Filopodia in cell adhesion, 3D migration and cancer cell invasion

Jacquemet, Guillaume; Hamidi, Hellyeh; Ivaska, Johanna

doi:10.1016/j.ceb.2015.06.007

Cited by 444 publications

(408 citation statements)

References 64 publications

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“…Filopodia are thin actin-based protrusions that extend from cells involved in cell adhesion and migration (Jacquemet et al, 2015). In 1961, Wolpert and Gustafson visualized filopodia in live sea urchin embryos at the blastula stage, resulting in behavior indicating that filopodia function as sensors of patterning information (Gustafson and Wolpert, 1961).…”

Section: Cytonemes Regulate the Distribution Of Signaling Proteinsmentioning

confidence: 99%

Role of cytonemes in Wnt transport

Stanganello

Scholpp

2016

Journal of Cell Science

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Wnt signaling regulates a broad variety of processes during embryonic development and disease. A hallmark of the Wnt signaling pathway is the formation of concentration gradients by Wnt proteins across responsive tissues, which determines cell fate in invertebrates and vertebrates. To fulfill its paracrine function, trafficking of the Wnt morphogen from an origin cell to a recipient cell must be tightly regulated. A variety of models have been proposed to explain the extracellular transport of these lipid-modified signaling proteins in the aqueous extracellular space; however, there is still considerable debate with regard to which mechanisms allow the precise distribution of ligand in order to generate a morphogenetic gradient within growing tissue. Recent evidence suggests that Wnt proteins are distributed along signaling filopodia during vertebrate and invertebrate embryogenesis. Cytoneme-mediated transport has profound impact on our understanding of how Wnt signaling propagates through tissues and allows the formation of a precise ligand distribution in the recipient tissue during embryonic growth. In this Commentary, we review extracellular trafficking mechanisms for Wnt proteins and discuss the growing evidence of cytoneme-based Wnt distribution in development and stem cell biology. We will also discuss their implication for Wnt signaling in the formation of the Wnt morphogenetic gradient during tissue patterning.

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Section: Cytonemes Regulate the Distribution Of Signaling Proteinsmentioning

confidence: 99%

Role of cytonemes in Wnt transport

Stanganello

Scholpp

2016

Journal of Cell Science

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“…40 Cells migrating in the two-dimensional models often display only sparse filopodia, whereas in the threedimensional invasion model or in vivo, cells extensively use filopodia to probe the extracellular matrix and migrate. 41,42 Both cell lines exhibited mutations in PTEN, whereas U118 also revealed p53 mutation.…”

mentioning

confidence: 99%

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Wierzbicki¹,

Jaworski²,

Kutwin³

et al. 2017

IJN

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The highly invasive nature of glioblastoma is one of the most significant problems regarding the treatment of this tumor. Diamond nanoparticles (ND), graphite nanoparticles (NG), and graphene oxide nanoplatelets (nGO) have been explored for their biomedical applications, especially for drug delivery. The objective of this research was to assess changes in the adhesion, migration, and invasiveness of two glioblastoma cell lines, U87 and U118, after ND, NG, and nGO treatment. All treatments affected the cell surface structure, adhesion-dependent EGFR/AKT/mTOR, and β-catenin signaling pathways, decreasing the migration and invasiveness of both glioblastoma cell lines. The examined nanoparticles did not show strong toxicity but effectively deregulated cell migration. ND was effectively taken up by cells, whereas nGO and NG strongly interacted with the cell surface. These results indicate that nanoparticles could be used in biomedical applications as a low toxicity active compound for glioblastoma treatment.

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“…Trophozoites, like other migratory cells (Yamaguchi and Condeelis, 2007; Jacquemet et al, 2015; Fritz-Laylin et al, 2017), showed filamentous actin in their membrane protrusions when stimulated in the MPGL, generating mainly long and short filipodia. The long filipodia did not show completely structured F-actin, possibly due to the fact that it was in the process of integration to stabilize the tubular filamentous structure (Karlsson et al, 2013).…”

Section: Discussionmentioning

confidence: 94%

Influence of Micropatterned Grill Lines on Entamoeba histolytica Trophozoites Morphology and Migration

Sierra-López

Baylón-Pacheco

Espíritu-Gordillo

et al. 2018

Front. Cell. Infect. Microbiol.

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Entamoeba histolytica, the causal agent of human amoebiasis, has two morphologically different phases: a resistant cyst and a trophozoite responsible for the invasion of the host tissues such as the colonic mucosa and the intestinal epithelium. During in vitro migration, trophozoites usually produce protuberances such as pseudopods and rarely filopodia, structures that have been observed in the interaction of trophozoites with human colonic epithelial tissue. To study the different membrane projections produced by the trophozoites, including pseudopods, filopodia, uropods, blebs, and others, we designed an induction system using erythrocyte extract or fibronectin (FN) in micropatterned grill lines (each micro-line containing multiple micro-portions of FN or erythrocyte extract) on which the trophozoites were placed in culture for migration assays. Using light, confocal, and scanning electron microscopy, we established that E. histolytica trophozoites frequently produce short and long filopodia, large retractile uropods in the rear, pseudopods, blebs, and others structures, also showing continuous migration periods. The present study provides a simple migration method to induce trophozoites to generate abundant membrane protrusion structures that are rarely obtained in normal or induced cultures, such as long filopodia; this method will allow a–better understanding of the interactions of trophozoites with FN and cell debris. E. histolytica trophozoites motility plays an important role in invasive amoebiasis. It has been proposed that both physical forces and chemical signals are involved in the trophozoite motility and migration. However, the in vivo molecules that drive the chemotactic migration remain to be determined. We propose the present assay to study host molecules that guide chemotactic behavior because the method is highly reproducible, and a live image of cell movement and migration can be quantified.

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Filopodia in cell adhesion, 3D migration and cancer cell invasion

Cited by 444 publications

References 64 publications

Role of cytonemes in Wnt transport

Role of cytonemes in Wnt transport

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Influence of Micropatterned Grill Lines on Entamoeba histolytica Trophozoites Morphology and Migration

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