First cytogenetic studies of the genus <i>Heptapterus</i> (Actinopterygii, Siluriformes): karyotype differentiation and review of cytogenetic data on the Heptapteridae family

Yano, Cássia Fernanda; Margarido, Vladimir Pavan

doi:10.1111/j.1095-8649.2012.03314.x

Cited by 5 publications

(6 citation statements)

References 48 publications

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“…However, another scenario can also not be ruled out, in which fission-type rearrangements would have originated the additional 12 acrocentric chromosomes in karyomorph A. Once the majority of chromosomal rearrangements involve heterochromatic regions, especially in fish species [26, 27], this explains the centromeric rearrangements in M. albus . The co-localization of CMA 3 positive heterochromatin with the NOR loci also occurs in other synbranchids [25, 28], probably because of local changes in base composition (increase in GC content) due to the so-called GC-biased gene conversion that involves rDNA in many vertebrates, including ray-finned fishes [29].…”

Section: Discussionmentioning

confidence: 99%

Karyotype diversity and evolutionary trends in the Asian swamp eel Monopterus albus (Synbranchiformes, Synbranchidae): a case of chromosomal speciation?

et al. 2019

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Background Synbranchidae or swamp eels are fishes belonging to the order Synbranchiformes that occur in both freshwater and occasionally in brackish. They are worldwide distributed in tropical and subtropical rivers of four different continents. A large degree of chromosomal variation has been found in this family, mainly through the use of conventional cytogenetic investigations. Inside this group, a still almost unexplored species under the cytogenetic point of view is the Asian swamp eel Monopterus albus , a widely distributed species throughout Asia. Here, we tested the hypothesis of chromosomal speciation, where a case of sympatric speciation may occur as the primary consequence of chromosomal rearrangements. We performed a comparative chromosomal analysis of M. albus from 22 different localities in Thailand, using distinct staining methods (C-banding, Ag-NO 3, and Chromomycin A 3 ), and FISH with repetitive DNA probes (5S rDNA, 18S rDNA, Rex1 element and microsatellite repeats). Results This approach evidenced two contrasting karyotypes (named karyomorphs A and B) that varied concerning their 2n and repetitive DNAs distribution, where chromosomal fusions and pericentric inversions were involved in such differentiation. While the karyomorph A has 2n = 24 chromosomes, the karyomorph B has only 2n = 18, both with NF = 24. In addition, karyomorph A contains only acrocentric chromosomes, while karyomorph B contains three unique metacentric pairs. These features highlight that M. albus has already gone through a significant genomic divergence, and may include at least two cryptic species. Conclusions This marked chromosomal differentiation, likely linked to the lifestyle of these fishes, point to the occurrence of a chromosomal speciation scenario, in which fusions and inversions had a prominent role. This highlights the biodiversity of M. albus and justifies its taxonomic revision, since this nominal species may constitute a species complex.

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Section: Discussionmentioning

confidence: 99%

Karyotype diversity and evolutionary trends in the Asian swamp eel Monopterus albus (Synbranchiformes, Synbranchidae): a case of chromosomal speciation?

et al. 2019

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“…Almost all Heptapteridae species show nucleolar organizers regions (NORs) on a single chromosome pair, situation confirmed by means of in situ hybridization with rDNA 18S probes in 24 species, only Heptapterus mustelinus 9,13 and Pimelodella sp. 14 show multiple NORs.…”

Section: Introductionmentioning

confidence: 83%

“…However, species of the Pseudopimelodidae family are characterized by stability in chromosome numbers showing almost all 2n = 54 chromosomes. [7][8][9][10][11][12] Heptapteridae shows 218 species and 32 genera and Pseudopimelodidae 50 species and 9 genera 2 ; however, the karyotypic data are relatively scarce, especially those related with ribosomal DNAs, and only available for some genera (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Trends in Two Families of the Neotropical Catfishes: Heptapteridae and Pseudopimelodidae (Siluriformes)

et al. 2018

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Several neotropical Siluriformes groups suffered important taxonomic revisions based on the evaluation of morphological and molecular characteristics that allow the construction of new phylogenetic hypothesis. In the present study were cytogenetically analyzed six species belonging to Heptapteridae (Cetopsorhamdia iheringi, Phenacorhamdia tenebrosa, Rhamdella eriarcha, Pimelodella meeki, Pimelodella australis, Heptapterus mustelinus) and two to Pseudopimelodidae families (Microglanis cottoides and Microglanis cibelae) by means of differential staining techniques to describe more precisely cytogenetic similarities and differences. The diploid number of R. eriarcha with 2n = 58 and M. cibelae with 2n = 56 were reported for the first time. Also, the lowest chromosome number (2n = 48) for P. tenebrosa was described. The chromosome-banding techniques for to put in evidence nucleolar organizers impregnated by silver nitrate ([AgNORs], chromomycin A [CMA], and rDNA 18S) showed for all studied species conserved patterns, characteristic for each family. The rDNA 5S showed high variability among species or populations of both families, these regions could be simple or multiple, syntenic, or not with rDNA18S. The chromosome markers showed that both families are related not only from a morphologic point of view but also by their karyotypic characteristics, however, some of the present cytogenetic results evidence the importance of new morphologic, molecular, and phylogenetic studies to improve the knowledge of these fish groups.

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“…The species Phenacorhamdia tenebrosa (Schubart, 1964), which belongs to the Nemuroglanis subclade, also has 2n = 58 (Borba et al 2011), but no interstitial NOR. Since 2n = 58 is considered the basal number for Heptapteridae (Fenocchio et al 2003, Borba et al 2011), and the species Imparfinis borodini (Mees & Cala, 1989) (Vissoto et al 1999), Imparfinis hollandi (Margarido and Moreira-Filho 2008) and Heptapterus mustelinus (Valenciennes, 1835) (Yano and Margarido 2012) have a reduced diploid number (2n = 52, 2n = 42 and 2n = 54, respectively), it is possible that Robertsonian translocations were responsible for the karyotypic changes.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, only the genera Imparfinis , Cetopsorhamdia , Heptapterus , Phenacorhamdia , Rhamdiopsis , Pimelodella , Rhamdia , and Taunayia had been cytogenetically analyzed; these represent only eight of the 24 genera in the family Heptapteridae (Yano and Margarido 2012). The first five belong to the subclade Nemuroglanis .…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic study of heptapterids (Teleostei, Siluriformes) with particular respect to the Nemuroglanis subclade

Kantek¹,

Peres²,

Moreira‐Filho³

2015

CCG

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The catfish family Heptapteridae (order Siluriformes) is endemic to the Neotropics and is one of the most common of the fish families in small bodies of water. Although over 200 species have been identified in this family, very few have been characterized cytogenetically. Here, we analyze the chromosome genomes of four species of Heptapteridae: Cetopsorhamdia iheringi (Schubart & Gomes, 1959), 2n = 58, comprising 28 metacentric (m) + 26 submetacentric (sm) + 4 subtelomeric (st) chromosomes; Pimelodella vittata (Lütken, 1874), 2n = 46, comprising 16m + 22sm + 8st; Rhamdia prope quelen (Quoy & Gaimard, 1824), 2n = 58 comprising 26m + 16sm + 14st + 2 acrocentric; and Rhamdiopsis prope microcephala (Lütken, 1874), 2n = 56, comprising 12m + 30sm + 14st. The nucleolus organizer regions (NORs) were located in a single chromosome pair in all species. The two species that belonged to the subclade Nemuroglanis, Cetopsorhamdia iheringi and Rhamdia prope quelen, had a diploid chromosome number of 58 and an interstitial NOR adjacent to a C+ block located on one of the larger chromosome pairs in the complement. Our results from conventional cytogenetic techniques in combination with FISH using 18S and 5S rDNA probes corroborated the taxonomical hypothesis for the formation of the Nemuroglanis subclade.

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First cytogenetic studies of the genus Heptapterus (Actinopterygii, Siluriformes): karyotype differentiation and review of cytogenetic data on the Heptapteridae family

Cited by 5 publications

References 48 publications

Karyotype diversity and evolutionary trends in the Asian swamp eel Monopterus albus (Synbranchiformes, Synbranchidae): a case of chromosomal speciation?

Karyotype diversity and evolutionary trends in the Asian swamp eel Monopterus albus (Synbranchiformes, Synbranchidae): a case of chromosomal speciation?

Cytogenetic Trends in Two Families of the Neotropical Catfishes: Heptapteridae and Pseudopimelodidae (Siluriformes)

Cytogenetic study of heptapterids (Teleostei, Siluriformes) with particular respect to the Nemuroglanis subclade

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