Fimbrial Genes in Pseudomonas aeruginosa and Pseudomonas putida

Filloux, Alain; Bentzmann, Sophie de; Aurouze, Marianne; Lazdunski, Andrée; Vallet-Gély, Isabelle

doi:10.1007/978-1-4419-9086-0_24

Cited by 13 publications

(18 citation statements)

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“…These genes encode two specialized periplasmic chaperones (CupA2 and CupA5), the OM assembly platform, referred to as the usher (CupA3), and assembled fimbrial subunits: the CupA1 subunit, which probably forms the pilus rod and is produced in large amounts, and the CupA4 subunit, which acts as the adhesin, with one molecule of this subunit probably assembled at the tip of the fimbriae. In addition to demonstrating the existence of this CU pathway through phenotypic screening, P. aeruginosa genome mining also identified several other CU clusters (Giraud et al ., 2010) – the cupB and cupC (Vallet et al ., 2001; Ruer et al ., 2007; 2008), cupF and cupE (Filloux et al ., 2004; Giraud et al ., 2011) clusters in the PAO1 genome sequence – and the cupD cluster in the PAPI‐I pathogenicity island of the PA14 genome sequence (He et al .,2004; Mikkelsen et al ., 2009; Nicastro et al ., 2009) (Fig. 1).…”

Section: Cu Pathway In Pseudomonas Aeruginosamentioning

confidence: 99%

“…The CupA, CupB, CupC and CupD systems are found in the γ4‐fimbrial clade of the FUP (fimbrial usher protein) family (Nuccio and Bäumler, 2007). The cupA, cupC and cupD gene clusters encode an usher (CupA3, CupC3, CupD3), one or two chaperones (CupA2 and CupA5, CupC2, CupD2 and CupD5) and at least one classical fimbrial subunit (CupA1, CupC1, CupD1) (Filloux et al ., 2004; Giraud et al ., 2010). The cupB gene cluster encodes an OM mosaic protein involved in both fimbrial subunit assembly and type Vb substrate secretion from the two‐partner secretion (TPS) family renamed P‐Usher (CupB3) (Ruer et al ., 2008), two chaperones (CupB2 and CupB4), one classical fimbrial subunit (CupB1), one adhesin (CupB6) and one TPS substrate (CupB5).…”

Section: Cu Pathway In Pseudomonas Aeruginosamentioning

confidence: 99%

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Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems

Giraud

Bentzmann

2011

Environmental Microbiology

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SummaryPseudomonas aeruginosa assembles several cell surface-associated organelles, including those of the chaperone usher (CU) pathway. Five different CU loci have been identified and characterized in various strains of P. aeruginosa. However, their potential functional redundancy, particularly in biofilm formation, is supported by the control of their expression by a complex and specific regulatory network. Here, we review recent findings relating to this network. The control exerted by this network involves transcriptional repressors and activators, a phase-variable mechanism, a second intracellular messenger (c-di-GMP) and chemosensory and twocomponent systems.

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Section: Cu Pathway In Pseudomonas Aeruginosamentioning

confidence: 99%

Section: Cu Pathway In Pseudomonas Aeruginosamentioning

confidence: 99%

Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems

Giraud

Bentzmann

2011

Environmental Microbiology

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“…The opportunistic pathogen Pseudomonas aeruginosa is equipped with at least five genes encoding TpsA‐like proteins and three loci encoding a full set of components belonging to the chaperone—usher pathway (Cup) (Vallet et al , 2001; Filloux et al , 2004a, 2004b). We described the function of two P. aeruginosa Cup systems, CupB and CupC (Ruer et al , 2007).…”

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confidence: 99%

The ‘P-usher’, a novel protein transporter involved in fimbrial assembly and TpsA secretion

Ruer

Ball

Filloux

et al. 2008

EMBO J

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We identified a new bacterial transporter, the Pseudomonas aeruginosa CupB3 protein, which is an outer membrane usher involved in pili assembly. In CupB3, the usher domain has fused during evolution with a POTRA (polypeptide-transport-associated)-like domain found in TpsB transporters of two-partner secretion systems. In TpsBs, the POTRA captures the TpsA passenger, which is then transported across the outer membrane through the TpsB beta-barrel. We named CupB3 a 'P-usher' for POTRA-like domain-containing usher. We showed that CupB3 assembles CupB1 fimbrial subunits into pili and secretes CupB5, a TpsA-like protein. The CupB3 usher domain has the function of a TpsB beta-barrel in CupB5 translocation. We revealed that the POTRA-like domain is neither essential for CupB1 fimbriae assembly nor for cell surface exposition of CupB5, but is crucial to coordinate bona fide transport of CupB1 and CupB5 through the usher domain. The P-usher defines a novel transport pathway involving a molecular machine made with old spare parts.

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“…The cupA, cupB, and cupC gene clusters encode an usher, a chaperone, and at least one fimbrial subunit (8). The major fimbrial subunits were named CupA1, CupB1, and CupC1; the chaperones were named CupA2, CupB2, and CupC2 and belonged to the FGS chaperone subfamily (30), for which the F1-G1 loop length is short in comparison with the one of the FGL subfamily (15); and the usher proteins were named CupA3, CupB3, and CupC3 ( Fig.…”

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Assembly of Fimbrial Structures inPseudomonas aeruginosa: Functionality and Specificity of Chaperone-Usher Machineries

et al. 2007

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Fimbrial or nonfimbrial adhesins assembled by the bacterial chaperone-usher pathway have been demonstrated to play a key role in pathogenesis. Such an assembly mechanism has been exemplified in uropathogenic Escherichia coli strains with the Pap and the Fim systems. In Pseudomonas aeruginosa, three gene clusters (cupA, cupB, and cupC) encoding chaperone-usher pathway components have been identified in the genome sequence of the PAO1 strain. The Cup systems differ from the Pap or Fim systems, since they obviously lack numbers of genes encoding fimbrial subunits. Nevertheless, the CupA system has been demonstrated to be involved in biofilm formation on solid surfaces, whereas the role of the CupB and CupC systems in biofilm formation could not be clearly elucidated. Moreover, these gene clusters were described as poorly expressed under standard laboratory conditions. The cupB and cupC clusters are directly under the control of a two-component regulatory system designated RocA1/S1/R. In this study, we revealed that Roc1-dependent induction of the cupB and cupC genes resulted in a high level of biofilm formation, with CupB and CupC acting with synergy in clustering bacteria for microcolony formation. Very importantly, this phenotype was associated with the assembly of cell surface fimbriae visualized by electron microscopy. Finally, we observed that the CupB and CupC systems are specialized in the assembly of their own fimbrial subunits and are not exchangeable.Formation and maturation of biofilm in Pseudomonas aeruginosa are now well documented at the molecular level and involve a large arsenal of cell surface-associated organelles, including flagella (19, 25) and type IVa (19,25) or type IVb (4) pili. More recently, putative fimbrial structures, called Cup for chaperone-usher pathway, have been identified from the P. aeruginosa genome sequence (36). The chaperone-usher pathway is a conserved process for assembling fimbriae at the surfaces of gram-negative bacteria (28,30,32,35) and involves an outer membrane protein, the usher, a periplasmic chaperone, and a fimbrial subunit (28,35). Fimbrial subunits entering the periplasm via the Sec system are bound by the chaperone and form a soluble complex. The chaperone plays a critical role in folding, stabilizing, and capping the subunit prior to polymerization into a fiber. Chaperone-fimbrial-subunit complexes are targeted to the oligomeric, pore-forming, outer membrane usher (6, 33). Assembly of fimbrial subunits into fibers is then processed by donor strand exchange (3), which leads to the growth of the fimbriae through the usher from the tip to the base (27). Finally, fimbrial structures assembled by the chaperoneusher pathway have frequently been reported as having a role in bacterial pathogenesis (14), facilitating bacterial attachment to host tissue and promoting biofilm formation (23, 30).Three gene clusters have been identified in the P. aeruginosa genome (31) and named cup (36). The cupA, cupB, and cupC gene clusters encode an usher, a chaperone, and at least on...

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Fimbrial Genes in Pseudomonas aeruginosa and Pseudomonas putida

Cited by 13 publications

References 81 publications

Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems

Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems

The ‘P-usher’, a novel protein transporter involved in fimbrial assembly and TpsA secretion

Assembly of Fimbrial Structures inPseudomonas aeruginosa: Functionality and Specificity of Chaperone-Usher Machineries

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