Fingerstick Helicobacter pylori antibody test: better than laboratory serological testing?

Laine, Loren; Knigge, Kandice L.; Faigel, Douglas O.; Margaret, Nathan; Marquis, Scott; Vartan, Georgette; Fennerty, M. Brian

doi:10.1016/s0002-9270(99)00569-9

Cited by 10 publications

(4 citation statements)

References 10 publications

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“…Several previous studies have evaluated other rapid whole blood test kits for H. pylori antibody detection, reporting sensitivities and specificities of 80.3–89.5 % and 78.0–93.5 %, and PPVs and NPVs of 83–92.9 % and 57.4–93.5 %, respectively [ 13 , 17 – 19 ].…”

Section: Discussionmentioning

confidence: 99%

Performance evaluation of a rapid whole-blood immunoassay for the detection of IgG antibodies against Helicobacter pylori in daily clinical practice

Enko

Halwachs‐Baumann

Stolba

et al. 2016

Ann Clin Microbiol Antimicrob

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BackgroundA growing number of rapid Helicobacter pylori antibody tests are commercially available now, however, some of these tests are often used without sufficient evaluation. The aim of this study was to evaluate the performance of a commercially available rapid whole-blood immunoassay (gabControl®H. pylori; gabmed GmbH, Köln, Germany), for the qualitative detection of IgG antibodies against H. pylori with the 13C-urea breath test (13C-UBT) serving as a reference method.MethodsA total of 108 consecutive outpatients, who were referred for 13C-UBT by general practitioners and specialists, were also tested for H. pylori infection by the gabControl®H. pylori immunoassay. The clinical performance of this rapid whole-blood test was evaluated by determining the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) compared to the 13C-UBT. The agreement between the two tests was calculated using Cohen’s Kappa (κ) with 95 % confidence intervals (CI).ResultsThe agreement between the gabControl®H. pylori assay and the 13C-UBT was 0.62 [95 % confidence intervals (CIs) 0.47–0.76; P < 0.001]. With the 13C-UBT serving as the non-invasive gold standard method of H. pylori diagnosis, the gabControl®H. pylori assay demonstrated a sensitivity and specificity of 91.4 and 76.7 %, respectively, with a PPV of 65.3 % and a NPV of 94.9 %. Seventeen (15.7 %) individuals with a positive H. pylori anamnesis showed a negative 13C-UBT and were typed positive by the gabControl®H. pylori assay. Of these, 13 (76.5 %) and 3 individuals (17.6 %) had completed one and two eradication therapies, respectively.ConclusionsThe gabControl®H. pylori immunoassay is a rapid and easy to use first line screening tool for H. pylori IgG antibody detection in daily clinical practice. However, this assay should not be used for confirmation of the successful H. pylori eradication after antibiotic treatment.

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Section: Discussionmentioning

confidence: 99%

Performance evaluation of a rapid whole-blood immunoassay for the detection of IgG antibodies against Helicobacter pylori in daily clinical practice

Enko

Halwachs‐Baumann

Stolba

et al. 2016

Ann Clin Microbiol Antimicrob

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“…While the accuracy of first-generation tests was lower than other diagnostic tests [12] some of the recent studies of whole blood near patient tests show encouraging resultsm [16 -23] with sensitivity from 58 % to 95 % and specificity from 85 % to 98 % depending from the used test.…”

Section: Discussionmentioning

confidence: 99%

“…Whole blood immunoassays for the detection of H pylori are presently being promoted for use in primary care. Whole blood fingerstick antibody tests are simple, in-office tests providing rapid results but the accuracy of first-generation tests was lower than other diagnostic tests [12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a New Immunochromatographic Based Whole Blood Near Patient Test for the Diagnosis of Helicobacter pylori Infection

Langhorst

Heuer²,

Drouven³

et al. 2002

Z Gastroenterol

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“…It is a next-generation in-office, whole-blood antibody test that can achieve a sensitivity and specificity similar to or better than those of widely used quantitative laboratory serological tests and may be used as the initial screening tests of choice for H. pylori (Laine, et al 1999). However, its results are very poor and cannot be equated with ELISA serology (Hunt, et al 2010).…”

Section: Finger-stick Serology Testmentioning

confidence: 99%

Helicobacter pylori infection in Egypt: A review on the epidemiology, mode of transmission, diagnosis, and management of the infection.

Abdelkader

Hanora

Enany³

et al. 2021

Records of Pharmaceutical and Biomedical Sciences

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Helicobacter pylori, previously known as Campylobacter pylori, is the most famous causative agent of stomach ulcer, duodenal ulcer, gastritis, and gastric cancer and it has a direct relation with other gastric malignancies. It is a ubiquitous organism that is present in a percent of 30-100% of the global population. This percentage varies according to different geographical locations. Clinical manifestation, transmission, method of diagnosis, management of the infection, and even the treatment differs from region to region. In this review, we are concentrating on the above-mentioned factors that affect H. pylori infection epidemiology and we are directing the spotlight towards the epidemiology of this bacterium in Egypt. We have summarized different tools of diagnosis and the most famous modes of transmission of this infection in Egypt and mentioned our recommendations for future research investigation in these aspects. For example, using pulsedfield gel electrophoresis and next-generation sequencing for the full genomic characterization of H. pylori samples from this geographical area.

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Fingerstick Helicobacter pylori antibody test: better than laboratory serological testing?

Abstract: New generation in-office, whole-blood antibody tests that can achieve a sensitivity and specificity similar to or better than those of widely used quantitative laboratory serological tests may be used as the initial screening tests of choice for H. pylori.

Cited by 10 publications

References 10 publications

Performance evaluation of a rapid whole-blood immunoassay for the detection of IgG antibodies against Helicobacter pylori in daily clinical practice

Performance evaluation of a rapid whole-blood immunoassay for the detection of IgG antibodies against Helicobacter pylori in daily clinical practice

Evaluation of a New Immunochromatographic Based Whole Blood Near Patient Test for the Diagnosis of Helicobacter pylori Infection

Helicobacter pylori infection in Egypt: A review on the epidemiology, mode of transmission, diagnosis, and management of the infection.

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