Fire ant venom allergy

Dr, Hoffman

doi:10.1111/j.1398-9995.1995.tb01196.x

Cited by 76 publications

(73 citation statements)

References 65 publications

(36 reference statements)

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“…Anaphylaxis due to S. invicta venom has been reported in the USA and Spain, some cases of which have been fatal (26,27). In vitro studies demonstrated that the patient was sensitized to the Sol i 2 allergen (27).…”

Section: Introductionmentioning

confidence: 99%

“…Allergic symptoms appear in the form of sterile pustules, urticaria, edema and dermal necrosis in the absence of IgE responses; anaphylactic shock and death may occur in rare cases (20,21). The protein components in S. invicta venom contain four major allergic proteins, Sol i 1 to 4, with molecular weights of approximately 37,28,26, and 20 kDa, respectively (3). These proteins induce specific IgE responses in allergic victims.…”

Section: Introductionmentioning

confidence: 99%

“…Sol i 4 comprises 8% to 10% of the most basic protein components (isoelectric point, 10.08). It has 35% identity to Sol i 2 but does not cross-react immunologically (25).Anaphylaxis due to S. invicta venom has been reported in the USA and Spain, some cases of which have been fatal (26,27). In vitro studies demonstrated that the patient was sensitized to the Sol i 2 allergen (27).…”

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confidence: 99%

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Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata

Sukprasert¹,

Uawonggul²,

Jamjanya³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Sol i 2 is a potent allergen in Solenopsis invicta venom, and most humans exhibit reactivity to it. The Sol gem 2 allergen found in the venom of the Thai tropical fire ant Solenopsis geminata was analysed in the present study. The protein was present in higher amounts than other proteins, as determined by SDS-PAGE, and presumably has allergenic properties similar to those of Sol i 2. Sol gem 2 molecular weight is 28 and 15 kDa, respectively, under non-reducing and reducing conditions, indicating that its native form is a dimer. LC-MS/MS analysis confirmed its similarity to Sol i 2. The mono/dimeric form of Sol gem 2 was determined to be relevant by proteomic approach and immunoblotting. An anti-Sol gem 2 antibody was produced in mice, with a titer greater than 1:800 according to the Western blotting analysis. The Sol gem 2-neutralising activity of this antibody was determined in crickets. The paralytic dose 50 (PD 50 ) of crude S. geminata venom was elevated from 0.18 mg/g of body weight to more than 0.90 mg/g of body weight after preincubation with antibody at a ratio of 1:1. These results suggest that Sol gem 2 plays an important role in mediating the effects of the piperidine derivatives in the venom.Key words: allergy, ant venom, hymenoptera, immunoreactivity. Original PaPer The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2012 | volume 18 | issue 3 | pages 325-334 INTRODUCTIONVenomous insects such as bees, wasps, hornets, yellow jackets and fire ants belong to the order Hymenoptera. These insects have a stinging apparatus at the tail end of their abdominal segment and still comprise a serious problem in tropical climates. Every year, a large number of people are stung by these insects (1). The stings are capable of delivering between 100 ng (fire ants) and 50 μg (bees) of venom (2, 3). The venom is injected from a gland through the stinger and is used to catch prey and to defend against predators. The venom of these insects is composed of various biologically active peptide and protein components, some of which can cause toxicity or anaphylaxis in humans (4).Fire ants (Solenopsis sp.) are members of the Formicidae family. The medically important aggressive species are S. invicta, S. richteri, S. geminata and S. saevissima (5, 6). The venom components of these ants and their immunoreactivity have been extensively studied. Approximately 95% of fire ant venom consists of water-insoluble piperidine alkaloids, and approximately 0.1% of its weight (10 to 100 ng of protein in 0.04 to 0.11 μL per sting) is protein (7). The toxicity of the venom is thought to be due to solenopsins and methyl-, alkylor alkenyl-substituted piperidines (7-9). The venom has cytotoxic, insecticidal, antibiotic and antimicrobial properties as well (7,(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). The protein components of venom, which are wellknown allergens, are believed to play important roles in accelerating the spread of solenopsins to nearby cells and tissues. Solenopsins are believed t...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata

Sukprasert¹,

Uawonggul²,

Jamjanya³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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“…5 In those with anaphylactic reactions to red and black fire ant stings, it may produce generalized urticaria, angioedema, wheezing and hypotension. 4,6 No scar or residue is seen. In fact, only two, minute, hemorrhagic puncta may be seen, indicating the site of attachment of the mandibles.…”

Section: Discussionmentioning

confidence: 99%

“…It may pivot and sting many times in a circular pattern. 4 The usual reaction to a sting in nonsensitive individuals is a fiery, sharp pain followed by a wheal and flare response. Within hours, a clear vesicle appears that becomes pustular after about 24 hours.…”

Section: Discussionmentioning

confidence: 99%