use for diagnosis of C. trachomatis [11] may have their target in the nvCT plasmid deletion.…”

An Algorithm of a Laboratory Examination of Patients for Neisseria Gonorrhoeae, Chlamydia Trachomatis, Mycoplasma Genitalium and Trichomonas Vaginalis Infections Using the Polymerase Chain Reaction and the Transcriptional Amplification Reaction

“…Это согласуется с результатами иссле-дования C. Black и соавт. [25], в котором чувстви-тельность метода культивирования хламидий соста- [18,19,26]. Для диагностики инфекции, вызванной M. genitalium, в настоящее время МБМ являются безаль-тернативными из-за крайне малых размеров микро-организма и трудностей культивирования.…”

Guidelines for the laboratory diagnosis of Chlamydia trachomatis infections in East European countries

“…present time, only one validation study has been conducted in Eastern Europe, following these principles. 43 Based on these principles, for validation of a new test for chlamydial infection, we recommend:…”

“…41 Furthermore, these variants have not yet been detected in Eastern Europe, although there have been only limited studies. 42,43 Strand displacement amplification: the Becton Dickinson BD ProbeTec ET General…”