First detection of bla TEM, SHV and CTX-M among Gram negative bacilli exhibiting extended spectrum -lactamase phenotype isolated at University Hospital Center, Yalgado Ouedraogo, Ouagadougou, Burkina Faso

Abstract

Resistance to a wide variety of common antimicrobials is observed among clinical strains designed as extended spectrum β-lactmase (ESBL) producers. They produce enzymatic protein which inactivates efficiently oxyimino cephalosporin and constitutes a serious global health concern that has complicated treatment strategies. Many studies report high prevalence of ESBL producers among Gram negative bacilli. The aim of this work was to identify the presence of TEM, SHV and CTX-M families in these strains which were initially screened by phenotypic method. Gram negative bacilli resisting third or four generation cephalosporin were isolated during anti-biogram study. The presence of ESBL positivity was detected using the double disk synergy test. Minimal inhibitory concentrations (MICs) of ceftriazon for any strain were determined using E-test manufacturing protocol. Polymerase chain reaction (PCR) analysis for β-lactamase (bla) genes of TEM, SHV and CTX-M family was carried out using designed primers in 171 ESBL isolates producers. Among 259 Gram negative bacilli collected, 171 (66, 02%) exhibited ESBL producers' profile. Urine samples constitute major source of ESBL producers. The highest prevalence of ESBL was observed in Escherichia coli (75, 50%). Among ESBL isolates producers, gene prevalence of bla-CTX-M (65, 49%) was highest, followed by bla-TEM (25, 73%) and bla-SHV (18, 71%) in the present study. The frequency of ESBL producing strains among clinical isolates has been steadily increased. Continual drug resistance surveillance and molecular characteristics of ESBL isolates are necessary to guide the appropriate and judicious antibiotic use.

First Sequence Analysis of Genes Mediating Extended-Spectrum Beta-Lactamase (ESBL) bla-TEM, SHV- and CTX-M Production in Isolates of Enterobacteriaceae in Southern Benin

“…The bacteria that showed higher levels of resistance were Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae. The same observation was previously made in Burkina Faso (27,28).…”

“…Kamga et al (33) observed a strong presence of TRI (inhibitor-resistant TEM) in Escherichia coli strains, which may justify the high presence of TEM in the Escherichia coli strains isolated in the present study. Zongo et al (28) in Burkina Faso, Salah (34), and Diagbouga et al (35) in Togo also found that the bla-Tem gene was the most widespread in ESBL-producing Escherichia coli strains, whereas Bla-SHV was the predominant gene in ESBL-producing Klebsiella pneumonia strains. However, the high proportion of bla-CTX-M in Togo (95.7%) and Burkina Faso (65.49%) is not the same as in our study because this gene came second after bla-Tem and represented only 18%.…”

The Prevalence of blaCTX-M, blaTEM Genes in Bacteria Isolated From Bladder Cancer Patients with UTI

“…(17.65%), and E. coli (14.71%), followed by S. aureus (13.72%).The screening of ESBL producers in our finding was done by Double Disc Synergy Test. The total ESBLs producers in the result of the recent study were Similar to that reported in other studies : (29) ; (30); (31) ,the disagreement with our finding reported by (32). The highest ESBLs Producer in all Groups was E.coli .…”

Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates Obtained from a Tertiary Hospital in Gombe Nigeria

“…According to Zongo et al [51], the coproduction of all the three genes (TEM + SHV + CTX-M) was 10.52% prevalent in the samples they studied in Burkina Faso, and this low figure seems to tally with the 10% and 5% prevalence seen in this work in K. pneumonia and E. coli respectively. Similar result was also reported by [18] in which all the three genes carried 9.09% prevalence.…”