Filamentous Artificial Virus from a Self‐Assembled Discrete Nanoribbon

Lim, Yong Beom; Lee, Eunji; Yoon, You Rim; Lee, Myeong Sup; Lee, Myongsoo

doi:10.1002/anie.200800266

Cited by 86 publications

(56 citation statements)

References 34 publications

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“…Cationic polymers-such as linear-and branched-polyethylenimine (PEI), polyamidoamine (PAMAM) dendrimer, poly-L-lysine, cationic polyesters (Lim et al, 2001(Lim et al, , 2000, and poly-β-amino esters-have been the subject of intensive interest as nonviral genecarrier molecules. Because the polyanionic nature of nucleic acids inhibits them from crossing the same charged cytoplasmic membrane barrier, cationic polymers are used to neutralize the negative charges of nucleic acids (Lim et al, 2008).…”

Section: R E S E a R C H A R T I C L Ementioning

confidence: 99%

Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Choi

Kang

Kim

et al. 2010

Drug and Chemical Toxicology

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A safe alternative to the viral system used in gene therapy is a nonviral gene delivery system. Although polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer are among the most promising gene-carrier candidates for efficient nonviral gene delivery, safety concerns regarding their toxicity remain. The aim of this study was to scrutinize the underlying mechanism of the cytotoxicity and genotoxicity of PEI (25 kDa) and PAMAM (G4). To our knowledge, this is the first study to explore the genotoxic effect of polymeric gene carriers. To evaluate cell death by PEI and PAMAM, we performed propidium-iodide staining and lactate-dehydrogenase release assays. The genotoxicity of the polymers was measured by comet assay and cytokinesis-block micronucleus assay. PEI- and PAMAM-treated groups induced both necrotic and apoptotic cell death. In the comet assay and micronuclei formation, significant increases in DNA damage were observed in both treatments. We conclude that PEI and PAMAM dendrimer can induce not only a relatively weak apoptotic and a strong necrotic effect, but also a moderate genotoxic effect.

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Section: R E S E a R C H A R T I C L Ementioning

confidence: 99%

Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Choi

Kang

Kim

et al. 2010

Drug and Chemical Toxicology

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“…Nonetheless, Lim et al were able to synthesize a self-assembling filamentous capsid containing siRNA by using self-assembling β-sheet peptides with poly-lysine sequences for DNA binding and covalently attached glucose ligands for cell-specific receptor binding. This synthetic capsid was able to deliver siRNA and silence GFP expression in HeLa cells just as well as lipofectamine (∼70% reduction in GFP expression) [36]. Malay et al also showed that gold nanoparticles could be used to catalyze formation of capsids with cysteine rich trp RNA-binding attenuation protein (TRAP) monomers.…”

Section: Highly Evolved Hgt: Virusesmentioning

confidence: 99%

Applying horizontal gene transfer phenomena to enhance non-viral gene therapy

Elmer

Christensen

Rege

2013

Journal of Controlled Release

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Horizontal gene transfer (HGT) is widespread amongst prokaryotes, but eukaryotes tend to be far less promiscuous with their genetic information. However, several examples of HGT from pathogens into eukaryotic cells have been discovered and mimicked to improve non-viral gene delivery techniques. For example, several viral proteins and DNA sequences have been used to significantly increase cytoplasmic and nuclear gene delivery. Plant genetic engineering is routinely performed with the pathogenic bacterium Agrobacterium tumefaciens and similar pathogens (e.g. Bartonella henselae) may also be able to transform human cells. Intracellular parasites like Trypanosoma cruzi may also provide new insights into overcoming cellular barriers to gene delivery. Finally, intercellular nucleic acid transfer between host cells will also be briefly discussed. This article will review the unique characteristics of several different viruses and microbes and discuss how their traits have been successfully applied to improve non-viral gene delivery techniques. Consequently, pathogenic traits that originally caused diseases may eventually be used to treat many genetic diseases.

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“…Students were informed that the corporations were all competing for a grant that would be awarded to the most promising artificial virus. The work students completed in the course was not simply a theoretical exercise; they were investigating an issue with significant real-world implications (23).…”

Section: Project Description and Assessmentmentioning

confidence: 99%

Designing Cancer-Killing Artificial Viruses to Improve Student Understanding of Microbiology

Kuniyuki¹,

Sharp

2011

J Microbiol Biol Educ.

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Our objective was to assess the effectiveness of a "learning by designing" group project used in a lower-division Microbiology course. Students used knowledge gained from the course to design an artificial virus that would kill cancer cells. The assignment required groups to integrate the individual course topics into a unified, complex understanding of the field of microbiology. Throughout the course, students and the instructor collaborated in creating a rubric to evaluate the groups' final presentations. This paper reports the results of an assessment of the project by comparing the instructor's and the students' scores for the presentations. Students' and the instructor's scores were correlated; the Pearson coefficient of 0.52 was statistically significant. The results indicate that students gained sufficient knowledge to accurately evaluate proposed designs. Additionally, the overall course grade distribution improved compared to the semester before the project was introduced. Finally, in order to engage students in thinking about their own learning process, they completed a reflection assignment that required them to discuss the changes in their understanding of microbiology over the course of the semester. Our assessment indicates that a design project can serve as an effective and useful learning technique in undergraduate Microbiology courses, though modifications are suggested.

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Filamentous Artificial Virus from a Self‐Assembled Discrete Nanoribbon

Cited by 86 publications

References 34 publications

Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Applying horizontal gene transfer phenomena to enhance non-viral gene therapy

Designing Cancer-Killing Artificial Viruses to Improve Student Understanding of Microbiology

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