First Detection of New Delhi Metallo-β-Lactamase-5-Producing<i>Escherichia coli</i>from Companion Animals in Korea

Hong, Jun; Song, Wonkeun; Park, Hee Myung; Oh, Jae Young; Chae, Jong Chan; Han, Jae Ik; Jeong, Seok Hoon

doi:10.1089/mdr.2018.0237

Cited by 28 publications

(18 citation statements)

References 27 publications

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“…In a recent study from Finland, bla NDM‐5‐ harbouring E. coli designated to ST167 has been identified in both humans and dogs (Grönthal et al, ). Meanwhile, bla NDM‐5 that belonged to ST410 was recently reported from dogs in South Korea (Hong et al, ) and the UK (Reynolds et al, ). In Egypt, E. coli ST410, co‐harbouring both bla NDM‐5 and bla OXA‐181 , has been identified from patients (Gamal et al, ).…”

Section: Discussionmentioning

confidence: 95%

“…However, the first identification of bla NDM‐5 E. coli from dogs in Northern Africa was reported in Algeria (Yousfi et al, ). Other studies have recently reported the isolation of bla NDM‐5 E. coli from dogs in Finland (Grönthal et al, ), South Korea (Hong et al, ) and the UK (Reynolds et al, ). Our analysis showed various STs linked to different NDM allelles in E. coli isolates from both sources.…”

Section: Discussionmentioning

confidence: 98%

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Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Ramadan

Gupta

Sharma

et al. 2019

Zoonoses and Public Health

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The emergence of NDM-producing Escherichia coli has considerably threatened human and animal health worldwide. This study describes for the first time in Egypt, the draft genome sequences of emerging NDM-5-producing E. coli from humans and dogs, and investigates genetic relatedness between isolates from both sources. Two E. coli from human urine and seven from environmental clinical samples of dogs exhibited resistance to carbapenems and harbouring bla NDM were subjected to Illumina Miseq whole-genome sequencing (WGS). Assembly and analysis of the reads were performed to identify resistance genes, multilocus sequence types (MLST), plasmid replicon types (Inc) and insertion sequences (IS) of the bla NDM region; core genome MLST (cgMLST) analysis was also performed. Two different NDM alleles were identified; bla NDM-5 in E. coli HR119 from the urine of a healthy person and environmental samples of dogs, and bla NDM-1 in E. coli HR135 from a human patient's urine. Multiple mobilizable resistance genes to different antimicrobial classes were identified except the colistin resistance gene, mcr. E. coli isolates from humans and dogs were assigned to different sequence types (STs). Using cgMLST, dog isolates clustered together with only 1-2 allellic differences; however, human E. coli showed 1,978 different allelles compared with dog isolates. Plasmidfinder results indicated the presence of an IncX3 replicon in bla NDM-5 -producing E. coli; however, bla NDM-1 was linked to IncCoIKP3.Notably, the NDM region (3 Kb) in all isolates from humans and dogs was highly similar with variable flanking sequences that represented different IS elements. This study reports the first emergence of NDM-5-producing E. coli from dogs in Egypt that shared some genetic features with human isolates and could be considered potential public health threats. K E Y W O R D S Egypt, Escherichia coli, insertion sequences, NDM-5, plasmid replicon, whole-genome sequencing | 325 RAMADAN et Al. This whole-genome shotgun project has been deposited at DDBJ/ ENA/GenBank under BioProject no. (PRJNA489683). ACK N OWLED G EM ENTSThe authors woulk like to thank the veterinarians in Zoo Care and Pet Clinic, Mansoura, Egypt, for their help during sample collection from animals. CO N FLI C T S O F I NTE R E S TNone. D ECL A R ATI O NThe mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information | 329 RAMADAN et Al. and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. O RCI D Hazem Ramadanhttps://orcid.org/0000-0002-6687-3758Charlene R. Jackson https://orcid.org/0000-0001-8480-4556 S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Ramadan

Gupta

Sharma

et al. 2019

Zoonoses and Public Health

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“…All of the 1205 E. coli isolates were also susceptible to MEM (data not shown). Carbapenem-resistant E. coli have not yet been reported in food-producing animals and meat products, whereas carbapenem-resistant E. coli have been identified from human samples and companion animals in previous studies in Korea [23,57,58]. All of ESBL-ECs in this study also showed the multidrug resistance phenotype.…”

Section: Discussionmentioning

confidence: 47%

“…Since whole-genome sequencing (WGS) has become affordable and facilitates the acquisition of useful information regarding multiple AMR genes, genomic mutations, and higher-resolved microbial typing from a single assay, some countries, including the USA, have already conducted WGS-based AMR surveillance [22]. A number of studies have reported the prevalence and characteristics of ESBL-EC from humans and food-producing animals in Korea because of the importance of such ESBL-ECs from a public health perspective [23][24][25][26][27][28][29]. However, few studies have reported the prevalence and characteristics of ESBL-ECs in retail meat in Korea [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea

Kim

et al. 2020

Microorganisms

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The spread of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) has posed a critical health risk to both humans and animals, because resistance to beta-lactam antibiotics makes treatment for commonly infectious diseases more complicated. In this study, we report the prevalence and genetic characteristics of ESBL-ECs isolated from retail meat samples in Korea. A total of 1205 E. coli strains were isolated from 3234 raw meat samples, purchased from nationwide retail stores between 2015 and 2018. Antimicrobial susceptibility testing was performed for all isolates by a broth microdilution method, and the ESBL phenotype was determined according to the Clinical and Laboratory Standards Institute (CLSI) confirmatory method. All ESBL-EC isolates (n = 29) were subjected to whole-genome sequencing (WGS). The antimicrobial resistance genes, plasmid incompatibility types, E. coli phylogroups, and phylogenetic relations were investigated based on the WGS data. The prevalence of ESBL-ECs in chicken was significantly higher than that in other meat samples. The results in this study demonstrate that clonally diverse ESBL-ECs with a multidrug resistance phenotype were distributed nationwide, although their prevalence from retail meat was 0.9%. The dissemination of ESBL-ECs from retail meat poses a potential risk to consumers and food-handlers, suggesting that the continuous surveillance of ESBL-ECs in retail meat should be conducted at the national level.

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“…The possible way companion animals may get infected with CRE is through direct contact with colonized hosts and contaminated environment. Eventually, companion animal may become a reservoir for CPE [ 62 , 63 , 64 , 65 ]. For instance, a bla OXA-48 carbapenemase-producing K. pneumoniae has been transmitted from human to companion animals (dogs) through contaminated hands [ 66 ].…”

Section: Companion Animalsmentioning

confidence: 99%

Molecular Epidemiology of Carbapenemases in Enterobacteriales from Humans, Animals, Food and the Environment

et al. 2020

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The Enterobacteriales order consists of seven families including Enterobacteriaceae, Erwiniaceae, Pectobacteriaceae, Yersiniaceae, Hafniaceae, Morganellaceae, and Budviciaceae and 60 genera encompassing over 250 species. The Enterobacteriaceae is currently considered as the most taxonomically diverse among all seven recognized families. The emergence of carbapenem resistance (CR) in Enterobacteriaceae caused by hydrolytic enzymes called carbapenemases has become a major concern worldwide. Carbapenem-resistant Enterobacteriaceae (CRE) isolates have been reported not only in nosocomial and community-acquired pathogens but also in food-producing animals, companion animals, and the environment. The reported carbapenemases in Enterobacteriaceae from different sources belong to the Ambler class A (blaKPC), class B (blaIMP, blaVIM, blaNDM), and class D (blaOXA-48) β-lactamases. The carbapenem encoding genes are often located on plasmids or associated with various mobile genetic elements (MGEs) like transposons and integrons, which contribute significantly to their spread. These genes are most of the time associated with other antimicrobial resistance genes such as other β-lactamases, as well as aminoglycosides and fluoroquinolones resistance genes leading to multidrug resistance phenotypes. Control strategies to prevent infections due to CRE and their dissemination in human, animal and food have become necessary. Several factors involved in the emergence of CRE have been described. This review mainly focuses on the molecular epidemiology of carbapenemases in members of Enterobacteriaceae family from humans, animals, food and the environment.

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First Detection of New Delhi Metallo-β-Lactamase-5-ProducingEscherichia colifrom Companion Animals in Korea

Cited by 28 publications

References 27 publications

Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea

Molecular Epidemiology of Carbapenemases in Enterobacteriales from Humans, Animals, Food and the Environment

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